公开(公告)号：US06825325B1

公开(公告)日：2004-11-30

申请号：US09419788

申请日：1999-10-18

申请人： Rainer Fischer ,  Stefan Schillberg ,  Jörg Nähring ,  Markus Sack ,  Michael Monecke ,  Yu-Cai Liao ,  Holger Spiegel ,  Sabine Zimmerman ,  Neil Emans

发明人： Rainer Fischer ,  Stefan Schillberg ,  Jörg Nähring ,  Markus Sack ,  Michael Monecke ,  Yu-Cai Liao ,  Holger Spiegel ,  Sabine Zimmerman ,  Neil Emans

IPC分类号： C12P2108

CPC分类号： C12N15/8283 ,  C07K14/21 ,  C07K14/415 ,  C07K14/7051 ,  C07K16/10 ,  C07K2317/13 ,  C07K2317/34 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2319/00 ,  C12N15/8258

摘要： The present invention is drawn to a fusion protein containing at least one binding domain that specifically recognizes an eptitope of a plant pathogen and at least one additional domain made from a protein or peptide sequence which is toxic to the pathogen or detrimental the replication, transmission or life cycle of the pathogen. The present invention is further drawn to a pathogenocide made from the binding domain, a cellular targeting sequence and/or membrane localization sequence that leads to membrane anchoring. The present invention is further drawn to nucleotide sequences encoding fusion proteins and pathogenocides and to vectors containing the nucleotide sequences; as well as methods of making the fusion proteins and pathogencides and methods of making pathogen resistant plants, plant cells, or plant tissues with the fusion proteins and pathogenocides.

摘要翻译： 本发明涉及一种融合蛋白，其含有特异性识别植物病原体的视角的至少一个结合结构域和由对病原体有毒的蛋白质或肽序列制成的至少一个另外的结构域，或不利于复制，传播或 病原体的生命周期。 本发明进一步涉及由结合域制成的致病性杀伤剂，导致膜锚定的细胞靶向序列和/或膜定位序列。 本发明进一步涉及编码融合蛋白和病原菌的核苷酸序列和含有该核苷酸序列的载体; 以及制备融合蛋白和病原体的方法以及用融合蛋白和病原体杀伤剂制备具有抗病性的植物，植物细胞或植物组织的方法。