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    Immunoglobulin having particular framework scaffold and methods of making and using
    2.
    发明申请
    Immunoglobulin having particular framework scaffold and methods of making and using 审中-公开
    具有特定骨架支架的免疫球蛋白和制造和使用方法

    公开(公告)号：US20050037420A1

    公开(公告)日：2005-02-17

    申请号：US10489328

    申请日：2002-09-13

    申请人： Mei-Yun Zhang ,  Stefan Schillberg ,  Sabine Zimmermann ,  Stefano Fiore ,  Neil Emans ,  Rainer Fischer

    发明人： Mei-Yun Zhang ,  Stefan Schillberg ,  Sabine Zimmermann ,  Stefano Fiore ,  Neil Emans ,  Rainer Fischer

    IPC分类号： C07K16/00 ,  C07K16/08 ,  G01N33/53 ,  C07K16/44

    CPC分类号： C07K16/081 ,  A01K2217/05 ,  C07K16/00 ,  C07K2317/13 ,  C07K2317/23 ,  C07K2317/24 ,  C07K2317/567 ,  C07K2317/622

    摘要： This invention relates to immunoglobulin molecules comprising light chain (VL) chimeric variable domains, heavy chain (VH) chimeric variable domains, e.g., scFv antibodies that are expressed at high levels within a host cell, preferably within particular cellular compartments such as, e.g., cytosol or apoplast. The VL, VH and scFv antibody molecules comprise framework scaffolds of particularly preferred framework regions. This invention also relates to nucleic acid molecules encoding the immunoglobulin molecules of this invention, vectors expressing the immunoglobulin molecules, hosts transformed with the nucleic acid molecules and vectors, and methods of using the immunoglobulin molecules. Also described are immunoglobulin libraries as well as host cells, including transgenic plants, expressing the VL, VH or scFv antibody molecules of this invention.

    摘要翻译： 本发明涉及包含轻链（VL）嵌合可变域，重链（VH）嵌合可变结构域的免疫球蛋白分子，例如在宿主细胞内，优选在特定细胞区室内以高水平表达的scFv抗体，例如， 胞质或质外体。 VL，VH和scFv抗体分子包括特别优选的框架区的框架支架。 本发明还涉及编码本发明的免疫球蛋白分子的核酸分子，表达免疫球蛋白分子的载体，用核酸分子和载体转化的宿主，以及使用免疫球蛋白分子的方法。 还描述了免疫球蛋白文库以及表达本发明的VL，VH或scFv抗体分子的宿主细胞，包括转基因植物。

    METHOD FOR THE GENERATION OF A MONOCLONAL PLANT CELL LINE
    3.
    发明申请
    METHOD FOR THE GENERATION OF A MONOCLONAL PLANT CELL LINE 审中-公开
    单体植物细胞系生成方法

    公开(公告)号：US20140053300A1

    公开(公告)日：2014-02-20

    申请号：US14115629

    申请日：2011-06-07

    申请人： Janina Kirchhoff ,  Stefan Schillberg ,  Andreas Schiermeyer ,  Helga Schinkel ,  Rainer Fischer

    发明人： Janina Kirchhoff ,  Stefan Schillberg ,  Andreas Schiermeyer ,  Helga Schinkel ,  Rainer Fischer

    IPC分类号： C12N15/82

    摘要： The invention provides a method for the generation of a monoclonal plant cell line from a heterologous population of plant cells, comprising the following steps: (a) provision of a heterologous population of plant cells; (b) preparation of protoplasts from said heterologous population of plant cells; (c) separation of single protoplasts by subjecting the preparation of protoplasts to flow cytometric sorting; (d) regeneration of a separated single transformed protoplast until the formation of a microcolony by co-cultivation in the presence of feeder cell material; (e) removal of the microcolony from the feeder cell material and cultivation of the microcolony until the formation of a monoclonal plant cell line.

    摘要翻译： 本发明提供了从异源植物细胞群体产生单克隆植物细胞系的方法，包括以下步骤：（a）提供异源植物细胞群; （b）从所述异源植物细胞群体制备原生质体; （c）通过使原生质体的制备物流式细胞分选来分离单个原生质体; （d）分离的单一转化的原生质体的再生直到在饲养细胞材料存在下共培养形成微菌落; （e）从饲养细胞材料中除去微菌落并培养微菌落直到形成单克隆植物细胞系。