公开(公告)号：US20100247570A1

公开(公告)日：2010-09-30

申请号：US12514312

申请日：2007-11-13

申请人： Yvonne Rosenberg ,  Markus Sack ,  Rainer Fischer

发明人： Yvonne Rosenberg ,  Markus Sack ,  Rainer Fischer

IPC分类号： A61K39/21 ,  A61P31/12 ,  A61P31/18 ,  C12N15/09

CPC分类号： A61K39/21 ,  A61K39/12 ,  A61K2039/517 ,  C12N2740/16134

摘要： Glycoproteins containing high mannose oligosaccharides are obtained and used to generate antibody to the mannose portion of the molecule. Such antibodies can be used in a diagnostic assay. The glycoproteins can be used to generate neutralizing antibody.

摘要翻译： 获得含有高甘露糖寡糖的糖蛋白，并用于产生对分子的甘露糖部分的抗体。 这样的抗体可用于诊断测定。 糖蛋白可用于产生中和抗体。

公开(公告)号：US06825325B1

公开(公告)日：2004-11-30

申请号：US09419788

申请日：1999-10-18

申请人： Rainer Fischer ,  Stefan Schillberg ,  Jörg Nähring ,  Markus Sack ,  Michael Monecke ,  Yu-Cai Liao ,  Holger Spiegel ,  Sabine Zimmerman ,  Neil Emans

发明人： Rainer Fischer ,  Stefan Schillberg ,  Jörg Nähring ,  Markus Sack ,  Michael Monecke ,  Yu-Cai Liao ,  Holger Spiegel ,  Sabine Zimmerman ,  Neil Emans

IPC分类号： C12P2108

CPC分类号： C12N15/8283 ,  C07K14/21 ,  C07K14/415 ,  C07K14/7051 ,  C07K16/10 ,  C07K2317/13 ,  C07K2317/34 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2319/00 ,  C12N15/8258

摘要： The present invention is drawn to a fusion protein containing at least one binding domain that specifically recognizes an eptitope of a plant pathogen and at least one additional domain made from a protein or peptide sequence which is toxic to the pathogen or detrimental the replication, transmission or life cycle of the pathogen. The present invention is further drawn to a pathogenocide made from the binding domain, a cellular targeting sequence and/or membrane localization sequence that leads to membrane anchoring. The present invention is further drawn to nucleotide sequences encoding fusion proteins and pathogenocides and to vectors containing the nucleotide sequences; as well as methods of making the fusion proteins and pathogencides and methods of making pathogen resistant plants, plant cells, or plant tissues with the fusion proteins and pathogenocides.

摘要翻译： 本发明涉及一种融合蛋白，其含有特异性识别植物病原体的视角的至少一个结合结构域和由对病原体有毒的蛋白质或肽序列制成的至少一个另外的结构域，或不利于复制，传播或 病原体的生命周期。 本发明进一步涉及由结合域制成的致病性杀伤剂，导致膜锚定的细胞靶向序列和/或膜定位序列。 本发明进一步涉及编码融合蛋白和病原菌的核苷酸序列和含有该核苷酸序列的载体; 以及制备融合蛋白和病原体的方法以及用融合蛋白和病原体杀伤剂制备具有抗病性的植物，植物细胞或植物组织的方法。

公开(公告)号：US20050059053A1

公开(公告)日：2005-03-17

申请号：US10895399

申请日：2004-07-21

申请人： Rainer Fischer ,  Markus Sack ,  Carmen Vaquero-Martin

发明人： Rainer Fischer ,  Markus Sack ,  Carmen Vaquero-Martin

IPC分类号： C07H21/04 ,  C07K14/705 ,  C07K16/30 ,  C12N15/62 ,  C12N15/82 ,  C12P21/02 ,  C12Q1/68 ,  C12P21/04 ,  G01N33/53

CPC分类号： C12N15/62 ,  C07H21/04 ,  C07K14/70503 ,  C07K16/3007 ,  C07K2317/13 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2319/033 ,  C07K2319/21 ,  C07K2319/40 ,  C12N15/8257 ,  C12N15/8258 ,  C12P21/02

摘要： A method is described for altering the properties such as the accumulation, the stability and/or integrity, the subcellular localisation, the post-translational modifications, the ability to get purified, and the phase partitioning behaviour of natural or recombinant target proteins expressed in a host organism. The method involves the co-expression of natural or recombinant proteins along with a specific binding partner that sequesters the target recombinant protein into a complex. The binding partner is supplied as a separate protein allowing formation of intermolecular complexes or is fused to the protein of interest, allowing the formation of intramolecular complexes. The binding partner can also be used to alter the subcellular localisation without modifying the sequence or structure of the target protein itself. This can be achieved by either incorporating appropriate targeting signals into the binding ligand, which are then linked to the target protein through complex formation, or complex formation itself may alter the subcellular localisation. The same strategy can be used to provide an affinity tag to facilitate protein purification. The principle of the invention is demonstrated by the coexpression of an unstable antibody and its cognate antigen.

摘要翻译： 描述了一种用于改变诸如累积，稳定性和/或完整性，亚细胞定位，翻译后修饰，获得纯化的能力以及表达于天然或重组靶蛋白的相分离行为的性质的方法 宿主生物。 该方法包括将天然或重组蛋白与特异性结合配偶体共表达，所述特异性结合配偶体将靶重组蛋白质螯合到复合物中。 结合配偶体作为单独的蛋白质提供，允许形成分子间复合物或者与感兴趣的蛋白质融合，从而形成分子内复合物。 结合配偶体也可用于改变亚细胞定位，而不改变靶蛋白本身的序列或结构。 这可以通过将适当的靶向信号并入结合配体中来实现，然后通过复合物形成与靶蛋白连接，或者复合物形成本身可能改变亚细胞定位。 可以使用相同的策略来提供亲和标签以促进蛋白质纯化。 本发明的原理通过不稳定抗体及其同源抗原的共表达来证明。