公开(公告)号：US6030791A

公开(公告)日：2000-02-29

申请号：US153304

申请日：1998-09-15

申请人： Scott M. Braxton ,  Dinh Diep ,  Angelo M. Delegeane

发明人： Scott M. Braxton ,  Dinh Diep ,  Angelo M. Delegeane

IPC分类号： A61K38/00 ,  C12N9/64 ,  G01N33/53 ,  A61K39/395 ,  C07K14/52

CPC分类号： C12N9/6424 ,  A61K38/00

摘要： The present invention provides nucleotide and amino acid sequences that identify and encode a human homolog of rat elastase IV (HEIV) expressed in pancreas tissue. The present invention also provides for antisense molecules to the nucleotide sequences which encode HEIV, hybridization probes or oligonucleotides for the detection of HEIV-encoding nucleotide sequences, and a diagnostic test based on HEIV-encoding nucleic acid molecules. The present invention further provides for genetically engineered host cells for the expression of HEIV, biologically active HEIV, antibodies capable for binding specifically to HEIV, and treatment methods comprising administration of compounds, such antibodies or inhibitors, capable of binding HEIV to alter its activity.

摘要翻译： 本发明提供了鉴定和编码在胰腺组织中表达的大鼠弹性蛋白酶IV（HEIV）的人同源物的核苷酸和氨基酸序列。 本发明还提供编码用于检测编码HEIV的核苷酸序列的HEIV，杂交探针或寡核苷酸的核苷酸序列的反义分子，以及基于编码HEIV的核酸分子的诊断测试。 本发明还提供用于表达HEIV，生物活性HEIV，能够特异性结合HEIV的抗体的基因工程宿主细胞，以及包含能够结合HEIV以改变其活性的化合物，例如抗体或抑制剂的治疗方法。

公开(公告)号：US5856109A

公开(公告)日：1999-01-05

申请号：US966319

申请日：1997-11-07

申请人： Scott M. Braxton ,  Dinh Diep ,  Angelo M. Delegeane

发明人： Scott M. Braxton ,  Dinh Diep ,  Angelo M. Delegeane

IPC分类号： A61K38/00 ,  C12N9/64 ,  G01N33/53 ,  C07K1/00

CPC分类号： C12N9/6424 ,  A61K38/00

摘要： The present invention provides nucleotide and amino acid sequences that identify and encode a human homolog of rat elastase IV (HEIV) expressed in pancreas tissue. The present invention also provides for antisense molecules to the nucleotide sequences which encode HEIV, hybridization probes or oligonucleotides for the detection of HEIV-encoding nucleotide sequences, and a diagnostic test based on HEIV-encoding nucleic acid molecules. The present invention further provides for genetically engineered host cells for the expression of HEIV, biologically active HEIV, antibodies capable for binding specifically to HEIV, and treatment methods comprising administration of compounds, such antibodies or inhibitors, capable of binding HEIV to alter its activity.

摘要翻译： 本发明提供了鉴定和编码在胰腺组织中表达的大鼠弹性蛋白酶IV（HEIV）的人同源物的核苷酸和氨基酸序列。 本发明还提供编码用于检测编码HEIV的核苷酸序列的HEIV，杂交探针或寡核苷酸的核苷酸序列的反义分子，以及基于编码HEIV的核酸分子的诊断测试。 本发明还提供用于表达HEIV，生物活性HEIV，能够特异性结合HEIV的抗体的基因工程宿主细胞，以及包含能够结合HEIV以改变其活性的化合物，例如抗体或抑制剂的治疗方法。

公开(公告)号：US5637462A

公开(公告)日：1997-06-10

申请号：US426428

申请日：1995-04-19

申请人： Roger Coleman ,  Scott M. Braxton ,  Jeffrey J. Seilhamer

发明人： Roger Coleman ,  Scott M. Braxton ,  Jeffrey J. Seilhamer

IPC分类号： G01N33/50 ,  C07K14/60 ,  C12N9/48 ,  C12N15/09 ,  C12N15/57 ,  C12Q1/34 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/566 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12N9/48

摘要： The present invention provides nucleotide and amino acid sequences that identify and encode a new cathepsin C homolog (RCP) expressed in THP-1 cells. The present invention also provides for antisense molecules to the nucleotide sequences which encode RCP, expression vectors for the production of purified RCP, antibodies capable of binding specifically to RCP, hybridization probes or oligonucleotides for the detection of RCP-encoding nucleotide sequences, genetically engineered host cells for the expression of RCP, diagnostic tests for activation of monocyte/macrophages based on RCP-encoding nucleic acid molecules, and use of the protein to produce antibodies capable of binding specifically to the protein and use of the protein to screen for inhibitors.

摘要翻译： 本发明提供鉴定和编码在THP-1细胞中表达的新的组织蛋白酶C同系物（RCP）的核苷酸和氨基酸序列。 本发明还提供编码RCP的核苷酸序列的反义分子，用于产生纯化的RCP的表达载体，能够特异性结合RCP的抗体，用于检测编码RCP的核苷酸序列的杂交探针或寡核苷酸的遗传工程宿主 用于表达RCP的细胞，用于基于编码RCP的核酸分子激活单核细胞/巨噬细胞的诊断测试，以及使用该蛋白质产生能够特异性结合蛋白质的抗体和使用蛋白质筛选抑制剂。

公开(公告)号：US5766897A

公开(公告)日：1998-06-16

申请号：US427100

申请日：1995-04-21

申请人： Scott M. Braxton

发明人： Scott M. Braxton

IPC分类号： A61K38/00 ,  C07K1/107 ,  C07K14/81 ,  C12N15/00 ,  C12N9/48 ,  C12N9/50 ,  C12N9/96

CPC分类号： C07K1/1077 ,  C07K14/8121 ,  A61K38/00

摘要： Methods and compositions are provided for the production of PEGylated proteins having polyethylene glycol covalently bound to a cysteine residue present in either the naturally-occurring protein or introduced by site-specific mutation. Where the cysteine residue is introduced by mutation, the site for mutation is selected on the basis of the presence of an N-linked glycosylation site or the position of the residue which is normally solvent-accessible in the naturally-occurring protein. The modified proteins produced by the method of the invention are referred to as cysteine-PEGylated proteins. Proteins PEGylated according to the invention have increased half-lives following administration to a subject and decreased immunogenicity and antigenicity, while retaining substantially the same level of biological activity as that of the naturally-occurring, unmodified protein. Modification of proteins according to methods of the invention thus provide improved pharmaceutical compositions.

摘要翻译： 提供了用于生产聚乙二醇化蛋白质的聚乙二醇化蛋白质，所述聚乙二醇化蛋白质具有与存在于天然蛋白质中的半胱氨酸残基共价结合或通过位点特异性突变引入的聚乙二醇。 当通过突变引入半胱氨酸残基时，基于N-连接的糖基化位点的存在或通常在天然存在的蛋白质中可溶解的残基的位置来选择突变位点。 通过本发明的方法产生的经修饰的蛋白质被称为半胱氨酸 - 聚乙二醇化蛋白质。 根据本发明聚乙二醇化的蛋白质在施用于受试者后具有增加的半衰期并降低免疫原性和抗原性，同时保持与天然存在的未修饰蛋白基本相同的生物活性水平。 因此，根据本发明的方法，蛋白质的修饰因此提供了改进的药物组合物。