公开(公告)号：US20100292471A1

公开(公告)日：2010-11-18

申请号：US12299665

申请日：2007-12-17

申请人： Hee Kyung Park ,  Jong Chan Lim ,  Sung Jin Ha ,  Serka Kim ,  Jae Jin Choi ,  Jung Hyun Min ,  Jin Won Yun

发明人： Hee Kyung Park ,  Jong Chan Lim ,  Sung Jin Ha ,  Serka Kim ,  Jae Jin Choi ,  Jung Hyun Min ,  Jin Won Yun

IPC分类号： C07D473/18

CPC分类号： C07H21/00 ,  C07K14/003

摘要： Disclosed is a PNA oligomer with increased solubility in water and specificity upon hybridization with nucleic acid, which comprises at least one universal base, capable of forming a base pair with natural DNA or RNA bases, incorporated in its base sequence, the base sequence having at least 60% purine bases or at least four contiguous purine bases.

摘要翻译： 公开了一种具有增加的在水中的溶解度和与核酸杂交的特异性的PNA低聚物，该核酸包含至少一个通用碱基，其能够与天然的DNA或RNA碱基形成碱基对，并且其碱基序列具有 至少60％的嘌呤碱基或至少四个相邻的嘌呤碱基。

公开(公告)号：US20080248461A1

公开(公告)日：2008-10-09

申请号：US11851581

申请日：2007-09-07

申请人： Hee Kyung Park ,  Hyunil Lee ,  Jae Jin Choi ,  Serka Kim

发明人： Hee Kyung Park ,  Hyunil Lee ,  Jae Jin Choi ,  Serka Kim

IPC分类号： C12Q1/70 ,  C07H21/00

CPC分类号： C12Q1/708 ,  C12Q2525/107

摘要： Disclosed are PNA probes capable of genotype specifically binding with Human Paillomavirus (HPV) DNA, kits for detecting HPV genotypes comprising the probes, and methods for detecting HPV genotypes by using the kits, which enables the accurate detection of all 24 genotypes of HPV found in cervix, diagnosis of combined infection with more than one HPV genotype, and detection of HPV genotypes with high specificity and sensitivity.

摘要翻译： 公开了能够基因型与人类苍蝇病毒（HPV）DNA特异性结合的PNA探针，用于检测包含探针的HPV基因型的试剂盒，以及通过使用试剂盒检测HPV基因型的方法，其能够精确检测所有24种HPV基因型 子宫颈，联合感染多种HPV基因型的诊断，以及具有高特异性和敏感性的HPV基因型的检测。

公开(公告)号：US08133985B2

公开(公告)日：2012-03-13

申请号：US12300235

申请日：2007-12-14

申请人： Hyunil Lee ,  Hee Kyung Park ,  Bong Ho Um ,  Serka Kim ,  Jae Jin Choi ,  Jung Hyun Min ,  Hye Yeon Kim

发明人： Hyunil Lee ,  Hee Kyung Park ,  Bong Ho Um ,  Serka Kim ,  Jae Jin Choi ,  Jung Hyun Min ,  Hye Yeon Kim

IPC分类号： C07K2/00 ,  C07K1/00 ,  C40B80/00 ,  C40B40/06

CPC分类号： C07K14/003 ,  A61K47/641 ,  Y10S977/754

摘要： The present invention relates to a peptide nucleic acid (PNA) conjugated with multi-amine linkers, and a method to prepare the same and utilization thereof. More specifically, the method is characterized by conjugating monomers having multi-amine functionality sequentially at a PNA terminal, and effectively immobilizing the PNA conjugated with multi-amine linkers on a solid surface. A PNA array prepared using the PNA conjugated with multi-amine linkers exhibits improved sensitivity and specificity of signals for detecting target nucleic acids as compared to a PNA array using PNA probes having only one amine group. The PNA conjugated with multi-amine linkers can be utilized in nucleic acid detecting devices or kits for gene diagnosis such as PNA microarrays, PNA chips, PNA field-effect transistors and impedance detectors.

摘要翻译： 本发明涉及与多胺接头共轭的肽核酸（PNA）及其制备方法及应用。 更具体地说，该方法的特征在于在PNA末端连续连接具有多胺官能团的单体，并有效地将与多胺接头共轭的PNA固定在固体表面上。 使用与多胺接头共轭的PNA制备的PNA阵列与使用仅具有一个胺基团的PNA探针的PNA阵列相比，显示出用于检测靶核酸的信号的改善的灵敏度和特异性。 与多胺接头结合的PNA可用于核酸检测装置或用于基因诊断的试剂盒，例如PNA微阵列，PNA芯片，PNA场效应晶体管和阻抗检测器。

公开(公告)号：US20090312197A1

公开(公告)日：2009-12-17

申请号：US12300235

申请日：2007-12-14

申请人： Hyunil Lee ,  Hee Kyung Park ,  Bong Ho Um ,  Serka Kim ,  Jae Jin Choi ,  Jung Hyun Min ,  Hye Yeon Kim

发明人： Hyunil Lee ,  Hee Kyung Park ,  Bong Ho Um ,  Serka Kim ,  Jae Jin Choi ,  Jung Hyun Min ,  Hye Yeon Kim

IPC分类号： C40B40/08 ,  C07K9/00 ,  C07K1/00 ,  C40B50/14

CPC分类号： C07K14/003 ,  A61K47/641 ,  Y10S977/754

摘要： The present invention relates to a peptide nucleic acid (PNA) conjugated with multi-amine linkers, and a method to prepare the same and utilization thereof. More specifically, the method is characterized by conjugating monomers having multi-amine functionality sequentially at a PNA terminal, and effectively immobilizing the PNA conjugated with multi-amine linkers on a solid surface. A PNA array prepared using the PNA conjugated with multi-amine linkers exhibits improved sensitivity and specificity of signals for detecting target nucleic acids as compared to a PNA array using PNA probes having only one amine group. The PNA conjugated with multi-amine linkers can be utilized in nucleic acid detecting devices or kits for gene diagnosis such as PNA microarrays, PNA chips, PNA field-effect transistors and impedance detectors.

摘要翻译： 本发明涉及与多胺接头共轭的肽核酸（PNA）及其制备方法及其应用。 更具体地说，该方法的特征在于在PNA末端连续连接具有多胺官能团的单体，并有效地将与多胺接头共轭的PNA固定在固体表面上。 使用与多胺接头共轭的PNA制备的PNA阵列与使用仅具有一个胺基团的PNA探针的PNA阵列相比，显示出用于检测靶核酸的信号的改善的灵敏度和特异性。 与多胺接头结合的PNA可用于核酸检测装置或用于基因诊断的试剂盒，例如PNA微阵列，PNA芯片，PNA场效应晶体管和阻抗检测器。

公开(公告)号：US20080233557A1

公开(公告)日：2008-09-25

申请号：US11860983

申请日：2007-09-25

申请人： Hee Kyung PARK ,  Hyunil LEE ,  Jae Jin CHOI ,  Serka KIM

发明人： Hee Kyung PARK ,  Hyunil LEE ,  Jae Jin CHOI ,  Serka KIM

IPC分类号： C12Q1/70 ,  C07H21/04

CPC分类号： C12Q1/706 ,  C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/156

摘要： Disclosed are peptide nucleic acid (PNA) probes to detect lamivudine resistant mutants of hepatitis B virus (HBV), which causes acute and chronic hepatitis, kits for detecting lamivudine resistant HBV comprising the PNA probes, and methods for detecting lamivudine resistant HBV by using the kits. They can accurately detect mutations of rtL180M, rtM204V, rtM204I and rtV2071 within B and C domains of HBV DNA polymerase gene, the main cause of lamivudine resistance, as well as mixed mutants of more than one mutant. They can detect lamivudine resistant HBV with high specificity and sensitivity.

摘要翻译： 公开了用于检测引起急性和慢性肝炎的乙型肝炎病毒（HBV）的拉米夫定抗性突变体的肽核酸（PNA）探针，用于检测包含PNA探针的拉米夫定耐药性HBV的试剂盒，以及通过使用 工具包 他们可以准确检测HBV DNA聚合酶基因的B和C结构域中rtL180M，rtM204V，rtM204I和rtV2071的突变，这是拉米夫定耐药的主要原因，以及多个突变体的混合突变体。 他们可以检测出具有高特异性和灵敏度的拉米夫定耐药HBV。