公开(公告)号：US20070264710A1

公开(公告)日：2007-11-15

申请号：US10573359

申请日：2003-10-09

申请人： Hyea-Kyung Ahn ,  Joo-Young Chung ,  Young-Ju Cho ,  Tae-Soo Kim ,  Yeo-Wook Koh ,  Seung-Wook Lim ,  Yeon-Joo Hong

发明人： Hyea-Kyung Ahn ,  Joo-Young Chung ,  Young-Ju Cho ,  Tae-Soo Kim ,  Yeo-Wook Koh ,  Seung-Wook Lim ,  Yeon-Joo Hong

IPC分类号： C07K1/00 ,  C12N5/06

CPC分类号： C07K14/524

摘要： Disclosed is a process for producing a human thrombopoietin (hTPO)-containing culture fluid, comprising the step of culturing a eukaryotic cell expressing hTPO in a serum-free medium that contains a negligible amount of serum. In addition, the present invention discloses a process for purifying hTPO from a hTPO-containing biological fluid, comprising the steps of (a) subjecting the biological fluid to affinity chromatography; (b) subjecting an eluate obtained at step (a) to hydrophobic interaction chromatography; (c) subjecting an eluate obtained at step (b) to reverse phased chromatography; and (d) subjecting an eluate obtained at step (c) to anion exchange chromatography. In addition, the present invention discloses hTPO with a high sialic acid content obtained by the process which comprises the step of loading, the eluate obtained at step (c) onto an ionic exchange chromatography column and collecting hTPO with a high content of sialic acid eluted selectively from the column by a 0.15-0.3M sodium chloride gradient.

摘要翻译： 公开了一种生产含有血小板生成素（hTPO）的培养液的方法，包括在含有可忽略量的血清的无血清培养基中培养表达hTPO的真核细胞的步骤。 另外，本发明公开了一种从含hTPO的生物流体中纯化hTPO的方法，包括以下步骤：（a）使生物液体进行亲和层析; （b）使步骤（a）获得的洗脱液进行疏水相互作用层析; （c）使步骤（b）获得的洗脱液反相定相; 和（d）使步骤（c）获得的洗脱液进行阴离子交换层析。 此外，本发明公开了通过该方法获得的具有高唾液酸含量的hTPO，其包括将步骤（c）获得的洗脱液装载到离子交换色谱柱上并收集高含量的唾液酸洗脱的hTPO 选择性地从柱中加入0.15-0.3M氯化钠梯度。

公开(公告)号：US07037491B2

公开(公告)日：2006-05-02

申请号：US11043387

申请日：2005-01-26

申请人： Joo Young Chung ,  Sang Kyu Park ,  Sang Myoung Ju ,  Hyea Kyung Ahn ,  Seung Wook Lim ,  Woo Ik Chang ,  Seung Kook Park ,  Yeo Wook Koh ,  Ji Soo Park

发明人： Joo Young Chung ,  Sang Kyu Park ,  Sang Myoung Ju ,  Hyea Kyung Ahn ,  Seung Wook Lim ,  Woo Ik Chang ,  Seung Kook Park ,  Yeo Wook Koh ,  Ji Soo Park

IPC分类号： C07K14/52

CPC分类号： C07K14/524

摘要： The present invention relates to novel human thrombopoietin (hTPO) derivatives, and to process of preparation thereof. Particularly, sugar chains are introduced into native hTPO by substituting amino acids such as asparagine for amino acids at specific positions in native hTPO, preparing novel hTPO derivatives with high activities enhancing the platelet production in vivo. Therefore, the novel hTPO derivatives of this invention may be useful for the treatment of thrombocytopenia associated with anticancer therapy or the transplantation of bone marrow.

摘要翻译： 本发明涉及新型人血小板生成素（hTPO）衍生物及其制备方法。 特别地，通过用氨基酸例如天门冬酰胺在天然hTPO中的特定位置取代氨基酸，将糖链引入天然hTPO，制备具有增强体内血小板产生的高活性的新型hTPO衍生物。 因此，本发明的新型hTPO衍生物可用于治疗与抗癌治疗或骨髓移植相关的血小板减少症。

公开(公告)号：US20050186603A1

公开(公告)日：2005-08-25

申请号：US11043387

申请日：2005-01-26

申请人： Joo Chung ,  Sang Kyu Park ,  Sang Myoung Ju ,  Hyea Ahn ,  Seung Wook Lim ,  Woo Ik Chang ,  Seung Kook Park ,  Yeo Wook Koh ,  Ji Soo Park

发明人： Joo Chung ,  Sang Kyu Park ,  Sang Myoung Ju ,  Hyea Ahn ,  Seung Wook Lim ,  Woo Ik Chang ,  Seung Kook Park ,  Yeo Wook Koh ,  Ji Soo Park

IPC分类号： C07K14/52 ,  C12Q1/68 ,  A61K38/18 ,  C07K14/475

CPC分类号： C07K14/524

摘要： The present invention relates to novel human thrombopoietin (hTPO) derivatives, and to process of preparation thereof. Particularly, sugar chains are introduced into native hTPO by substituting amino acids such as asparagine for amino acids at specific positions in native hTPO, preparing novel hTPO derivatives with high activities enhancing the platelet production in vivo. Therefore, the novel hTPO derivatives of this invention may be useful for the treatment of thrombocytopenia associated with anticancer therapy or the transplantation of bone marrow.

摘要翻译： 本发明涉及新型人血小板生成素（hTPO）衍生物及其制备方法。 特别地，通过用氨基酸例如天门冬酰胺在天然hTPO中的特定位置取代氨基酸，将糖链引入天然hTPO，制备具有增强体内血小板产生的高活性的新型hTPO衍生物。 因此，本发明的新型hTPO衍生物可用于治疗与抗癌治疗或骨髓移植相关的血小板减少症。

公开(公告)号：US06531255B2

公开(公告)日：2003-03-11

申请号：US09860960

申请日：2001-05-18

申请人： Chul-Hwan Kim ,  Seung-Wook Lim ,  Tae-Ho Park ,  Hyun-Nam Yoon

发明人： Chul-Hwan Kim ,  Seung-Wook Lim ,  Tae-Ho Park ,  Hyun-Nam Yoon

IPC分类号： G03G908

CPC分类号： G03G9/08708 ,  G03G9/081 ,  G03G9/087 ,  G03G9/08755 ,  G03G9/08795 ,  G03G9/08797 ,  Y10T428/2982

摘要： A particulate composition suitable for making color toner includes resin particles optionally containing a charge control agent. The particles are characterized by a micro-serrated surface exhibiting a surface roughness index of at least about 1.2 and preferably higher. A process for making the particulate composition utilizes a vaporizable plasticizer.

公开(公告)号：US06491904B1

公开(公告)日：2002-12-10

申请号：US09623559

申请日：2000-09-06

申请人： Seung Yeob Lee ,  Jin Seok Kang ,  Jeom Soon Shim ,  Seung Wook Lim ,  Seung Hee Han ,  Byoung Kwang Lee ,  Young Hyo Yu ,  Jong Keun Chung

发明人： Seung Yeob Lee ,  Jin Seok Kang ,  Jeom Soon Shim ,  Seung Wook Lim ,  Seung Hee Han ,  Byoung Kwang Lee ,  Young Hyo Yu ,  Jong Keun Chung

IPC分类号： A61K3174

CPC分类号： A61K38/1808

摘要： The present invention relates to a topical composition containing human epidermal growth factor (hEGF) and polyoxyethylene-polyoxypropylene copolymer having a viscosity of 4-10 cps at 37° C, 60 rpm as the base for topical formulation to improve the wound healing ability of hEGF.

摘要翻译： 本发明涉及含有人表皮生长因子（hEGF）和聚氧乙烯 - 聚氧丙烯共聚物的局部组合物，其在37℃，60rpm下的粘度为4-10cps，作为局部制剂的基础，以改善hEGF的伤口愈合能力 。

公开(公告)号：US06461783B1

公开(公告)日：2002-10-08

申请号：US09860959

申请日：2001-05-18

申请人： Chul-Hwan Kim ,  Seung-Wook Lim ,  Jongkwan Kim ,  Hyun-Nam Yoon

发明人： Chul-Hwan Kim ,  Seung-Wook Lim ,  Jongkwan Kim ,  Hyun-Nam Yoon

IPC分类号： G03G908

CPC分类号： G03G9/0804

摘要： A particulate toner composition is provided with toner resin particles containing a resin component, a colorant component, and optionally a charge control agent characterized in that the toner resin particles have a micro-serrated surface exhibiting a surface roughness index of greater than about 1.2. The novel toner particles are produced by employing a vaporizable plasticizer which is flashed off during processing.

摘要翻译： 颗粒状调色剂组合物具有包含树脂组分，着色剂组分和任选的电荷控制剂的调色剂树脂颗粒，其特征在于调色剂树脂颗粒具有表现出大于约1.2的表面粗糙度指数的微锯齿状表面。 新型调色剂颗粒通过使用在加工过程中闪蒸的可蒸发的增塑剂来制备。