公开(公告)号：US4615980A

公开(公告)日：1986-10-07

申请号：US575848

申请日：1984-02-01

申请人： Shuichi Aiba ,  Tetsuo Ohnuki

发明人： Shuichi Aiba ,  Tetsuo Ohnuki

IPC分类号： C12N15/09 ,  C12N15/76 ,  C12R1/465 ,  C12N1/00 ,  C12N15/00

CPC分类号： C12N15/76

摘要： A plasmid pOA15 in isolated form having the property of conferring pock-forming ability on Streptomyces griseus (ATCC 10137) and a molecular length of about 10 kb, and showing such sensitivity that(a) it has one restriction site recognized by BamHI,(b) it has one restriction site recognized by EcoRI,(c) it has 7 restriction sites recognized by SalI, and(d) it is not cleaved by BglII, HindIII, KpnI, PstI and XbaI,and a biologically pure culture of a microorganism containing the above plasmid.

摘要翻译： 分离形式的质粒pOA15具有赋予灰链质链霉菌（ATCC 10137）和分子长度约10kb的成熟能力的性质，并显示出（a）其具有由BamHI识别的一个限制性位点（b ）它具有EcoRI识别的一个限制性位点，（c）其具有由SalI识别的7个限制性位点，（d）其不被BglII，HindIII，KpnI，PstI和XbaI切割，并且含有微生物的生物学纯培养物 上述质粒。

公开(公告)号：US4695546A

公开(公告)日：1987-09-22

申请号：US356150

申请日：1982-03-08

申请人： Shuichi Aiba ,  Tadayuki Imanaka

发明人： Shuichi Aiba ,  Tadayuki Imanaka

IPC分类号： C12N9/28 ,  C12N9/86 ,  C12N15/75 ,  C12N15/00

CPC分类号： C12N9/2417 ,  C12N15/75 ,  C12N9/86

摘要： A process for transformation of a microorganism by introduction of a plasmid therein, the microorganism being thermophilic and gram-positive or gram-variable, and vector plasmids used therefor.

摘要翻译： 通过在其中引入质粒转化微生物的方法，微生物是嗜热的，革兰氏阳性的或革兰氏阳性的，以及用于其的载体质粒。

公开(公告)号：US5118625A

公开(公告)日：1992-06-02

申请号：US285032

申请日：1988-12-16

申请人： Shuichi Aiba ,  Tomohisa Nagasaki ,  Kohji Uchida

发明人： Shuichi Aiba ,  Tomohisa Nagasaki ,  Kohji Uchida

IPC分类号： C12N15/09 ,  C12N1/19 ,  C12N15/00 ,  C12N15/68 ,  C12N15/81 ,  C12R1/645

CPC分类号： C12N15/815 ,  C12N15/68 ,  Y10S435/911

摘要： The plasmid of the present invention contains Leu 2 gene (.beta.-isopropylmaleate dehydrogenase gene) from Saccharomycopsis lipolytica and an autonomously replicating sequence (hereafter referred to as ARS) and can be a shuttle vector between Escherichia coli and Saccharomycopsis lipolytica since it is produced from shuttle vector pVC 1 of Escherichia coli and Saccharomyces cerevisiae. An exogenous useful gene is inserted into the plasmid of the present invention, namely, shuttle vector to transform Escherichia coli, whereby a large quantity of plasmids are obtained. It becomes possible to produce a useful substance in Saccharomycopsis lipolytica as a host using the plasmid.