公开(公告)号：US20120121544A1

公开(公告)日：2012-05-17

申请号：US13125643

申请日：2009-09-28

申请人： Inpyo Choi ,  Suk-Ran Yoon ,  Soo Yun Lee ,  Joo-Yong Lee ,  Jaeseung Lim

发明人： Inpyo Choi ,  Suk-Ran Yoon ,  Soo Yun Lee ,  Joo-Yong Lee ,  Jaeseung Lim

IPC分类号： A61K35/12 ,  A61P35/00 ,  C12N5/02

CPC分类号： C12N5/0646 ,  C12N2501/2302 ,  C12N2501/2315 ,  C12N2501/2321

摘要： The present invention relates to a method for efficiently proliferating and differentiating natural killer cells (NK cells) from umbilical cord blood, more precisely a method for efficiently proliferating and differentiating natural killer cells from umbilical cord blood comprising the following steps: 1) preparing CD3 negative cells by eliminating CD3 positive T-cells from umbilical cord blood derived mononuclear cells; and 2) culturing the CD3 negative cells after treating various cytokines thereto. The present invention is advantageous in obtaining high purity NK cells in a short period of time by inducing NK cells from CD3 negative cells, compared with the conventional method inducing NK cells from haematopoietic stem cells, and in promoting NK cell proliferation and differentiation by treating different cytokines together, for example by the co-treatment of IL-15 and IL-21. That is, the method of the present invention can induce NK cells with increased anti-cancer cytotoxicity, so that it can be effectively used for anti-cancer cell therapy.

摘要翻译： 本发明涉及一种有效增殖和分化脐带血自然杀伤细胞（NK细胞）的方法，更确切地说，一种有效增殖和分化脐带血自然杀伤细胞的方法，包括以下步骤：1）制备CD3阴性 通过从脐带血衍生的单核细胞中消除CD3阳性T细胞的细胞; 和2）在处理各种细胞因子后培养CD3阴性细胞。 本发明与从造血干细胞诱导NK细胞的常规方法相比，通过诱导CD3阴性细胞的NK细胞在短时间内获得高纯度NK细胞是有利的，并且通过治疗不同的方法促进NK细胞增殖和分化 细胞因子在一起，例如通过共同治疗IL-15和IL-21。 也就是说，本发明的方法可以诱导具有增加的抗癌细胞毒性的NK细胞，从而可以有效地用于抗癌细胞治疗。