Method for Efficiently Proliferating and Differentiating Natural Killer Cells from Umbilical Cord Blood
    1.
    发明申请
    Method for Efficiently Proliferating and Differentiating Natural Killer Cells from Umbilical Cord Blood 审中-公开
    有效增殖和分化天然杀伤细胞与脐带血的方法

    公开(公告)号:US20120121544A1

    公开(公告)日:2012-05-17

    申请号:US13125643

    申请日:2009-09-28

    IPC分类号: A61K35/12 A61P35/00 C12N5/02

    摘要: The present invention relates to a method for efficiently proliferating and differentiating natural killer cells (NK cells) from umbilical cord blood, more precisely a method for efficiently proliferating and differentiating natural killer cells from umbilical cord blood comprising the following steps: 1) preparing CD3 negative cells by eliminating CD3 positive T-cells from umbilical cord blood derived mononuclear cells; and 2) culturing the CD3 negative cells after treating various cytokines thereto. The present invention is advantageous in obtaining high purity NK cells in a short period of time by inducing NK cells from CD3 negative cells, compared with the conventional method inducing NK cells from haematopoietic stem cells, and in promoting NK cell proliferation and differentiation by treating different cytokines together, for example by the co-treatment of IL-15 and IL-21. That is, the method of the present invention can induce NK cells with increased anti-cancer cytotoxicity, so that it can be effectively used for anti-cancer cell therapy.

    摘要翻译: 本发明涉及一种有效增殖和分化脐带血自然杀伤细胞(NK细胞)的方法,更确切地说,一种有效增殖和分化脐带血自然杀伤细胞的方法,包括以下步骤:1)制备CD3阴性 通过从脐带血衍生的单核细胞中消除CD3阳性T细胞的细胞; 和2)在处理各种细胞因子后培养CD3阴性细胞。 本发明与从造血干细胞诱导NK细胞的常规方法相比,通过诱导CD3阴性细胞的NK细胞在短时间内获得高纯度NK细胞是有利的,并且通过治疗不同的方法促进NK细胞增殖和分化 细胞因子在一起,例如通过共同治疗IL-15和IL-21。 也就是说,本发明的方法可以诱导具有增加的抗癌细胞毒性的NK细胞,从而可以有效地用于抗癌细胞治疗。

    Method of controlling volume of reproducing apparatus and reproducing apparatus using the same
    3.
    发明授权
    Method of controlling volume of reproducing apparatus and reproducing apparatus using the same 有权
    控制再生装置的体积的方法及使用其的再现装置

    公开(公告)号:US07925032B2

    公开(公告)日:2011-04-12

    申请号:US11609434

    申请日:2006-12-12

    申请人: Joo-Yong Lee

    发明人: Joo-Yong Lee

    IPC分类号: H03G7/00

    CPC分类号: H03G3/3005 H03G3/001

    摘要: The present invention relates to a method of controlling a volume of a reproducing apparatus and a reproducing apparatus using the same, wherein a reproduction volume of an audio file can be automatically controlled in accordance with information on a volume level of the audio file. The reproducing apparatus of the present invention includes a memory for storing at least one audio file and volume level information for controlling a reproduction volume level of the audio file, and a control unit for controlling a reproduction volume of the audio file in accordance with the volume level information recorded corresponding to the audio file when the audio file stored in the memory is reproduced.

    摘要翻译: 本发明涉及一种控制再现装置的音量的方法和使用该再现装置的再现装置,其中音频文件的再现音量可以根据关于音频文件的音量级别的信息被自动控制。 本发明的再现装置包括一个用于存储至少一个音频文件的存储器和用于控制音频文件的再现音量的音量信息,以及用于根据音量来控制音频文件的再现音量的控制单元 当再现存储在存储器中的音频文件时,对应于音频文件记录的级别信息。

    Diamond field emitter and fabrication method thereof
    4.
    发明授权
    Diamond field emitter and fabrication method thereof 失效
    金刚石场发射体及其制造方法

    公开(公告)号:US06379568B1

    公开(公告)日:2002-04-30

    申请号:US09570958

    申请日:2000-05-15

    IPC分类号: H01J1900

    CPC分类号: H01J9/025 H01J2201/30457

    摘要: A diamond field emitter and a fabrication method thereof, in which a pretreatment is performed on a surface of an Si substrate in order that diamond nuclei are uniformly formed on the Si substrate during a diamond deposition, an oxide film such as an SiO2 film is deposited on the pretreated surface of the Si substrate and removed after an etching process so that diamond powder can be selectively remained during the etching process, thus the effect of the surface pretreatment of the Si substrate remains in the selected portion during the etching process, and it is also possible to uniformly deposit the diamond in said portion. According to the present invention, the diamond field emitter having excellent and uniform field emission characteristic can be manufactured because the field emission is easily achieved at a tip shaped field emission section, and, moreover, the diamond placed on an upper end portion of the tip increases electron emission effect.

    摘要翻译: 一种金刚石场发射器及其制造方法,其中在金刚石沉积中在Si衬底的表面上进行预处理以使金刚石核均匀地形成在Si衬底上,沉积诸如SiO 2膜的氧化物膜 在Si衬底的预处理表面上,并且在蚀刻工艺之后去除,使得在蚀刻工艺期间可以选择性地保留金刚石粉末,因此在蚀刻工艺期间,Si衬底的表面预处理的效果保留在所选择的部分中,并且 也可以将金刚石均匀地沉积在所述部分中。 根据本发明,可以制造具有优异均匀的场发射特性的金刚石场发射器,因为在尖端形状的场致发射部分容易实现场致发射,此外,金刚石放置在尖端的上端部 增加电子发射效应。

    METHOD OF CONTROLLING VOLUME OF REPRODUCING APPARATUS AND REPRODUCING APPARATUS USING THE SAME
    5.
    发明申请
    METHOD OF CONTROLLING VOLUME OF REPRODUCING APPARATUS AND REPRODUCING APPARATUS USING THE SAME 有权
    使用该方法控制重现装置的体积和再生装置的方法

    公开(公告)号:US20070172084A1

    公开(公告)日:2007-07-26

    申请号:US11609434

    申请日:2006-12-12

    申请人: Joo-Yong Lee

    发明人: Joo-Yong Lee

    IPC分类号: H03G3/00

    CPC分类号: H03G3/3005 H03G3/001

    摘要: The present invention relates to a method of controlling a volume of a reproducing apparatus and a reproducing apparatus using the same, wherein a reproduction volume of an audio file can be automatically controlled in accordance with information on a volume level of the audio file. The reproducing apparatus of the present invention includes a memory for storing at least one audio file and volume level information for controlling a reproduction volume level of the audio file, and a control unit for controlling a reproduction volume of the audio file in accordance with the volume level information recorded corresponding to the audio file when the audio file stored in the memory is reproduced.

    摘要翻译: 本发明涉及一种控制再现装置的音量的方法和使用该再现装置的再现装置,其中音频文件的再现音量可以根据关于音频文件的音量级别的信息被自动控制。 本发明的再现装置包括一个用于存储至少一个音频文件的存储器和用于控制音频文件的再现音量的音量信息,以及用于根据音量来控制音频文件的再现音量的控制单元 当再现存储在存储器中的音频文件时,对应于音频文件记录的级别信息。