公开(公告)号：US20060035810A1

公开(公告)日：2006-02-16

申请号：US10508363

申请日：2003-03-18

申请人： Stephen Shears ,  Barry Potter ,  Andrew Riley

发明人： Stephen Shears ,  Barry Potter ,  Andrew Riley

IPC分类号： A61K31/66

CPC分类号： A61K31/66 ,  A61K48/00 ,  C12N9/16

摘要： Provided is a method of increasing 3,4,5,6-tetrakisphosphate by increasing the activity of inositol 1,3,4,5,6 pentakisphosphate 1-phosphatase, and a method of decreasing 3,4,5,6-tetrakisphosphate by decreasing the activity of inositol 1,3,4,5,6 pentakisphosphate 1-phosphatase. A method of reducing salt, fluid or mucous secretion in a subject, comprising increasing the activity of inositol 1,3,4,5,6 pentakisphosphate 1-phosphatase in the subject is provided. A method of treating a disease that is exacerbated by salt, fluid or mucous secretion is also provided, comprising increasing the activity of inositol 1,3,4,5,6 pentakisphosphate phosphatase in a subject having a disease that is exacerbated by mucous, whereby mucous secretion is reduced and the disease is treated. Also provided is method of increasing salt and fluid secretion in a subject, comprising decreasing the activity of inositol 1,3,4,5,6 pentakisphosphate 1-phosphatase in the subject. A method of treating a disease that is treated by increased salt and fluid secretion is provided, comprising decreasing the activity of inositol 1,3,4,5,6 pentakisphosphate 1-phosphatase in a subject having a disease that is treated by increased salt and fluid secretion, whereby salt and fluid secretion is increased and the disease is treated.

摘要翻译： 提供了通过增加肌醇1,3,4,5,6五磷酸酯1-磷酸酶的活性来增加3,4,5,6-四磷酸酯的方法，以及通过以下方法减少3,4,5,6-四磷酸酯 降低肌醇1,3,4,5,6五磷酸1-磷酸酶的活性。 提供了减少受试者中盐，液体或粘液分泌的方法，其包括增加受试者中肌醇1,3,4,5,6五磷酸酯1-磷酸酶的活性。 还提供了治疗由盐，流体或粘液分泌加重的疾病的方法，其包括增加具有由粘液加重的疾病的受试者中的肌醇1,3,4,5,6五磷酸磷酸酶的活性，由此 粘液分泌减少，疾病得到治疗。 还提供了增加受试者中盐和液体分泌的方法，包括降低受试者中肌醇1,3,4,5,6五磷酸酯1-磷酸酶的活性。 提供了治疗通过增加的盐和液体分泌物治疗的疾病的方法，其包括降低具有通过增加的盐治疗的疾病的受试者中的肌醇1,3,4,5,6五磷酸酯1-磷酸酶的活性，以及 流体分泌，从而盐和液体分泌增加并且治疗疾病。

公开(公告)号：US07351580B2

公开(公告)日：2008-04-01

申请号：US10343339

申请日：2001-08-13

申请人： Stephen Shears ,  Paul R. Reynolds ,  James N. Petitte

发明人： Stephen Shears ,  Paul R. Reynolds ,  James N. Petitte

IPC分类号： C12N5/00

CPC分类号： C12N15/8509 ,  A01K2217/05 ,  A01K2227/108 ,  A01K2227/30 ,  A01K2267/02 ,  C12N9/16

摘要： The present invention provides an isolated animal cell comprising an exogenous nucleic acid encoding a mutated phytase, wherein the cell produces phytase and secretes the phytase from the cell. The present invention also provides an animal having a phenotype not naturally occurring, characterized by secretion of phytase into the lumen of the gastrointestinal tract of the animal, the phenotype being conferred by a transgene contained in the cells of the animal, the transgene comprising a nucleic acid sequence encoding phytase and methods of making said animal.

摘要翻译： 本发明提供了包含编码突变植酸酶的外源核酸的分离的动物细胞，其中所述细胞产生植酸酶并从细胞分泌植酸酶。 本发明还提供了一种具有不是天然存在的表型的动物，其特征在于将植酸酶分泌到动物的胃肠道的内腔中，该表型由动物细胞中包含的转基因赋予，转基因包含核酸 编码植酸酶的酸序列和制备所述动物的方法。