公开(公告)号：US4940666A

公开(公告)日：1990-07-10

申请号：US939579

申请日：1986-12-09

申请人： Stephen T. Boyce ,  Richard G. Ham

发明人： Stephen T. Boyce ,  Richard G. Ham

IPC分类号： C12N5/071

CPC分类号： C12N5/0629 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/392

摘要： Disclosed are novel methods and materials for generating in vitro cultured populations of human epidermal keratinocyte cells having a characteristic colony-forming efficiency of greater than 20%. Novel media preparations and procedures are disclosed that permit isolation, serum-free primary culture and serum-free serial subculture of human epidermal keratinocytes. Also disclosed are procedures and products employing keratinocyte cells grown in serum-free conditions for medical application. i.e., skin grafting.

摘要翻译： 公开了用于产生具有大于20％的特征性集落形成效率的体外培养的人表皮角化细胞细胞群的新方法和材料。 公开了新型介质制备和程序，其允许分离，无血清的原代培养和人类表皮角化细胞的无血清连续传代培养。 还公开了使用在无血清条件下生长用于医学应用的角质细胞细胞的程序和产品。 即皮肤移植。