公开(公告)号：US5561044A

公开(公告)日：1996-10-01

申请号：US398305

申请日：1995-03-03

申请人： George T. Walker ,  James G. Nadeau ,  Patricia A. Spears ,  Colleen M. Nycz ,  Daryl D. Shank ,  James L. Schram ,  Stewart R. Jurgensen

发明人： George T. Walker ,  James G. Nadeau ,  Patricia A. Spears ,  Colleen M. Nycz ,  Daryl D. Shank ,  James L. Schram ,  Stewart R. Jurgensen

IPC分类号： C12N15/09 ,  C12Q1/04 ,  C12Q1/68 ,  C12R1/32 ,  C12R1/33 ,  C12P19/34

CPC分类号： C12Q1/689 ,  C12Q1/6851 ,  C12Q2600/16

摘要： Primers and methods for adapter-mediated multiplex amplification of the IS6110 insertion element of Mycobacterium tuberculosis (M.tb) and the 16S ribosomal gene of Mycobacterium tuberculosis, useful for simultaneously detecting and/or identifying species of the M. tuberculosis complex and other clinically relevant Mycobacterium species. Multiplex Strand Displacement Amplification (SDA) is used in a single amplification reaction which is capable of simultaneously identifying M. tuberculosis and providing a screen for substantially all of the clinically relevant species of Mycobacteria. Also disclosed are methods for adapter-mediated multiplex amplification of multiple target sequences and a single internal control sequence for determination of sample efficacy or quantitation of the targets. In a preferred embodiment, an internal control sequence is included in the amplification reaction and coamplified with the IS6110 and 16S target sequences as an indication of sample amplification activity or to quantitate the initial amount of target sequences in the sample.

摘要翻译： 针对结核分枝杆菌（M.tb）的IS6110插入元件和结核分枝杆菌的16S核糖体基因的适配器介导的多重扩增的引物和方法，可用于同时检测和/或鉴定结核分枝杆菌复合物和其他临床相关的物种 分枝杆菌种。 多重链位移扩增（SDA）用于能够同时鉴定结核分枝杆菌并为基本上所有临床相关分枝杆菌物种提供筛选的单一扩增反应。 还公开了用于多重靶序列的衔接子介导的多重扩增的方法和用于确定靶的样品功效或定量的单个内部控制序列的方法。 在优选的实施方案中，内部对照序列包括在扩增反应中并与IS6110和16S靶序列共扩增，作为样品扩增活性的指示或定量样品中靶序列的初始量。

公开(公告)号：US5470723A

公开(公告)日：1995-11-28

申请号：US111076

申请日：1993-08-24

申请人： George T. Walker ,  James G. Nadeau ,  Patricia A. Spears ,  Colleen M. Nycz ,  Daryl D. Shank ,  James L. Schram ,  Stewart R. Jurgensen

发明人： George T. Walker ,  James G. Nadeau ,  Patricia A. Spears ,  Colleen M. Nycz ,  Daryl D. Shank ,  James L. Schram ,  Stewart R. Jurgensen

IPC分类号： C12N15/09 ,  C12Q1/04 ,  C12Q1/68 ,  C12R1/32 ,  C12R1/33 ,  C07H15/12 ,  C07H17/00 ,  C12P19/34

CPC分类号： C12Q1/689 ,  C12Q1/6851 ,  C12Q2600/16

摘要： Primers and methods for adapter-mediated multiplex amplification of the IS6110 insertion element of Mycobacterium tuberculosis (M.tb) and the 16S ribosomal gene of Mycobacterium tuberculosis, useful for simultaneously detecting and/or identifying species of the M. tuberculosis complex and other clinically relevant Mycobacterium species. Multiplex Strand Displacement Amplification (SDA) is used in a single amplification reaction which is capable of simultaneously identifying M. tuberculosis and providing a screen for substantially all of the clinically relevant species of Mycobacteria. Also disclosed are methods for adapter-mediated multiplex amplification of multiple target sequences and a single internal control sequence for determination of sample efficacy or quantitation of the targets. In a preferred embodiment, an internal control sequence is included in the amplification reaction and coamplified with the IS6110 ant 16S target sequences as an indication of sample amplification activity or to quantitate the initial amount of target sequences in the sample.

摘要翻译： 针对结核分枝杆菌（M.tb）的IS6110插入元件和结核分枝杆菌的16S核糖体基因的适配器介导的多重扩增的引物和方法，可用于同时检测和/或鉴定结核分枝杆菌复合物和其他临床相关的物种 分枝杆菌种。 多重链位移扩增（SDA）用于能够同时鉴定结核分枝杆菌并为基本上所有临床相关分枝杆菌物种提供筛选的单一扩增反应。 还公开了用于多重靶序列的衔接子介导的多重扩增的方法和用于确定靶的样品功效或定量的单个内部控制序列的方法。 在优选的实施方案中，扩增反应中包含内部控制序列并与IS6110蚂蚁16S靶序列共扩增，作为样品扩增活性的指示或定量样品中靶序列的初始量。

公开(公告)号：US5188937A

公开(公告)日：1993-02-23

申请号：US733070

申请日：1991-07-17

申请人： Thomas H. Schulte ,  Stewart R. Jurgensen ,  James P. Mapes

发明人： Thomas H. Schulte ,  Stewart R. Jurgensen ,  James P. Mapes

IPC分类号： G01N33/569

CPC分类号： G01N33/56927 ,  Y10S435/975

摘要： An assay for Chlamydia includes contacting Chlamydia organisms in a liquid with a solid support having an antispecies Fe antibody immobilized thereon and an anti-Chlamydia capture antibody. After binding of Chlamydia antigen to the capture antibody and binding of the capture antibody to the antispecies antibody on the support, a tracer including a label conjugated to a signal antibody is added. After binding of the signal antibody to the antigen, the presence of Chlamydia organisms in the liquid is detected by a signal associated with the label thereby bound to the support. The invention includes a kit of materials for performing an assay according to the method of the invention.

摘要翻译： 衣原体的测定包括将液体中的衣原体生物与固定有抗病毒Fe抗体的固体支持物和抗衣原体捕获抗体接触。 在将衣原体抗原与捕获抗体结合并将捕获抗体与抗体抗体结合在载体上之后，加入包含与信号抗体缀合的标记物的示踪剂。 在信号抗体与抗原结合后，通过与标记相关联的信号检测液体中衣原体生物的存在，从而结合载体。 本发明包括用于根据本发明的方法进行测定的材料试剂盒。