公开(公告)号：US20240018596A1

公开(公告)日：2024-01-18

申请号：US17440010

申请日：2020-03-18

申请人： Stichting EuroClonality

发明人： Monika Ursula Helga Brüggemann ,  Michaela Kotrová ,  Henrik Knecht ,  Nikolaos Darzentas ,  Giovanni Cazzaniga ,  Frédéric Bernard Louis Davi ,  Jacobus Johannes Maria Van Dongen ,  Ramón Garcia-Sanz ,  David Gonzalez De Castro ,  Patricia Johanna Theodore Groenen ,  Michael Andreas Hummel ,  Elizabeth Anne Macintyre-Davi ,  Konstantinos Stamatopoulos ,  Christiane Pott ,  Jan Trka ,  Anthonie Willem Langerak

IPC分类号： C12Q1/6886 ,  C12Q1/6879

CPC分类号： C12Q1/6886 ,  C12Q1/6879 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2600/166 ,  C12Q1/6869

摘要： The invention relates to means and methods for assessing clonal immunoglobulin (IG)/T cell receptor (TR) gene rearrangements in a clinical, diagnostic and/or research setting. Provided is a quality control composition comprising a mixture of genomic DNA isolated from a set of nine cultured cell lines, said set comprising the B cell lines ALL/MIK (ALL), Raji (Burkitt lymphoma), REH (B cell precursor ALL), TMM (CML-BC/EBV+B-LCL), TOM-1 (B cell precursor ALL), WSU-NHL (B cell lymphoma) and the T cell lines JB6 (ALCL), Karpas299 (ALCL) and MOLT-13 (ALL), or wherein one or more cell lines of said set is replaced with one or more other cell line(s) comprising the same IG/TR gene rearrangements. Also provided is a quality control composition consisting of essentially equimolar amounts of genomic DNA isolated from healthy human thymus, healthy human tonsil and healthy human peripheral blood mononuclear cells.