公开(公告)号：US20240117430A1

公开(公告)日：2024-04-11

申请号：US18173060

申请日：2023-02-23

申请人： CHINESE STURGEON RESEARCH INSTITUTE OF CTG

发明人： Lei CHEN ,  Zhiyuan LI ,  Yuanjin YANG ,  Yacheng HU ,  Xin ZHU ,  Hejun DU ,  Dezhi ZHANG

IPC分类号： C12Q1/6879 ,  C12Q1/686

CPC分类号： C12Q1/6879 ,  C12Q1/686

摘要： A genome-specific gene sequence fragment of female Acipenser Dabryanus and a method for identifying the sex of Acipenser Dabryanus are disclosed. The gene sequence fragment of Acipenser Dabryanus is used to identify the sex of Acipenser Dabryanus. The method includes: performing PCR amplification on the DNA of the Acipenser Dabryanus to be tested, and judging that the Acipenser Dabryanus to be tested is female when a resultant product from the amplification shows 3 bands on an agarose gel and the Acipenser Dabryanus to be tested is male when a resultant product from the amplification shows 2 bands on an agarose gel, alternatively, judging that the Acipenser Dabryanus to be tested is female when a resultant fragment from the amplification has a 1402 bp band and it is male if there is no 1402 bp band. The gene sequence fragment is used to quickly and efficiently identify the sex of Acipenser Dabryanus.

公开(公告)号：US20240084363A1

公开(公告)日：2024-03-14

申请号：US18482273

申请日：2023-10-06

申请人： QIAGEN GmbH

发明人： Miroslav VRANES ,  Ralf PEIST ,  Mario SCHERER ,  Stefan Otto CORNELIUS ,  Margaretha KÖNIG

IPC分类号： C12Q1/6827 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q1/6879

CPC分类号： C12Q1/6827 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q1/6879 ,  C12Q2525/151 ,  C12Q2600/16 ,  C12Q2600/166

摘要： Oligonucleotide primers and probes for quantifying and/or detecting human male DNA in a forensic sample. The oligonucleotides are useful for amplifying a multicopy locus within the human Y-chromosome (MCL-Y) that shares at least 80% sequence identity to a sequence according to SEQ ID NO. 3 over a stretch of at least 60 base pairs. The oligonucleotides hybridize under stringent conditions to a nucleic acid having at least one sequence selected from the group consisting of SEQ ID NO. 3 to SEQ ID NO. 11 and/or SEQ ID NO. 17 to SEQ ID NO. 25. Kits including the oligonucleotide primers, probes and/or primer pairs and reagents for performing an amplification reaction on DNA recovered from a forensic sample are also disclosed.

公开(公告)号：US20240038339A1

公开(公告)日：2024-02-01

申请号：US18020416

申请日：2021-08-05

申请人： Myriad Women's Health, Inc.

发明人： Albert Lee ,  Kevin Haas ,  Kevin D'Auria

IPC分类号： G16B40/20 ,  G16B20/10 ,  C12Q1/6827 ,  C12Q1/6879

CPC分类号： G16B40/20 ,  G16B20/10 ,  C12Q1/6827 ,  C12Q1/6879

摘要： A method and system for analyzing sex-chromosome aneuploidies of an individual are provided. In one embodiment, a method comprises training a neural network model based on predetermined information related to at least one sex chromosome. The method also comprises determining a respective sex-chromosome status based on a normalized read depth for a gene in a genome of the individual using a machine learning algorithm. The machine learning algorithm is configured to receive, as inputs, the normalized read depth, and output the respective sex-chromosome status of the individual. In another embodiment, a system I is provided including a neural network model trained based on predetermined information related to at least one sex chromosome and is adapted to determine a respective sex-chromosome status based on a normalized read depth for a gene in a genome of the individual using a machine learning algorithm.

公开(公告)号：US20230357848A1

公开(公告)日：2023-11-09

申请号：US18149681

申请日：2023-01-04

申请人： Suzhou Fishseeds Bio-Technology Co., Ltd.

发明人： Jia DU ,  Qinghua LIU

IPC分类号： C12Q1/6879

CPC分类号： C12Q1/6879 ,  C12Q2600/158

摘要： The disclosure provides a primer pair for identifying a gene segment of a male-specific molecular marker of American shad (Alosa sapidissima). The gene segment has a nucleic acid sequence represented by SEQ ID NO: 2, and the primer pair has nucleic acid sequences represented by SEQ ID NO: 3 and SEQ ID NO: 4.

公开(公告)号：US20230151436A1

公开(公告)日：2023-05-18

申请号：US17968155

申请日：2022-10-18

申请人： The Chinese University of Hong Kong ,  GRAIL, Inc.

发明人： Yuk-Ming Dennis Lo ,  Rossa Wai Kwun Chiu ,  Kwan Chee Chan ,  Peiyong Jiang ,  Wai Kei Lam

IPC分类号： C12Q1/6888 ,  C12Q1/6879 ,  C12Q1/6806 ,  C12Q1/6886 ,  C12Q1/686 ,  G16B20/00

CPC分类号： C12Q1/6888 ,  C12Q1/6879 ,  C12Q1/6806 ,  C12Q1/6886 ,  C12Q1/686 ,  G16B20/00 ,  G16H10/40

摘要： Various embodiments are directed to applications (e.g., classification of biological samples) of the analysis of the count, the fragmentation patterns, and size of cell-free nucleic acids, e.g., plasma DNA and serum DNA, including nucleic acids from pathogens, such as viruses. Embodiments of one application can determine if a subject has a particular condition. For example, a method of present disclosure can determine if a subject has cancer or a tumor, or other pathology. Embodiments of another application can be used to assess the stage of a condition, or the progression of a condition over time. For example, a method of the present disclosure may be used to determine a stage of cancer in a subject, or the progression of cancer in a subject over time (e.g., using samples obtained from a subject at different times).

公开(公告)号：US10889861B2

公开(公告)日：2021-01-12

申请号：US15736296

申请日：2016-06-17

申请人： THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE

发明人： Matthew Huentelman

IPC分类号： C12Q1/6883 ,  C12Q1/6869 ,  C12Q1/6879 ,  C12Q1/6876 ,  C12Q1/6809

摘要： The present invention relates to a method of creating a biomarker profile, the method comprising the steps of: obtaining a sample of biofluid from a subject, wherein the sample is stored on a sample collection apparatus; removing the sample from the sample collection apparatus; extracting nucleic acids from the sample; sequencing the extracted nucleic acids to generate sequence data; and analyzing the sequence data using a two-step analytical methodology to create the biomarker profile. The present invention athletic performance in a subject.

公开(公告)号：US20200255892A1

公开(公告)日：2020-08-13

申请号：US16799810

申请日：2020-02-24

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Christina Bormann Chung ,  Julio Mulero ,  Lori Hennessy

IPC分类号： C12Q1/6858 ,  C12Q1/6879 ,  C12Q1/6888

摘要： Novel Y-STR multiplex analysis designs, primer design, allelic ladders, methods of use and kits are disclosed, including the use of primer sets designed to provide amplicons for at least 11 Y-STR loci having a base pair size of less than about 220 bp, as well as the use of primer sets designed to provide amplicons for at least 22 Y-STR loci including at least 5 rapidly mutating loci.

公开(公告)号：US20200149063A1

公开(公告)日：2020-05-14

申请号：US16729921

申请日：2019-12-30

申请人： EGGXYT LTD.

发明人： Daniel OFFEN ,  Yehuda ELRAM

IPC分类号： C12N15/85 ,  C12Q1/6879 ,  C12Q1/6897 ,  A01K67/027

摘要： The present invention, in some embodiments thereof, is directed to a method for selecting a gender of an avian fertilized unhatched egg obtained from the crossing of a transgenic male avian subject and a transgenic female avian subject. Further provided is a kit for preparing a transgenic avian subject.

公开(公告)号：US20190029236A1

公开(公告)日：2019-01-31

申请号：US15996045

申请日：2018-06-01

申请人： EGGXYT LTD

发明人： Daniel OFFEN

IPC分类号： A01K67/027 ,  C12N15/10 ,  C12Q1/6879 ,  C12N15/90 ,  C12N9/02

摘要： The present invention relates to methods of gender determination and identification in avian subjects. More specifically, the invention provides non-invasive methods using transgenic avian animals that comprise at least one reporter gene integrated into at least one gender chromosome Z or W. The transgenic avian animals of the invention are used for gender determination and selection of embryos in unhatched avian eggs.

公开(公告)号：US20180360429A1

公开(公告)日：2018-12-20

申请号：US16102370

申请日：2018-08-13

申请人： Preprogen LLC

发明人： William H. Fleming ,  Shalom Z. Hirschman

IPC分类号： A61B10/00 ,  A61B10/02 ,  C12Q1/6879 ,  C12N5/0735 ,  A61F13/84

CPC分类号： A61B10/0048 ,  A61B10/02 ,  A61B10/0291 ,  A61B2010/0074 ,  A61F13/84 ,  A61F2013/8473 ,  C12N5/0606 ,  C12Q1/6879 ,  G01N2800/36

摘要： Methods are disclosed for non-invasively obtaining fetal cells from a pregnant female. The methods include placing an absorbent medium in an interlabial or intravaginal space or adjacent to the perineum at the vaginal opening of the pregnant female, and collecting vaginal fluid comprising cells in the absorbent medium while the absorbent medium is interlabial or intravaginal space or adjacent to the perineum at the vaginal opening. The absorbent medium is removed and cells are isolated from the absorbent medium to obtain the fetal cells. The fetal cells can be, for example, somatic cells, embroyic stem cells, fetal stem cells or trophoblast cells.