公开(公告)号：US20070254294A1

公开(公告)日：2007-11-01

申请号：US11662051

申请日：2005-03-18

申请人： Woo-Chul Moon ,  Myung-Ryurl Oh ,  Su-Bin Yim ,  Tae-Han Eum ,  Mi-Ae Lee ,  Bu-Il Jeon

发明人： Woo-Chul Moon ,  Myung-Ryurl Oh ,  Su-Bin Yim ,  Tae-Han Eum ,  Mi-Ae Lee ,  Bu-Il Jeon

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12N9/12

CPC分类号： C12Q1/6806 ,  C12N15/1003 ,  C12Q2527/125

摘要： If DNA of human, animal, plant or bacteria, or a bio-sample containing DNA such as blood is well mixed with a water-soluble chitosan, and stored as a liquid state or a solid state using a solid medium such as a paper, it is possible to store DNA stably at room temperature for a long time, and it is also useful for a gene assay henceforth. The DNA storage method is simple and economical, and a product prepared by applying the method such as a DNA card and a PCR kit can store, carry and collect multiple DNA samples, and it is also very useful for an automatic assay. In addition, the DNA ID card can play a role of DNA bank which stores personal DNAs, and it is helpful for personal identification and medical treatment by storing the personal genetic information as well.

摘要翻译： 如果人，动物，植物或细菌的DNA或含有DNA的生物样品如血液与水溶性壳聚糖充分混合，并使用诸如纸的固体培养基作为液体状态或固体状态储存， 可以长时间在室温下稳定存储DNA，此后也可用于基因测定。 DNA存储方法简单经济，通过应用DNA卡和PCR试剂盒等方法制备的产品可以存储，携带和收集多个DNA样品，对自动测定也非常有用。 此外，DNA身份证可以起到存储个人DNA的DNA库的作用，也可以通过存储个人遗传信息对个人身份识别和医疗进行处理。

公开(公告)号：US07670774B2

公开(公告)日：2010-03-02

申请号：US11664549

申请日：2005-03-18

申请人： Woo-Chul Moon ,  Myung-Ryurl Oh ,  Su-Bin Yim ,  Tae-Han Eum ,  Eun-Hae Jung ,  Jung-Eun Ko ,  Jae-Han Bae

发明人： Woo-Chul Moon ,  Myung-Ryurl Oh ,  Su-Bin Yim ,  Tae-Han Eum ,  Eun-Hae Jung ,  Jung-Eun Ko ,  Jae-Han Bae

IPC分类号： C12Q1/64 ,  C12M1/00

CPC分类号： C12Q1/708 ,  Y10S435/975 ,  C12Q2565/501 ,  C12Q2537/143 ,  C12Q2531/113

摘要： Oligonucleotide probes for analyzing 40 types of HPV were synthesized, and DNA chips were produced by using the oligonucleotide probes. The synthesis of the oligonucleotide probes is based on clones of L1 and E6/E7 genes of 35 types of HPV obtained from cervical cell specimens from 4,898 Korean adult women and tissue specimens from 68 cervical cancer cases in addition to information based on American and European cases. The DNA chips can analyze the 40 types of HPV found in cervical, diagnose complex infection by at least one type of HPV, and have excellent diagnostic sensitivity and specificity on HPV genetic type up to 100% and reproducibility. Also, the DAN chips are superior to conventional analytic method, and very economical, since they can analyze numerous specimens in shortest time. Accordingly, the DNA chips are useful for predicting cervical cancer and precancerous lesion.

摘要翻译： 合成分析40种HPV的寡核苷酸探针，并用寡核苷酸探针制备DNA芯片。 寡核苷酸探针的合成基于来自4,898名韩国成年妇女的宫颈细胞标本获得的35种HPV的L1和E6 / E7基因的克隆，以及来自68个宫颈癌病例的组织标本以及基于美国和欧洲病例的信息 。 DNA芯片可以分析宫颈发现的40种HPV，通过至少一种HPV诊断复杂感染，对HPV遗传型的诊断敏感性和特异性高达100％，具有重现性。 此外，DAN芯片优于常规分析方法，非常经济，因为它们可以在最短时间内分析多个样品。 因此，DNA芯片可用于预测子宫颈癌和癌前病变。

公开(公告)号：US20070248968A1

公开(公告)日：2007-10-25

申请号：US11664549

申请日：2005-03-18

申请人： Woo-Chul Moon ,  Myung-Ryurl Oh ,  Su-Bin Yim ,  Tae-Han Eum ,  Eun-Hae Jung ,  Jung-Eun Ko ,  Jae-Han Bae

发明人： Woo-Chul Moon ,  Myung-Ryurl Oh ,  Su-Bin Yim ,  Tae-Han Eum ,  Eun-Hae Jung ,  Jung-Eun Ko ,  Jae-Han Bae

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/708 ,  Y10S435/975 ,  C12Q2565/501 ,  C12Q2537/143 ,  C12Q2531/113

摘要： Oligonucleotide probes for analyzing 40 types of HPV were synthesized, and DNA chips were produced by using the oligonucleotide probes. The synthesis of the oligonucleotide probes is based on clones of L1 and E6/E7 genes of 35 types of HPV obtained from cervical cell specimens from 4,898 Korean adult women and tissue specimens from 68 cervical cancer cases in addition to information based on American and European cases. The DNA chips can analyze the 40 types of HPV found in cervical, diagnose complex infection by at least one type of HPV, and have excellent diagnostic sensitivity and specificity on HPV genetic type up to 100% and reproducibility. Also, the DAN chips are superior to conventional analytic method, and very economical, since they can analyze numerous specimens in shortest time. Accordingly, the DNA chips are useful for predicting cervical cancer and precancerous lesion.

摘要翻译： 合成分析40种HPV的寡核苷酸探针，并用寡核苷酸探针制备DNA芯片。 寡核苷酸探针的合成基于来自4,898名韩国成年妇女的宫颈细胞标本获得的35种HPV的L1和E6 / E7基因的克隆，以及来自68个宫颈癌病例的组织标本以及基于美国和欧洲病例的信息 。 DNA芯片可以分析宫颈发现的40种HPV，通过至少一种HPV诊断复杂感染，对HPV遗传型的诊断敏感性和特异性高达100％，具有重现性。 此外，DAN芯片优于常规分析方法，非常经济，因为它们可以在最短时间内分析多个样品。 因此，DNA芯片可用于预测子宫颈癌和癌前病变。