公开(公告)号：US20130156809A1

公开(公告)日：2013-06-20

申请号：US13811690

申请日：2011-07-28

申请人： Subash Sad ,  Lakshmi Krishnan ,  Valeria Alcon ,  Kevin G. Young ,  Fanny Tzelepis

发明人： Subash Sad ,  Lakshmi Krishnan ,  Valeria Alcon ,  Kevin G. Young ,  Fanny Tzelepis

IPC分类号： A61K39/12 ,  A61K39/00

CPC分类号： C07K14/77 ,  A61K39/0005 ,  A61K39/0011 ,  A61K39/12 ,  A61K2039/522 ,  A61K2039/523 ,  C07K14/195 ,  C07K14/4713 ,  C07K14/4748 ,  C07K14/70596 ,  C07K14/71 ,  C07K2319/036 ,  C07K2319/35 ,  C12N1/20 ,  C12N9/0059 ,  C12N9/2462 ,  C12N9/6494 ,  C12N2760/10034 ,  C12Y110/03001 ,  C12Y304/24011 ,  Y02A50/41

摘要： The present invention relates to a recombinant bacterium expressing an antigen that is translocated to the cytosol of a host organism, and uses thereof. To this end, the present invention provides a recombinant bacterium comprising a nucleic acid encoding an antigen that is translocated to the cytosol of a host cell utilizing Type III secretion system. The recombinant bacterium is generally chosen from intracellular pathogens that reside in the phagosome and fail to induce rapid T cell activation. The translocated antigen may be a viral antigen, a bacterial antigen, or a tumour antigen. Methods of imparting immunity using the recombinant bacterium are also provided.

公开(公告)号：US20080248094A1

公开(公告)日：2008-10-09

申请号：US11940858

申请日：2007-11-15

申请人： G. Dennis SPROTT ,  Lakshmi KRISHNAN ,  Subash SAD

发明人： G. Dennis SPROTT ,  Lakshmi KRISHNAN ,  Subash SAD

IPC分类号： A61K9/127 ,  A61K39/00 ,  A61P37/00

CPC分类号： A61K39/39 ,  A61K2039/55555 ,  A61K2039/55594

摘要： The invention relates to a liposome comprising a chloroform soluble and extractable total polar lipid of Mycobacterium spp, particularly a chloroform soluble extractable total polar lipid of Mycobacterium bovis BCG. The chloroform soluble and extractable polar lipid may comprise at least one of phosphatidylinositol (PI), phosphatidylinositol mannoside (PIM1), phosphatidylinositol dimannoside (PIM2), mono and dipalmitoylated forms of PIM1 and PIM2, phospholipid of 899 m/z, phosphatidylethanolamine and cardiolipid. The liposome may be prepared by drying chloroform soluble and extractable lipid and then hydrating said dried lipid at a temperature of 65 to 75° C. in water or phosphate buffered saline (PBS). The liposome may be used, for example, to activate dendritic cells to secrete cytokines and modulate an immune response in a mammal, or to direct an immune response to confer protection against a pathogen or a cancer.

摘要翻译： 本发明涉及包含分枝杆菌属的可溶于氯仿和可提取的总极性脂质的脂质体，特别是牛分枝杆菌BCG的氯仿可溶性可提取的总极性脂质。 氯仿可溶性和可萃取的极性脂质可以包含磷脂酰肌醇（PI），磷脂酰肌醇甘露糖苷（PIM 1 SUB），磷脂酰肌醇二甘草苷（PIM 2 N 2），单和二棕榈酰化形式 的PIM 1和PIM 2 2，磷脂899m / z，磷脂酰乙醇胺和心磷脂。 脂质体可以通过干燥氯仿可溶性和可提取的脂质，然后在65至75℃的温度下在水或磷酸盐缓冲盐水（PBS）中水合所述干燥的脂质来制备。 脂质体可以用于例如激活树突细胞以分泌细胞因子并调节哺乳动物的免疫应答，或引导免疫应答以赋予对病原体或癌症的保护。