Mutant beetle luciferase, gene, recombinant vector, transformant, and method for preparing mutant beetle luciferase

    公开(公告)号:US10851141B2

    公开(公告)日:2020-12-01

    申请号:US16311301

    申请日:2017-06-19

    Abstract: The present invention provides a mutant beetle luciferase and the like, having mutation in which the amino acid corresponding to valine at position 288 in the amino acid sequence of wild-type Photinus pyralis luciferase is isoleucine, leucine or phenylalanine, mutation in which the amino acid corresponding to leucine at position 376 in the aforementioned sequence is proline, mutation in which the amino acid corresponding to glutamic acid at position 455 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, or mutation in which the amino acid corresponding to glutamic acid at position 488 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, in the amino acid sequence encoding a wild-type beetle luciferase, and characterized in that a luminescence intensity due to a luciferin-luciferase luminescence reaction in a 0.9% by mass NaCl solution is 50% or more of that in a NaCl-free solution.

    MUTANT BEETLE LUCIFERASE, GENE, RECOMBINANT VECTOR, TRANSFORMANT, AND METHOD FOR PREPARING MUTANT BEETLE LUCIFERASE

    公开(公告)号:US20190352350A1

    公开(公告)日:2019-11-21

    申请号:US16311301

    申请日:2017-06-19

    Abstract: The present invention provides a mutant beetle luciferase and the like, having mutation in which the amino acid corresponding to valine at position 288 in the amino acid sequence of wild-type Photinus pyralis luciferase is isoleucine, leucine or phenylalanine, mutation in which the amino acid corresponding to leucine at position 376 in the aforementioned sequence is proline, mutation in which the amino acid corresponding to glutamic acid at position 455 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, or mutation in which the amino acid corresponding to glutamic acid at position 488 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, in the amino acid sequence encoding a wild-type beetle luciferase, and characterized in that a luminescence intensity due to a luciferin-luciferase luminescence reaction in a 0.9% by mass NaCl solution is 50% or more of that in a NaCl-free solution.

    Estimating sound source position with microphone array control

    公开(公告)号:US09818430B2

    公开(公告)日:2017-11-14

    申请号:US14897407

    申请日:2013-06-11

    Abstract: Problem to be SolvedTo effectively operate a microphone array provided with a sound source position estimating function.SolutionA microphone control system of the present invention is realized by the use of a tablet-type computer, for example. On the display of the control system, a two-dimensional simulation diagram 302, for example, simulating the space in which the microphone array is disposed. An array mark 304 simulating the exterior shape of the microphone array is displayed on the simulation diagram 302. Further, a sound source mark 316 indicating the position of the sound source estimated by the microphone array is displayed on the simulation diagram 302. Thus, an operator can intuitively find the position of the sound source in the space in which the microphone array is disposed, by referring to the simulation diagram 302, the array mark 304 and the sound source mark 316 displayed on the simulation diagram. Thus, the usage of the microphone array is improved.

    Loudspeaker system
    4.
    发明授权

    公开(公告)号:US09648413B2

    公开(公告)日:2017-05-09

    申请号:US14765364

    申请日:2013-02-05

    Abstract: Provided is a loudspeaker system capable of easily determining control parameters for controlling the directional characteristics of a speaker set including two or more speaker units. The loudspeaker system is configured to include: a speaker set 11 including two or more speaker units 2; a camera 15 adapted to photograph an acoustic space of the speaker set 11; a monitor 22 adapted to display a camera image 30 photographed by the camera 15; and a directional control parameter generating part 24 adapted to generate directional control parameters providing the directional characteristics of the speaker set 11. The directional control parameter generating parts 24 includes: a target position designation part 101 adapted to, on the basis of user operations, designate target positions 32 on the camera image 30; a directional control angle calculation part 103 adapted to, on the basis of the target positions 32, obtain directional control angles θ with respect to a front direction of the speaker set 11; and a directional control parameter determination part 104 adapted to, on the basis of the directional control angles θ, determine the directional control parameters.

    CHANNEL SIMULATION DEVICE AND CHANNEL SIMULATION PROGRAM

    公开(公告)号:US20180138997A1

    公开(公告)日:2018-05-17

    申请号:US15574402

    申请日:2015-05-22

    Inventor: Katsuhiro Mori

    Abstract: This channel simulation device is provided with a memory for storing a distance calculation parameter and reception strength calculation parameter, and a processor for estimating desired-wave reception strength and interfering-wave reception strength on the basis of the distance calculation parameter and the reception strength calculation parameter. The desired-wave reception strength refers, for each set of two areas selected from a plurality of areas, to reception strength when a transmission signal transmitted from the wireless microphone of one area is received by a receiver in the one area. The interfering-wave reception strength refers to reception strength when a transmission signal transmitted from the wireless microphone of the other area is received by the receiver in the one area.

    PUBLIC ADDRESS DEVICE, HOWLING SUPPRESSION DEVICE, AND HOWLING SUPPRESSION METHOD

    公开(公告)号:US20230254640A1

    公开(公告)日:2023-08-10

    申请号:US18010842

    申请日:2020-07-09

    CPC classification number: H04R3/02 H04R3/04 H04R27/00 H04R2227/007

    Abstract: An object is to provide a public address device that effectively prevents occurrence of howling without a drop of the gain. A public address device 100 includes: a loudspeaker 1 that generates a reproduced sound on the basis of a loudspeaker driving signal u(n); a microphone 2 that collects the reproduced sound and an input sound v(n) to generate a microphone-collected-sound signal y(n); a first filter 301 that generates, on the basis of the loudspeaker driving signal u(n), a pseudo echo signal e(n); an echo-cancelling unit 302 that obtains a difference between the microphone-collected-sound signal y(n) and the pseudo echo signal e(n) to generate an echo-cancelled signal d(n); a second filter 311 that whitens the input sound v(n) included in the loudspeaker driving signal u(n); a third filter 312 that whitens the input sound v(n) included in the microphone-collected-sound signal y(n); a first adaptive filter 313 that uses, as a reference signal, an output signal output from the second filter 311, and uses, as a desired signal, an output signal output from the third filter 311, and estimates a propagation characteristic Wo from the loudspeaker 1 to the microphone 2; a unit that repeatedly updates a filter coefficient W of the first filter 301 on the basis of a filter coefficient W identified by the first adaptive filter 313; and a frequency shifting unit 32 that performs a frequency shift on the echo-cancelled signal d(n) to generate the loudspeaker driving signal u(n).

    MICROPHONE ARRAY CONTROL SYSTEM
    8.
    发明申请
    MICROPHONE ARRAY CONTROL SYSTEM 有权
    麦克风阵列控制系统

    公开(公告)号:US20160148624A1

    公开(公告)日:2016-05-26

    申请号:US14897407

    申请日:2013-06-11

    Abstract: Problem to be SolvedTo effectively operate a microphone array provided with a sound source position estimating function.SolutionA microphone control system of the present invention is realized by the use of a tablet-type computer, for example. On the display of the control system, a two-dimensional simulation diagram 302, for example, simulating the space in which the microphone array is disposed. An array mark 304 simulating the exterior shape of the microphone array is displayed on the simulation diagram 302. Further, a sound source mark 316 indicating the position of the sound source estimated by the microphone array is displayed on the simulation diagram 302. Thus, an operator can intuitively find the position of the sound source in the space in which the microphone array is disposed, by referring to the simulation diagram 302, the array mark 304 and the sound source mark 316 displayed on the simulation diagram. Thus, the usage of the microphone array is improved.

    Abstract translation: 要解决的问题为了有效地操作具有声源位置估计功能的麦克风阵列。 解决方案本发明的麦克风控制系统例如通过使用平板式计算机来实现。 在控制系统的显示器上,例如,模拟麦克风阵列设置的空间的二维模拟图302。 在模拟图302上显示模拟麦克风阵列的外形的阵列标记304.此外,在模拟图302上显示指示由麦克风阵列估计的声源的位置的声源标记316。 操作者可以通过参考模拟图表302上显示的模拟图302,阵列标记304和声源标记316直观地找到放置麦克风阵列的空间中声源的位置。 因此,提高了麦克风阵列的使用。

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