公开(公告)号：US20110163228A1

公开(公告)日：2011-07-07

申请号：US13063616

申请日：2009-09-24

Applicant: Tae Geol Lee ,  Dae Won Moon ,  Hyegeun Min

Inventor： Tae Geol Lee ,  Dae Won Moon ,  Hyegeun Min

IPC: H01J49/40 ,  G01N23/225

CPC classification number: G01N23/2255

Abstract: A quantification method of functional groups in an organic thin layer includes: a) measuring an absolute quantity per unit area of an analysis reference material having functional groups included in a reference organic thin layer by means of MEIS spectroscopy; b) carrying out spectrometry for the same reference organic thin layer as in a) and thereby obtaining peak intensities of the functional groups in the reference organic thin layer; c) carrying out the same spectrometry as in b) for an organic thin layer to be analyzed having the same functional groups and thereby measuring peak intensities of the functional groups with unknown quantity; and d) comparing the peak intensities of the functional groups measured in b) with respect to the absolute quantity of the analysis reference material in a) and thereby determining the absolute quantity per unit area of the functional groups with unknown quantity measured in c).

Abstract translation: 有机薄层中的官能团的定量方法包括：a）通过MEIS光谱测量具有包括在参考有机薄层中的官能团的分析参考材料的每单位面积的绝对量; b）进行与a）相同的参考有机薄层的光谱测定，从而获得参考有机薄层中的官能团的峰值强度; c）进行与b）相同的光谱测定，对于具有相同官能团的待分析有机薄层，从而测量具有未知量的官能团的峰强度; 和d）比较a）中测定的官能团的峰强度相对于分析参考物质的绝对量，从而确定在c）中测量的具有未知量的官能团的每单位面积的绝对量。

公开(公告)号：US08450684B2

公开(公告)日：2013-05-28

申请号：US13063616

申请日：2009-09-24

Applicant: Tae Geol Lee ,  Dae Won Moon ,  Hyegeun Min

Inventor： Tae Geol Lee ,  Dae Won Moon ,  Hyegeun Min

IPC: G01N23/203

CPC classification number: G01N23/2255

Abstract: A quantification method of functional groups in an organic thin layer includes: a) measuring an absolute quantity per unit area of an analysis reference material having functional groups included in a reference organic thin layer by means of MEIS spectroscopy; b) carrying out spectrometry for the same reference organic thin layer as in a) and thereby obtaining peak intensities of the functional groups in the reference organic thin layer; c) carrying out the same spectrometry as in b) for an organic thin layer to be analyzed having the same functional groups and thereby measuring peak intensities of the functional groups with unknown quantity; and d) comparing the peak intensities of the functional groups measured in b) with respect to the absolute quantity of the analysis reference material in a) and thereby determining the absolute quantity per unit area of the functional groups with unknown quantity measured in c).

Abstract translation: 有机薄层中的官能团的定量方法包括：a）通过MEIS光谱测量具有包括在参考有机薄层中的官能团的分析参考材料的每单位面积的绝对量; b）进行与a）相同的参考有机薄层的光谱测定，从而获得参考有机薄层中的官能团的峰值强度; c）进行与b）相同的光谱测定，对于具有相同官能团的待分析有机薄层，从而测量具有未知量的官能团的峰强度; 和d）比较a）中测定的官能团的峰强度相对于分析参考物质的绝对量，从而确定在c）中测量的具有未知量的官能团的每单位面积的绝对量。

公开(公告)号：US20130095504A1

公开(公告)日：2013-04-18

申请号：US13806203

申请日：2011-06-24

Applicant: Tae Geol Lee ,  Young-Lai Cho ,  Mi Young Son

Inventor： Tae Geol Lee ,  Young-Lai Cho ,  Mi Young Son

IPC: G01N33/574

CPC classification number: G01N33/57488 ,  G01N33/553 ,  G01N33/6848

Abstract: Provided is a detection kit, which a disease detection kit used together with a secondary ion mass spectrometer in order to detect a disease marker contained in a biological sample, the detection kit including: a base including a noble metal thin film formed thereon; a reactant containing peptide specifically reacting with the disease marker; a first storage unit filled with the reactant; a test substance containing a biological sample of a possible disease carrier; a second storage unit filled with the test substance; a mixing unit mixing the reactant and the test substance with each other to prepare a detection substance containing the specific reactant, which is the peptide specifically reacted with the disease marker contained in the biological sample; and a contact unit contacting the detection substance prepared by the mixing unit with the base to bond the specific reactant to the noble metal film of the base.

Abstract translation: 提供了一种检测试剂盒，其是与二次离子质谱仪一起使用以检测生物样品中所含的疾病标志物的疾病检测试剂盒，所述检测试剂盒包括：其上形成有贵金属薄膜的基材; 含有特异性与疾病标记物反应的肽的反应物; 填充有反应物的第一存储单元; 含有可能的疾病携带者的生物样品的测试物质; 填充有测试物质的第二存储单元; 混合单元，将反应物和测试物质相互混合，制备含有特异性反应物的检测物质，其是特异性与生物样品中所含疾病标志物反应的肽; 以及接触单元，将由所述混合单元制备的检测物质与所述基底接触，以将所述特定反应物键合到所述基底的贵金属膜。

公开(公告)号：US20110152119A1

公开(公告)日：2011-06-23

申请号：US13059445

申请日：2009-08-21

Applicant: Tae Geol Lee ,  Dae Won Moon ,  Hye geun Min

Inventor： Tae Geol Lee ,  Dae Won Moon ,  Hye geun Min

IPC: C40B30/04

CPC classification number: G01N33/54353

Abstract: A method for direct quantification of the areal density (number per surface area of a substrate) of an analyte including a biochemical substance bound on the surface of a substrate and for direct quantification of the binding efficiency of biochemical substances is disclosed. Specifically, the areal density of an analyte including a biochemical substance bound on the surface of a substrate, and the binding efficiency between a first biochemical substance fixed on the substrate surface and a second biochemical substance is measured by ion scattering spectroscopy (ISS).

Abstract translation: 公开了用于直接定量包含结合在基材表面上的生物化学物质的分析物的面密度（基底的每个表面积的数量）和用于直接定量生化物质的结合效率的方法。 具体地，通过离子散射光谱法（ISS）测量包含结合在基材表面上的生物化学物质的分析物的面密度和固定在基材表面上的第一生物化学物质与第二生化物质之间的结合效率。

公开(公告)号：US08772713B1

公开(公告)日：2014-07-08

申请号：US14117116

申请日：2012-05-03

Applicant: Jeong Hee Moon ,  Dae Won Moon ,  Tae Geol Lee ,  Sohee Yoon ,  Ju Hwang Kim

Inventor： Jeong Hee Moon ,  Dae Won Moon ,  Tae Geol Lee ,  Sohee Yoon ,  Ju Hwang Kim

IPC: H01J49/40 ,  H01J37/26 ,  H01J37/28 ,  B01D59/44 ,  H01J49/00

CPC classification number: H01J49/0004 ,  H01J49/142 ,  H01J49/164 ,  H01J49/40 ,  H01J49/406

Abstract: The present invention aims to provide a time-of-flight based mass microscope system for an ultra-high speed multi-mode mass analysis, for using a laser beam or an ion beam simultaneously to enable both a low molecular weight analysis such as for drugs/metabolome/lipids/peptides and a high molecular weight analysis such as for genes/proteins, without being limited by the molecular weight of the object being analyzed, and for significantly increasing the measuring speed by using a microscope method instead of a microprobe method.

Abstract translation: 本发明旨在提供一种用于超高速多模式质量分析的飞行时间质量显微镜系统，用于同时使用激光束或离子束，以实现低分子量分析，例如用于药物 /代谢组胺/脂质/肽和高分子量分析，例如用于基因/蛋白质，而不受被分析物体的分子量的限制，并且通过使用显微镜法而不是微探针法显着增加测量速度。

公开(公告)号：US20140183354A1

公开(公告)日：2014-07-03

申请号：US14117116

申请日：2012-05-03

Applicant: Jeong Hee Moon ,  Dae Won Moon ,  Tae Geol Lee ,  Sohee Yoon ,  Ju Hwang Kim

Inventor： Jeong Hee Moon ,  Dae Won Moon ,  Tae Geol Lee ,  Sohee Yoon ,  Ju Hwang Kim

IPC: H01J49/00 ,  H01J49/40

CPC classification number: H01J49/0004 ,  H01J49/142 ,  H01J49/164 ,  H01J49/40 ,  H01J49/406

Abstract: The present invention aims to provide a time-of-flight based mass microscope system for an ultra-high speed multi-mode mass analysis, for using a laser beam or an ion beam simultaneously to enable both a low molecular weight analysis such as for drugs/metabolome/lipids/peptides and a high molecular weight analysis such as for genes/proteins, without being limited by the molecular weight of the object being analyzed, and for significantly increasing the measuring speed by using a microscope method instead of a microprobe method.

Abstract translation: 本发明旨在提供一种用于超高速多模式质量分析的飞行时间质量显微镜系统，用于同时使用激光束或离子束，以实现低分子量分析，例如用于药物 /代谢组胺/脂质/肽和高分子量分析，例如用于基因/蛋白质，而不受被分析物体的分子量的限制，并且通过使用显微镜法而不是微探针法显着增加测量速度。

公开(公告)号：US20110101217A1

公开(公告)日：2011-05-05

申请号：US12988925

申请日：2009-04-24

Applicant: Sang Yun Han ,  Tae Geol Lee ,  Dae Won Moon

Inventor： Sang Yun Han ,  Tae Geol Lee ,  Dae Won Moon

IPC: H01J49/00

CPC classification number: G01N33/6851 ,  G01N2610/00 ,  H01J49/0418

Abstract: Disclosed is a method for carrying out matrix-free mass spectrometry, which includes subjecting an analyte sample containing a self-assembled monolayer on the surface of a substrate to laser desorption/ionization. The method for carrying out matrix-free mass spectrometry involves simple pretreatment of an analyte sample with a cationic solution without using any solid matrix to cause effective laser desorption/ionization of the analyte sample, and minimizes a biochemical and physiological change in the sample that may occur during the pretreatment of the sample. In addition, the method is applicable to quantitative analysis because it provides high reproducibility of the results by virtue of the uniform treatment with the cationic solution over the whole areas of the sample. Further, the method enables two-dimensional mapping analysis.

Abstract translation: 公开了一种进行无基质质谱的方法，其包括使含有自组装单层的分析物样品在基板的表面上进行激光解吸/电离。 用于进行无基质质谱的方法包括用阳离子溶液简单地预处理分析物样品，而不使用任何固体基质来引起分析物样品的有效激光解吸/电离，并且最小化样品中的生物化学和生理变化， 在样品的预处理期间发生。 此外，该方法适用于定量分析，因为通过在样品的整个区域上的阳离子溶液的均匀处理，提供了高结果的再现性。 此外，该方法能够进行二维映射分析。

公开(公告)号：US20110095179A1

公开(公告)日：2011-04-28

申请号：US12994263

申请日：2008-06-25

Applicant: Tae Geol Lee ,  Dae Won Moon ,  Byong Chul Yoo ,  In Hoo Kim

Inventor： Tae Geol Lee ,  Dae Won Moon ,  Byong Chul Yoo ,  In Hoo Kim

IPC: B01D59/44

CPC classification number: G01N33/6851 ,  G01N33/57446 ,  Y10T436/173845 ,  Y10T436/200833

Abstract: The present invention relates to a disease diagnosis method, a marker screening method, and a marker using a time-of-flight secondary ion mass spectrometry (TOF-SIMS), and more particularly, to a large intestine cancer diagnosis method, a large intestine cancer marker screening method, and a large intestine cancer marker using a time-of-flight secondary ion mass spectrometry (TOF-SIMS). Specifically, the present invention provides a method diagnosing a disease using a pattern of secondary ion mass (m/s) peaks of biological samples measured using a time-of- flight secondary ion mass spectrometry (TOF-SIMS) as a marker, a marker screening method being a reference judging an existence or non-existence of a disease, and a marker configured of specific secondary ion mass peaks.

Abstract translation: 本发明涉及疾病诊断方法，标记筛选方法和使用飞行时间二次离子质谱法（TOF-SIMS）的标记物，更具体地，涉及大肠癌诊断方法，大肠 癌症标志物筛选方法和使用飞行时间二次离子质谱（TOF-SIMS）的大肠癌标记物。 具体而言，本发明提供使用飞行时间二次离子质谱法（TOF-SIMS）作为标记测定的生物样品的二次离子质量峰（m / s）峰的图案来诊断疾病的方法，标记 筛选方法是判断疾病的存在或不存在的参考，以及由特定二次离子质量峰构成的标记。

公开(公告)号：US08871438B2

公开(公告)日：2014-10-28

申请号：US13570507

申请日：2012-08-09

Applicant: Tae Geol Lee ,  Dae Won Moon ,  Byong Chul Yoo ,  In Hoo Kim

Inventor： Tae Geol Lee ,  Dae Won Moon ,  Byong Chul Yoo ,  In Hoo Kim

IPC: G01N33/574 ,  G01N33/68

CPC classification number: G01N33/6851 ,  G01N33/57446 ,  Y10T436/173845 ,  Y10T436/200833

Abstract: The present invention relates to a disease diagnosis method, a marker screening method, and a marker using a time-of-flight secondary ion mass spectrometry (TOF-SIMS), and more particularly, to a large intestine cancer diagnosis method, a large intestine cancer marker screening method, and a large intestine cancer marker using a time-of-flight secondary ion mass spectrometry (TOF-SIMS). Specifically, the present invention provides a method diagnosing a disease using a pattern of secondary ion mass (m/z) peaks of biological samples measured using a time-of-flight secondary ion mass spectrometry (TOF-SIMS) as a marker, a marker screening method being a reference judging an existence or non-existence of a disease, and a marker configured of specific secondary ion mass peaks.

Abstract translation: 本发明涉及疾病诊断方法，标记筛选方法和使用飞行时间二次离子质谱法（TOF-SIMS）的标记物，更具体地，涉及大肠癌诊断方法，大肠 癌症标志物筛选方法和使用飞行时间二次离子质谱（TOF-SIMS）的大肠癌标记物。 具体地，本发明提供使用飞行时间二次离子质谱法（TOF-SIMS）作为标记测定的生物样品的二次离子质量（m / z）峰图案，标记物 筛选方法是判断疾病的存在或不存在的参考，以及由特定二次离子质量峰构成的标记。

公开(公告)号：US20130065266A1

公开(公告)日：2013-03-14

申请号：US13570507

申请日：2012-08-09

Applicant: Tae Geol Lee ,  Dae Won Moon ,  Byong Chul Yoo ,  In Hoo Kim

Inventor： Tae Geol Lee ,  Dae Won Moon ,  Byong Chul Yoo ,  In Hoo Kim

IPC: G01N27/62 ,  H01J49/40

CPC classification number: G01N33/6851 ,  G01N33/57446 ,  Y10T436/173845 ,  Y10T436/200833

Abstract: The present invention relates to a disease diagnosis method, a marker screening method, and a marker using a time-of-flight secondary ion mass spectrometry (TOF-SIMS), and more particularly, to a large intestine cancer diagnosis method, a large intestine cancer marker screening method, and a large intestine cancer marker using a time-of-flight secondary ion mass spectrometry (TOF-SIMS). Specifically, the present invention provides a method diagnosing a disease using a pattern of secondary ion mass (m/z) peaks of biological samples measured using a time-of-flight secondary ion mass spectrometry (TOF-SIMS) as a marker, a marker screening method being a reference judging an existence or non-existence of a disease, and a marker configured of specific secondary ion mass peaks.

Abstract translation: 本发明涉及疾病诊断方法，标记筛选方法和使用飞行时间二次离子质谱法（TOF-SIMS）的标记物，更具体地，涉及大肠癌诊断方法，大肠 癌症标志物筛选方法和使用飞行时间二次离子质谱（TOF-SIMS）的大肠癌标记物。 具体地，本发明提供使用飞行时间二次离子质谱法（TOF-SIMS）作为标记测定的生物样品的二次离子质量（m / z）峰图案，标记物 筛选方法是判断疾病的存在或不存在的参考，以及由特定二次离子质量峰构成的标记。