公开(公告)号：US06596506B2

公开(公告)日：2003-07-22

申请号：US09999504

申请日：2001-10-25

申请人： Tae Kook Kim

发明人： Tae Kook Kim

IPC分类号： C12Q102

CPC分类号： G01N33/5011 ,  C12Q1/6897

摘要： The present invention provides assays for identifying compounds that affect the transcriptional activity of a protein of interest or affect the stability of the protein of interest. The triple readout assay system which can be used to identify compounds that affect the transcriptional activity of a protein of interest uses three cell lines to control for non-specific effects such as sequences flanking the inserted gene and cytotoxicity. The double readout assay system assesses protein stability and uses a fusion protein of a reporter and the protein of interest. These assay systems may be particularly useful in identifying compounds that affect transcription factors and tumor suppressors. In a particular embodiment, the tumor suppressor p53 is the target protein being studied.

公开(公告)号：US09164090B2

公开(公告)日：2015-10-20

申请号：US12671881

申请日：2008-08-14

申请人： Tae Kook Kim ,  Seung Goo Lee ,  Sang Kyu Lee ,  Kyoung Hu Lee ,  Jae-Seok Ha

发明人： Tae Kook Kim ,  Seung Goo Lee ,  Sang Kyu Lee ,  Kyoung Hu Lee ,  Jae-Seok Ha

IPC分类号： C12Q1/68 ,  A61K38/00 ,  G01N33/543 ,  G01N33/50 ,  G01N33/536 ,  G01N33/68

CPC分类号： G01N33/54326 ,  G01N33/5008 ,  G01N33/536 ,  G01N33/54346 ,  G01N33/6845

摘要： The present invention relates to methods for dynamically detecting the interactions between various materials including bioactive molecules and for detecting target molecules. More specifically, the present invention relates to a method for dynamically detecting bait-prey interactions and a method for easily detecting target molecules which blocks or activates the interactions, the method comprising: allowing a material capable of forming a nano-assembly matrix, a bait and a prey to interact with each other, and analyzing whether a nano-assembly matrix is formed by the interaction between the bait and the prey in vitro or in vivo; or allowing a material capable of forming a nano-assembly matrix, a bait and a prey to interact with each other, inducing the formation of a nano-assembly matrix by a mediator (regulator) material in vitro or in vivo, and then analyzing whether the prey and the bait co-localizes on the nano-assembly matrix.

摘要翻译： 本发明涉及用于动态检测包括生物活性分子在内的各种材料与检测靶分子之间的相互作用的方法。 更具体地说，本发明涉及一种用于动态检测饵料捕食相互作用的方法和一种容易地检测阻碍或激活相互作用的靶分子的方法，所述方法包括：允许能够形成纳米组装体的材料，诱饵 和猎物相互作用，分析纳米组装体是否通过体外或体内诱饵和猎物之间的相互作用形成; 或允许能够形成纳米组装基质，诱饵和猎物彼此相互作用的材料，在体外或体内通过介体（调节剂）材料诱导形成纳米组装体，然后分析是否 猎物和诱饵共同定位在纳米装配矩阵上。

公开(公告)号：US20100273673A1

公开(公告)日：2010-10-28

申请号：US12671881

申请日：2008-08-14

申请人： Tae Kook Kim ,  Seung Goo Lee ,  Sang Kyu Lee ,  Kyoung Hu Lee ,  Jae-Seok Ha

发明人： Tae Kook Kim ,  Seung Goo Lee ,  Sang Kyu Lee ,  Kyoung Hu Lee ,  Jae-Seok Ha

IPC分类号： G01N33/53 ,  C40B30/04

CPC分类号： G01N33/54326 ,  G01N33/5008 ,  G01N33/536 ,  G01N33/54346 ,  G01N33/6845

摘要： The present invention relates to methods for dynamically detecting the interactions between various materials including bioactive molecules and for detecting target molecules. More specifically, the present invention relates to a method for dynamically detecting bait-prey interactions and a method for easily detecting target molecules which blocks or activates the interactions, the method comprising: allowing a material capable of forming a nano-assembly matrix, a bait and a prey to interact with each other, and analyzing whether a nano-assembly matrix is formed by the interaction between the bait and the prey in vitro or in vivo; or allowing a material capable of forming a nano-assembly matrix, a bait and a prey to interact with each other, inducing the formation of a nano-assembly matrix by a mediator (regulator) material in vitro or in vivo, and then analyzing whether the prey and the bait co-localizes on the nano-assembly matrix.

摘要翻译： 本发明涉及用于动态检测包括生物活性分子在内的各种材料与检测靶分子之间的相互作用的方法。 更具体地说，本发明涉及一种用于动态检测饵料捕食相互作用的方法和一种容易地检测阻碍或激活相互作用的靶分子的方法，所述方法包括：允许能够形成纳米组装体的材料，诱饵 和猎物相互作用，分析纳米组装体是否通过体外或体内诱饵和猎物之间的相互作用形成; 或允许能够形成纳米组装基质，诱饵和猎物彼此相互作用的材料，在体外或体内通过介体（调节剂）材料诱导形成纳米组装体，然后分析是否 猎物和诱饵共同定位在纳米装配矩阵上。

公开(公告)号：US09759717B2

公开(公告)日：2017-09-12

申请号：US14237840

申请日：2012-08-10

申请人： Tae Kook Kim

发明人： Tae Kook Kim

IPC分类号： G01N33/543 ,  C40B30/04 ,  B82Y15/00 ,  G01N33/542

CPC分类号： G01N33/54306 ,  B82Y15/00 ,  C40B30/04 ,  G01N33/542 ,  G01N33/54366

摘要： The present invention relates to methods of quantitatively analyzing and detecting interactions, which effectively occur between detector materials displayed on a nano-assembly matrix at high density, and sensitively labeling and isolating a target material that effectively interacts with the detector materials, by the use of energy transfer and signal changes that effectively occur in label molecules displayed at high density. Specifically, the present invention relates to a method of quantitatively detecting the interactions between various bioactive materials, which occur in vitro or in vivo, based on high-density displays of molecules, and to a method of sensitively labeling a target material that interacts with these bioactive materials.

公开(公告)号：US20140255314A1

公开(公告)日：2014-09-11

申请号：US14237832

申请日：2012-08-10

申请人： Tae Kook Kim

发明人： Tae Kook Kim

IPC分类号： G01N33/58 ,  A61K47/48 ,  G01N33/50 ,  A61B5/00 ,  A61N1/32

CPC分类号： G01N33/5023 ,  A61B5/0071 ,  A61K47/64 ,  A61N1/327 ,  B82Y15/00 ,  G01N33/54346

摘要： The present invention relates to a method of regulating or inducing specific physiological conditions or functions using one or more materials displayed on a nano-assembly matrix at high density in cells or in vivo. Specifically, the present invention relates to a method of effectively inducing specific physiological regulation in cells or in vivo by the high-density display of bioactive materials. According to the method of the invention, physiological regulation in cells or in vivo can be optionally induced by regulating the assembly and disassembly of nano assembly (unit) matrix or the display or trapping of specific materials on nano assembly (unit) matrix.

摘要翻译： 本发明涉及使用在细胞或体内以高密度显示在纳米组装体上的一种或多种材料调节或诱导特定生理条件或功能的方法。 具体而言，本发明涉及通过生物活性物质的高密度显示来有效诱导细胞或体内特异性生理调节的方法。 根据本发明的方法，可以通过调节纳米组件（单位）矩阵的组装和拆卸或者在纳米组件（单位）矩阵上显示或捕获特定材料来任意诱导细胞或体内的生理调节。

公开(公告)号：US20080242557A1

公开(公告)日：2008-10-02

申请号：US11572224

申请日：2005-07-20

申请人： Tae Kook Kim ,  Yong-Weon Yi ,  Jaejoon Won

发明人： Tae Kook Kim ,  Yong-Weon Yi ,  Jaejoon Won

IPC分类号： C40B30/06 ,  G01N21/76 ,  G01N23/00 ,  G01N25/22 ,  B01J19/00 ,  C40B30/10 ,  G01N33/543 ,  C12Q1/02

CPC分类号： G01N33/54366 ,  G01N33/54326

摘要： This invention relates to a method for detecting interactions between molecules, such as, biomolecules, and relates to a method for screening target molecules, both in vivo and in vitro. In this invention, the first detecting material and the second detecting material are provided, wherein the first detecting material is bound to a localizer which is translocated by externally applied driving force, and the second detecting material is bound to a label. Thus, the complex of the first detecting material and the second detecting material can be detected reversibly by changing the strength of the externally applied driving force, and the target molecule can be screened effectively.

摘要翻译： 本发明涉及用于检测分子（例如生物分子）之间的相互作用的方法，并且涉及在体内和体外筛选靶分子的方法。 在本发明中，提供了第一检测材料和第二检测材料，其中第一检测材料结合到通过外部施加的驱动力移位的定位器，并且第二检测材料结合到标签上。 因此，可以通过改变外部施加的驱动力的强度来可逆地检测第一检测材料和第二检测材料的复合物，并且可以有效地筛选目标分子。

公开(公告)号：US20140309132A1

公开(公告)日：2014-10-16

申请号：US14237840

申请日：2012-08-10

申请人： Tae Kook Kim

发明人： Tae Kook Kim

IPC分类号： G01N33/543

CPC分类号： G01N33/54306 ,  B82Y15/00 ,  C40B30/04 ,  G01N33/542 ,  G01N33/54366

摘要： The present invention relates to methods of quantitatively analyzing and detecting interactions, which effectively occur between detector materials displayed on a nano-assembly matrix at high density, and sensitively labeling and isolating a target material that effectively interacts with the detector materials, by the use of energy transfer and signal changes that effectively occur in label molecules displayed at high density. Specifically, the present invention relates to a method of quantitatively detecting the interactions between various bioactive materials, which occur in vitro or in vivo, based on high-density displays of molecules, and to a method of sensitively labeling a target material that interacts with these bioactive materials.

摘要翻译： 本发明涉及定量分析和检测相互作用的方法，这些相互作用有效发生在高密度纳米组装体基体上显示的检测器材料之间，并通过使用以下方式灵敏地标记和分离与检测器材料有效相互作用的目标材料 能量转移和信号变化在高密度显示的标签分子中有效发生。 具体而言，本发明涉及一种定量检测基于分子的高密度显示的体外或体内发生的各种生物活性物质之间的相互作用的方法，以及敏感地标记与这些相互作用的靶材料的方法 生物活性物质。