公开(公告)号：US20080242557A1

公开(公告)日：2008-10-02

申请号：US11572224

申请日：2005-07-20

申请人： Tae Kook Kim ,  Yong-Weon Yi ,  Jaejoon Won

发明人： Tae Kook Kim ,  Yong-Weon Yi ,  Jaejoon Won

IPC分类号： C40B30/06 ,  G01N21/76 ,  G01N23/00 ,  G01N25/22 ,  B01J19/00 ,  C40B30/10 ,  G01N33/543 ,  C12Q1/02

CPC分类号： G01N33/54366 ,  G01N33/54326

摘要： This invention relates to a method for detecting interactions between molecules, such as, biomolecules, and relates to a method for screening target molecules, both in vivo and in vitro. In this invention, the first detecting material and the second detecting material are provided, wherein the first detecting material is bound to a localizer which is translocated by externally applied driving force, and the second detecting material is bound to a label. Thus, the complex of the first detecting material and the second detecting material can be detected reversibly by changing the strength of the externally applied driving force, and the target molecule can be screened effectively.

摘要翻译： 本发明涉及用于检测分子（例如生物分子）之间的相互作用的方法，并且涉及在体内和体外筛选靶分子的方法。 在本发明中，提供了第一检测材料和第二检测材料，其中第一检测材料结合到通过外部施加的驱动力移位的定位器，并且第二检测材料结合到标签上。 因此，可以通过改变外部施加的驱动力的强度来可逆地检测第一检测材料和第二检测材料的复合物，并且可以有效地筛选目标分子。

公开(公告)号：US06204242B1

公开(公告)日：2001-03-20

申请号：US09381559

申请日：2000-01-19

申请人： Insoo Bae ,  Dong-soo Kim ,  Heajoon Yim ,  Neon-Cheol Jung ,  Yong-Weon Yi ,  Seung-Suh Hong ,  Hyun-Soo Lee

发明人： Insoo Bae ,  Dong-soo Kim ,  Heajoon Yim ,  Neon-Cheol Jung ,  Yong-Weon Yi ,  Seung-Suh Hong ,  Hyun-Soo Lee

IPC分类号： A61K3800

CPC分类号： A61K38/1709

摘要： The present invention relates to a novel use of histone and provides a pharmaceutical composition containing histone as an active ingredient to improve the symptoms of progressive, inflammatory and autoimmune arthritis. The pharmaceutical composition of the present invention includes histone, especially histone H1 as an active ingredient, and could include pharmacologically approved carriers if necessary. Histone H1 lowered induction of arthritis and reduced arthritis index more effectively than steroidal dexamethasone and also had a significant preventive effect.

摘要翻译： 本发明涉及组蛋白的新用途，并提供含有组蛋白作为活性成分以改善进行性，炎性和自身免疫性关节炎症状的药物组合物。 本发明的药物组合物包括组蛋白，特别是组蛋白H1作为活性成分，如果需要，可以包括药理学上认可的载体。 组蛋白H1比甾体地塞米松更有效地降低了关节炎的诱导，降低了关节炎指数，并且具有显着的预防作用。

公开(公告)号：US07319001B2

公开(公告)日：2008-01-15

申请号：US10383838

申请日：2003-03-07

申请人： Ho-Sun Son ,  Do-Hui Kim ,  Neon-C Jung ,  Eun-Wook Choi ,  Dong-Seung Seen ,  Min-Sung Kim ,  Yong-Weon Yi ,  Kyung-Jin Kim

发明人： Ho-Sun Son ,  Do-Hui Kim ,  Neon-C Jung ,  Eun-Wook Choi ,  Dong-Seung Seen ,  Min-Sung Kim ,  Yong-Weon Yi ,  Kyung-Jin Kim

IPC分类号： C12N15/10 ,  C12N15/63 ,  C12N15/64 ,  C12N15/866 ,  C12N15/86 ,  A61K48/00

CPC分类号： C12N15/86 ,  C12N15/1027 ,  C12N2710/14143

摘要： Disclosed are the methods for producing recombinant viruses using site-specific recombination in vitro. In the present invention, circular viral genomic DNAs are digested with restriction enzymes to generate a linear form viral genomic DNAs flanked by site-specific recombination sites, and then are subjected to site-specific recombination with the desired genomic materials flanked by site-specific recombination sites in vitro. According to the present invention, since the site-specific recombination mixture can be applied to host cells without further procedures of selecting the desired recombinant viral genomic DNAs, it is possible to obtain numerous recombinant viruses rapidly at the same time. Thus, the present invention can be used as a high throughput system for generating and screening hundreds or thousands of recombinant viruses.

摘要翻译： 公开了使用体外位点特异性重组生产重组病毒的方法。 在本发明中，循环病毒基因组DNA用限制性酶消化以产生侧翼为位点特异性重组位点的线性形式的病毒基因组DNA，然后进行与所需基因组物质的位点特异性重组，所述基因组物质侧接于位点特异性重组 体外试验。 根据本发明，由于可以不用进一步选择所需的重组病毒基因组DNA的方式将位点特异性重组混合物施用于宿主细胞，所以可以同时快速获得多种重组病毒。 因此，本发明可以用作产生和筛选数百或数千种重组病毒的高通量系统。