公开(公告)号：US10196603B2

公开(公告)日：2019-02-05

申请号：US15668256

申请日：2017-08-03

申请人： The United States of America as represented by the Administrator or NASA

发明人： Lisa A. Scott Carnell ,  Emilie J. Siochi ,  Nancy M. Holloway ,  Kam W. Leong ,  Karina Kulangara

IPC分类号： C12N5/00 ,  C12N11/08 ,  A61L27/38 ,  A61L27/16 ,  A61K35/28 ,  C12N13/00 ,  A61K35/12

摘要： A method of manufacturing and/or using a scaffold assembly for stem cell culture and tissue engineering applications is disclosed. The scaffold at least partially mimics a native biological environment by providing biochemical, topographical, mechanical and electrical cues by using an electroactive material. The assembly includes at least one layer of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application. A method of cell tissue engineering and/or stem cell differentiation that uses the assembly seeded with a sample of cells suspended in cell culture media, incubates and applies voltage to one or more layers, and thus produces cells and/or a tissue construct. In another aspect, the invention provides a method of manufacturing the assembly including the steps of providing a first pre-electroded substrate surface; electrospinning a first substantially aligned polymer fiber layer onto the first surface; providing a second pre-electroded substrate surface; electrospinning a second substantially aligned polymer fiber layer onto the second surface; and, retaining together the layered surfaces with a clamp and/or an adhesive compound.