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1.发明申请S-Adenosylmethionine Synthetase Mutants, the DNAs Encoding the Same and Uses of the Mutants 有权S-腺苷甲硫氨酸合成酶突变体，编码它们的DNA和突变体的用途公开(公告)号：US20090203080A1
公开(公告)日：2009-08-13
申请号：US12438680
申请日：2007-08-30
申请人： Rongzhao Fu , Tianzuo Zhang , Jun Wang
发明人： Rongzhao Fu , Tianzuo Zhang , Jun Wang
CPC分类号： C12N9/1085 , C12Y205/01006
摘要： The invention discloses a series of Methanococcus jannaschii S-adenosylmethionine synthetase mutants with improved thermostability and high catalytic activity obtained by using gene mutation technique, characterized in that these mutants refer to an enzyme using Sequence 2 in the Sequence Listing as the reference sequence and contains at least one mutation at position 102, position 93, position 230, and position 357 and has a catalytic activity at least 70% higher than that of the wild-type S-adenosylmethionine synthetase using adenosine triphosphate (ATP) and methionine as substrates. These S-adenosylmethionine synthetase mutants can be used in the production of S-adenosylmethionine.
摘要翻译： 本发明公开了一种通过使用基因突变技术获得的具有改善的热稳定性和高催化活性的甲烷球菌S-腺苷甲硫氨酸合成酶突变体，其特征在于，这些突变体是指使用序列表中的序列2作为参考序列的酶， 位置102，位置93，位置230和位置357处的至少一个突变，并且具有比使用三磷酸腺苷（ATP）和甲硫氨酸作为底物的野生型S-腺苷甲硫氨酸合成酶高至少70％的催化活性。 这些S-腺苷甲硫氨酸合成酶突变体可用于S-腺苷甲硫氨酸的生产。
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2.发明授权S-adenosylmethionine synthetase mutants, the DNAs encoding the same and uses of the mutants 有权S-腺苷甲硫氨酸合成酶突变体，编码相同的DNA和突变体的用途公开(公告)号：US07858351B2
公开(公告)日：2010-12-28
申请号：US12438680
申请日：2007-08-30
申请人： Rongzhao Fu , Tianzuo Zhang , Jun Wang
发明人： Rongzhao Fu , Tianzuo Zhang , Jun Wang
CPC分类号： C12N9/1085 , C12Y205/01006
摘要： The invention discloses a series of Methanococcus jannaschii S-adenosylmethionine synthetase mutants with improved thermostability and high catalytic activity obtained by using gene mutation technique, characterized in that these mutants refer to an enzyme using Sequence 2 in the Sequence Listing as the reference sequence and contains at least one mutation at position 102, position 93, position 230, and position 357 and has a catalytic activity at least 70% higher than that of the wild-type S-adenosylmethionine synthetase using adenosine triphosphate (ATP) and methionine as substrates. These S-adenosylmethionine synthetase mutants can be used in the production of S-adenosylmethionine.
摘要翻译： 本发明公开了一种通过使用基因突变技术获得的具有改善的热稳定性和高催化活性的甲烷球菌S-腺苷甲硫氨酸合成酶突变体，其特征在于，这些突变体是指使用序列表中的序列2作为参考序列的酶， 位置102，位置93，位置230和位置357处的至少一个突变，并且具有比使用三磷酸腺苷（ATP）和甲硫氨酸作为底物的野生型S-腺苷甲硫氨酸合成酶高至少70％的催化活性。 这些S-腺苷甲硫氨酸合成酶突变体可用于S-腺苷甲硫氨酸的生产。
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