公开(公告)号：US4743554A

公开(公告)日：1988-05-10

申请号：US728464

申请日：1985-05-01

申请人： John C. Boothroyd ,  George A. M. Cross ,  Michael D. Highfield ,  Michael D. Winther ,  David J. Rowlands ,  Fred Brown ,  Timothy J. R. Harris

发明人： John C. Boothroyd ,  George A. M. Cross ,  Michael D. Highfield ,  Michael D. Winther ,  David J. Rowlands ,  Fred Brown ,  Timothy J. R. Harris

IPC分类号： A61K39/00 ,  C07K14/09 ,  C12N1/00 ,  C12N1/20 ,  C12N15/00

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2770/32122 ,  Y10S930/222

摘要： A DNA molecule comprising a nucleotide sequence substantially corresponding to all or a portion of foot and mouth disease virus RNA and in particular coding for at least one protein of foot and mouth disease virus. The DNA molecule can be inserted into a DNA cloning vehicle capable of expressing the DNA molecule, after a suitable host cell has been transformed by the cloning vehicle. The expression product can be incorporated into a vaccine for stimulating antibodies against FMDV. Methods for producing the DNA molecule, recombinant cloning vehicle and transformed host cell are described.

摘要翻译： DNA分子，其包含基本上对应于口蹄疫病毒RNA的全部或部分的核苷酸序列，特别是编码至少一种口蹄疫病毒蛋白质。 在通过克隆载体转化合适的宿主细胞后，可以将DNA分子插入能够表达DNA分子的DNA克隆载体中。 表达产物可以并入用于刺激针对FMDV的抗体的疫苗中。 描述了生产DNA分子，重组克隆载体和转化的宿主细胞的方法。

公开(公告)号：US5332805A

公开(公告)日：1994-07-26

申请号：US677415

申请日：1991-03-29

申请人： Norman H. Carey ,  Michael T. Doel ,  Timothy J. R. Harris ,  Peter A. Lowe

发明人： Norman H. Carey ,  Michael T. Doel ,  Timothy J. R. Harris ,  Peter A. Lowe

IPC分类号： C12N9/64 ,  C07K3/12 ,  C07K15/06

CPC分类号： C12N9/6481

摘要： The production of recombinant chymosin is disclosed in which an insoluble form of chymosin precursor is produced by a bacterial host cell transformed by a vector including a coding sequence for said precursor. Solubilization of said insoluble form of chymosin precursor is accomplished using urea at a concentration of at least 7M or guanidine hydrochloride at a concentration of at least 6M prior to cleaving said precursor to form chymosin. Said solubilization preferably additionally involves the denaturation of said precursor in an alkaline aqueous solution, e.g., at a pH between a 9 and 11.5

摘要翻译： 公开了重组凝乳酶的生产，其中由包含所述前体的编码序列的载体转化的细菌宿主细胞产生不溶性形式的凝乳酶前体。 所述不溶形式的凝乳酶前体的溶解是在切割所述前体以形成凝乳酶之前，使用至少7M浓度的尿素或至少6M浓度的盐酸胍。 所述溶解优选另外包括在碱性水溶液中，例如在9和11之间的pH下变性所述前体。