公开(公告)号：US5344777A

公开(公告)日：1994-09-06

申请号：US985458

申请日：1992-12-03

Applicant: Toshimi Tamaki ,  Hiroshi Takemura ,  Kenji Tayama ,  Masahiro Fukaya ,  Hajime Okumura ,  Yoshiya Kawamura

Inventor： Toshimi Tamaki ,  Hiroshi Takemura ,  Kenji Tayama ,  Masahiro Fukaya ,  Hajime Okumura ,  Yoshiya Kawamura

IPC: C12N9/04 ,  C07K16/40 ,  C12N1/21 ,  C12N15/09 ,  C12N15/53 ,  C12R1/01 ,  C12R1/02 ,  C12N15/31 ,  C12N15/74

CPC classification number: C07K16/40 ,  C12N9/0006 ,  Y10S435/823

Abstract: There is provided a structural gene of membrane-bound alcohol dehydrogenase complex having a molecular size of about 7.0 kilo base which is derived from a microorganism belonging to the genus Acetobacter represented by Acetobacter altoacetigenes and shown by the nucleotide sequence of SEQ ID. NO. 1 and SEQ ID NO. 2. This enzyme increases the efficiency of acetic acid fermentation and may be effectively utilized for quantitative determination of alcohol.

Abstract translation: 本发明提供一种具有约7.0千碱基的膜结合醇脱氢酶复合物的结构基因，该结构基因来源于由醋杆菌属（Acetobacter altoacetigenes）代表的属于醋杆菌属的微生物，由SEQ ID NO： 没有。 1和SEQ ID NO： 这种酶提高了乙酸发酵的效率，可以有效地用于定量测定酒精。

公开(公告)号：US5914257A

公开(公告)日：1999-06-22

申请号：US987260

申请日：1992-12-07

Applicant: Masahiro Fukaya ,  Kenji Tayama ,  Toshimi Tamaki ,  Haruko Izumo ,  Hajime Okumura ,  Yoshiya Kawamura

Inventor： Masahiro Fukaya ,  Kenji Tayama ,  Toshimi Tamaki ,  Haruko Izumo ,  Hajime Okumura ,  Yoshiya Kawamura

IPC: C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N15/09 ,  C12N15/53 ,  C12P7/54 ,  C12R1/02

CPC classification number: C12N9/0008 ,  C12P7/54 ,  C12Y102/01003 ,  C12Y102/01004 ,  C12Y102/01005

Abstract: This invention relates to the structural gene for the membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter, said structural gene having a molecular size of about 3.6 Kb and having a restriction enzyme cleavage map as shown in FIG. 1; to a plasmid containing said structural gene; to an acetic acid bacterium transformed by said plasmid; and to an acetic acid fermentation process using said transformant.

Abstract translation: 本发明涉及来自属于醋杆菌属的微生物的膜结合醛脱氢酶的结构基因，所述结构基因具有约3.6Kb的分子大小，并具有如图1所示的限制酶切割图。 1; 涉及含有所述结构基因的质粒; 向由所述质粒转化的乙酸细菌; 以及使用所述转化体的乙酸发酵方法。