公开(公告)号：US5122603A

公开(公告)日：1992-06-16

申请号：US320484

申请日：1989-03-08

申请人： Joseph Larner ,  Alison Kennington ,  Laura Huang ,  Tsung Y. Shen

发明人： Joseph Larner ,  Alison Kennington ,  Laura Huang ,  Tsung Y. Shen

IPC分类号： G01N33/50 ,  A61K31/70 ,  A61K35/407 ,  A61K49/00 ,  A61P3/08 ,  A61P43/00 ,  C07K1/14 ,  C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K14/00 ,  C07K14/47 ,  G01N30/88 ,  C07K15/00 ,  A01N57/00 ,  C12N9/88

CPC分类号： C07K14/47 ,  Y10S514/866

摘要： A method for purifying two distinct insulin mediators to substantial homogeneity, and relative purity values above 80%, comprises adsorption on first anion exchange resin and subsequently on a chelex cation exchange resin column. The chelex resin ion exchange column substantially increases the activity of the recovered mediator. Following purification, the already treated fraction is subjected to three successive thin layer chromatography purification steps, the last giving, in the presence of ninhydrin stain, a characteristic salmon-color spot, which is indicative of the presence of the mediator. This process can also be used as a screening process, the absence of the salmon-colored spot being indicative of the diabetic state. Structure information on the insulin mediators obtained is given.

摘要翻译： 一种用于纯化两种不同胰岛素介质以达到基本均匀的方法，并且相对纯度值高于80％的方法包括在第一阴离子交换树脂上吸附，随后在Chelex阳离子交换树脂柱上进行吸附。 衣壳树脂离子交换柱显着增加了回收的介体的活性。 纯化后，已经处理的级分经过三次连续的薄层色谱纯化步骤，最后在茚三酮染色剂存在下给出特征性鲑鱼色斑，这表明存在介体。 该过程也可用作筛选过程，没有鲑鱼色斑点指示糖尿病状态。 给出了获得的胰岛素介质的结构信息。