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    Expression cassettes for the bi-directional transgenic expression of nucleic acids in plants
    4.
    发明授权
    Expression cassettes for the bi-directional transgenic expression of nucleic acids in plants 失效
    用于植物中核酸双向转录表达的表达盒

    公开(公告)号：US07557203B2

    公开(公告)日：2009-07-07

    申请号：US10565221

    申请日：2004-07-03

    申请人： Ute Linemann ,  Karin Herbers ,  Irene Kunze

    发明人： Ute Linemann ,  Karin Herbers ,  Irene Kunze

    IPC分类号： C12N15/82 ,  C12N15/00 ,  A01H5/00 ,  A01H5/10 ,  C12N15/11 ,  C12N15/63

    CPC分类号： C12N15/8216

    摘要： The invention relates to transgenic expression cassettes for expressing two nucleic acid sequences in a plant cell comprising at least one regulatory sequence selected from the group consisting of a) the promoter shown in SEQ ID NO: 1 or 2, b) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which have an identity of at least 80% to the sequence shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, c) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which comprise at least 25 consecutive nucleotides of the sequences shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, and d) functionally equivalent fragments of sequences a) or b) or c), which have at least 25 consecutive nucleotides of said sequences a) or b) or c) and have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, where said regulatory element is disposed between two nucleic acid sequences and is heterogeneous in relation to said nucleic acid sequences and is functionally linked to said nucleic acid sequences in such a way that the expression of two different ribonucleic acid sequences is brought about in at least one plant cell, where said ribonucleic acid sequences are selected from ribonucleic acid sequences coding for i) amino acid sequences or ii) ribonucleic acid sequences which bring about a reduction in the expression of at least one endogenous gene of said plant cell.

    摘要翻译： 本发明涉及用于在植物细胞中表达两种核酸序列的转基因表达盒，其包含至少一种选自a）SEQ ID NO：1或2所示的启动子的调节序列，b）启动子的功能等同物 如SEQ ID NO：1或2所示，其与SEQ ID NO：1或2所示的序列具有至少80％的同一性，并且与SEQ ID NO：1或2所示的启动子具有基本上相同的启动子活性 ，c）SEQ ID NO：1或2所示的启动子的功能等同物，其包含SEQ ID NO：1或2所示序列的至少25个连续核苷酸并且与SEQ ID NO：1或2所示的启动子具有基本上相同的启动子活性 SEQ ID NO：1或2，以及d）序列a）或b）或c）的功能上等同的片段，其具有所述序列a）或b）或c）的至少25个连续核苷酸，并且具有基本上相同的启动子活性 SEQ ID NO：1或2所示的启动子， 其中所述调节元件设置在两个核酸序列之间并且与所述核酸序列相关并且与所述核酸序列功能性连接，使得两个不同的核糖核酸序列的表达在至少一个 植物细胞，其中所述核糖核酸序列选自编码i）氨基酸序列的核糖核酸序列或ii）导致所述植物细胞的至少一个内源基因表达降低的核糖核酸序列。

    Expression cassettes for the bi-directional transgenic expression of nucleic acids in plants
    5.
    发明申请
    Expression cassettes for the bi-directional transgenic expression of nucleic acids in plants 失效

    公开(公告)号：US20060150282A1

    公开(公告)日：2006-07-06

    申请号：US10565221

    申请日：2004-07-03

    申请人： Ute Linemann ,  Karin Herbers ,  Irene Kunze

    发明人： Ute Linemann ,  Karin Herbers ,  Irene Kunze

    IPC分类号： C12N5/04 ,  C12N15/82 ,  A01H1/00

    CPC分类号： C12N15/8216

    摘要： The invention relates to transgenic expression cassettes for expressing two nucleic acid sequences in a plant cell comprising at least one regulatory sequence selected from the group consisting of a) the promoter shown in SEQ ID NO: 1 or 2, b) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which have an identity of at least 80% to the sequence shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, c) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which comprise at least 25 consecutive nucleotides of the sequences shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, and d) functionally equivalent fragments of sequences a) or b) or c), which have at least 25 consecutive nucleotides of said sequences a) or b) or c) and have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, where said regulatory element is disposed between two nucleic acid sequences and is heterogeneous in relation to said nucleic acid sequences and is functionally linked to said nucleic acid sequences in such a way that the expression of two different ribonucleic acid sequences is brought about in at least one plant cell, where said ribonucleic acid sequences are selected from ribonucleic acid sequences coding for i) amino acid sequences or ii) ribonucleic acid sequences which bring about a reduction in the expression of at least one endogenous gene of said plant cell.

    Constitutive expression cassettes for regulation of plant expression
    7.
    发明授权
    Constitutive expression cassettes for regulation of plant expression 失效
    用于调节植物表达的组成型表达盒

    公开(公告)号：US08519114B2

    公开(公告)日：2013-08-27

    申请号：US13282973

    申请日：2011-10-27

    申请人： Ulrich Keetman ,  Ute Linemann ,  Karin Herbers ,  Helke Hillebrand

    发明人： Ulrich Keetman ,  Ute Linemann ,  Karin Herbers ,  Helke Hillebrand

    IPC分类号： C12N15/82 ,  C12N15/87 ,  C12N15/63

    CPC分类号： C12N15/8216

    摘要： The present invention relates to expression cassettes comprising transcription regulating sequences with constitutive expression profiles in plants obtainable from Arabidopsis thaliana genes At5g17920, At3g03780, At2g01100, At2g34770, At5g61560, At4g00830, At3g10220, At4g38520, At3g11110, At2g47170, Atlg64090, At5g60690, Atlg76350, At1g76580, Atlg31930, At5g18230, Atlg20970, or At4g35620. The expression cassettes according to the present invention may be employed for expression of a protein, or expression of an antisense RNA, sense or double-stranded RNA. Preferably, expression of the nucleic acid sequence confers to the plant an agronomically valuable trait. Vectors comprising such an expression cassette, and transgenic host cell or non-human organism comprising the expression cassette or the vector are also enclosed. Also enclosed is a method for identifying and/or isolating a sequence with constitutive transcription regulating activity.

    摘要翻译： 本发明涉及含有从拟南芥基因At5g17920，At3g03780，At2g01100，At2g34770，At5g61560，At4g00830，At3g10220，At4g38520，At3g11110，At2g47170，Atlg64090，At5g60690，Atlg76350，At1g76580中获得的植物中具有组成型表达谱的转录调控序列的表达盒， Atlg31930，At5g18230，Atlg20970或At4g35620。 根据本发明的表达盒可用于表达蛋白质，或表达反义RNA，正义或双链RNA。 优选地，核酸序列的表达赋予植物农学上有价值的性状。 包含这种表达盒的载体和包含表达盒或载体的转基因宿主细胞或非人生物也被封闭。 还包括用于鉴定和/或分离具有组成型转录调节活性的序列的方法。

    Expression cassettes for root-preferential expression in plants
    8.
    发明授权
    Expression cassettes for root-preferential expression in plants 失效
    表达盒在植物中优先表达

    公开(公告)号：US07999150B2

    公开(公告)日：2011-08-16

    申请号：US12648651

    申请日：2009-12-29

    申请人： Ulrich Keetman ,  Ute Linemann ,  Karin Herbers ,  Helke Hillebrand

    发明人： Ulrich Keetman ,  Ute Linemann ,  Karin Herbers ,  Helke Hillebrand

    IPC分类号： C12N15/82 ,  C12N15/63 ,  A01H5/00

    CPC分类号： C12N15/8227 ,  C07K14/415

    摘要： The present invention relates to expression cassettes comprising transcription regulating sequences with root-preferential or root-specific expression profiles in plants obtainable from Arabidopsis thaliana genes At1g66280, At1g74500, At1g49570, At1g70710, At5g66690, At3g29630, At5g48070, or At4g17800. The expression cassettes according to the present invention may be employed for expression of a protein, or expression of an antisense RNA, sense or double-stranded RNA. Preferably, expression of the nucleic acid sequence confers to the plant an agronomically valuable trait. Vectors comprising such expression cassette, and transgenic host cell or non-human organism comprising the expression cassette or the vector is also enclosed. Also enclosed is a method for identifying and/or isolating a sequence with root-specific or root-preferential transcription regulating activity.

    摘要翻译： 本发明涉及包含从拟南芥基因At1g66280，At1g74500，At1g49570，At1g70710，At5g66690，At3g29630，At5g48070或At4g17800获得的植物中具有根优先或根特异性表达谱的转录调节序列的表达盒。 根据本发明的表达盒可用于表达蛋白质，或表达反义RNA，正义或双链RNA。 优选地，核酸序列的表达赋予植物农学上有价值的性状。 包含该表达盒的载体和包含表达盒或载体的转基因宿主细胞或非人生物体也被封闭。 还包括用于鉴定和/或分离具有根特异性或根优先转录调节活性的序列的方法。

    Expression cassettes for root-preferential expression in plants
    9.
    发明授权
    Expression cassettes for root-preferential expression in plants 失效
    表达盒在植物中优先表达

    公开(公告)号：US07667098B2

    公开(公告)日：2010-02-23

    申请号：US12041253

    申请日：2008-03-03

    申请人： Ulrich Keetman ,  Ute Linemann ,  Karin Herbers ,  Helke Hillebrand

    发明人： Ulrich Keetman ,  Ute Linemann ,  Karin Herbers ,  Helke Hillebrand

    IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N15/87 ,  C07H21/04 ,  C12N15/00

    CPC分类号： C12N15/8227 ,  C07K14/415

    摘要： The present invention relates to expression cassettes comprising transcription regulating sequences with root-preferential or root-specific expression profiles in plants obtainable from Arabidopsis thaliana genes At1g66280, At1g74500, At1g49570, At1g70710, At5g66690, At3g29630, At5g48070, or At4g17800. The expression cassettes according to the present invention may be employed for expression of a protein, or expression of an antisense RNA, sense or double-stranded RNA. Preferably, expression of the nucleic acid sequence confers to the plant an agronomically valuable trait. Vectors comprising such expression cassette, and transgenic host cell or non-human organism comprising the expression cassette or the vector is also enclosed. Also enclosed is a method for identifying and/or isolating a sequence with root-specific or root-preferential transcription regulating activity.

    摘要翻译： 本发明涉及包含从拟南芥基因At1g66280，At1g74500，At1g49570，At1g70710，At5g66690，At3g29630，At5g48070或At4g17800获得的植物中具有根优先或根特异性表达谱的转录调节序列的表达盒。 根据本发明的表达盒可用于表达蛋白质，或表达反义RNA，正义或双链RNA。 优选地，核酸序列的表达赋予植物农学上有价值的性状。 包含该表达盒的载体和包含表达盒或载体的转基因宿主细胞或非人生物体也被封闭。 还包括用于鉴定和/或分离具有根特异性或根优先转录调节活性的序列的方法。