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    Expression cassettes for the bi-directional transgenic expression of nucleic acids in plants
    6.
    发明授权
    Expression cassettes for the bi-directional transgenic expression of nucleic acids in plants 失效
    用于植物中核酸双向转录表达的表达盒

    公开(公告)号：US07557203B2

    公开(公告)日：2009-07-07

    申请号：US10565221

    申请日：2004-07-03

    申请人： Ute Linemann ,  Karin Herbers ,  Irene Kunze

    发明人： Ute Linemann ,  Karin Herbers ,  Irene Kunze

    IPC分类号： C12N15/82 ,  C12N15/00 ,  A01H5/00 ,  A01H5/10 ,  C12N15/11 ,  C12N15/63

    CPC分类号： C12N15/8216

    摘要： The invention relates to transgenic expression cassettes for expressing two nucleic acid sequences in a plant cell comprising at least one regulatory sequence selected from the group consisting of a) the promoter shown in SEQ ID NO: 1 or 2, b) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which have an identity of at least 80% to the sequence shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, c) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which comprise at least 25 consecutive nucleotides of the sequences shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, and d) functionally equivalent fragments of sequences a) or b) or c), which have at least 25 consecutive nucleotides of said sequences a) or b) or c) and have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, where said regulatory element is disposed between two nucleic acid sequences and is heterogeneous in relation to said nucleic acid sequences and is functionally linked to said nucleic acid sequences in such a way that the expression of two different ribonucleic acid sequences is brought about in at least one plant cell, where said ribonucleic acid sequences are selected from ribonucleic acid sequences coding for i) amino acid sequences or ii) ribonucleic acid sequences which bring about a reduction in the expression of at least one endogenous gene of said plant cell.

    摘要翻译： 本发明涉及用于在植物细胞中表达两种核酸序列的转基因表达盒，其包含至少一种选自a）SEQ ID NO：1或2所示的启动子的调节序列，b）启动子的功能等同物 如SEQ ID NO：1或2所示，其与SEQ ID NO：1或2所示的序列具有至少80％的同一性，并且与SEQ ID NO：1或2所示的启动子具有基本上相同的启动子活性 ，c）SEQ ID NO：1或2所示的启动子的功能等同物，其包含SEQ ID NO：1或2所示序列的至少25个连续核苷酸并且与SEQ ID NO：1或2所示的启动子具有基本上相同的启动子活性 SEQ ID NO：1或2，以及d）序列a）或b）或c）的功能上等同的片段，其具有所述序列a）或b）或c）的至少25个连续核苷酸，并且具有基本上相同的启动子活性 SEQ ID NO：1或2所示的启动子， 其中所述调节元件设置在两个核酸序列之间并且与所述核酸序列相关并且与所述核酸序列功能性连接，使得两个不同的核糖核酸序列的表达在至少一个 植物细胞，其中所述核糖核酸序列选自编码i）氨基酸序列的核糖核酸序列或ii）导致所述植物细胞的至少一个内源基因表达降低的核糖核酸序列。

    Expression cassettes for the bi-directional transgenic expression of nucleic acids in plants
    8.
    发明申请
    Expression cassettes for the bi-directional transgenic expression of nucleic acids in plants 失效

    公开(公告)号：US20060150282A1

    公开(公告)日：2006-07-06

    申请号：US10565221

    申请日：2004-07-03

    申请人： Ute Linemann ,  Karin Herbers ,  Irene Kunze

    发明人： Ute Linemann ,  Karin Herbers ,  Irene Kunze

    IPC分类号： C12N5/04 ,  C12N15/82 ,  A01H1/00

    CPC分类号： C12N15/8216

    摘要： The invention relates to transgenic expression cassettes for expressing two nucleic acid sequences in a plant cell comprising at least one regulatory sequence selected from the group consisting of a) the promoter shown in SEQ ID NO: 1 or 2, b) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which have an identity of at least 80% to the sequence shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, c) functional equivalents of the promoter shown in SEQ ID NO: 1 or 2 which comprise at least 25 consecutive nucleotides of the sequences shown in SEQ ID NO: 1 or 2 and which have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, and d) functionally equivalent fragments of sequences a) or b) or c), which have at least 25 consecutive nucleotides of said sequences a) or b) or c) and have substantially the same promoter activity as the promoter shown in SEQ ID NO: 1 or 2, where said regulatory element is disposed between two nucleic acid sequences and is heterogeneous in relation to said nucleic acid sequences and is functionally linked to said nucleic acid sequences in such a way that the expression of two different ribonucleic acid sequences is brought about in at least one plant cell, where said ribonucleic acid sequences are selected from ribonucleic acid sequences coding for i) amino acid sequences or ii) ribonucleic acid sequences which bring about a reduction in the expression of at least one endogenous gene of said plant cell.