公开(公告)号：US20050069933A1

公开(公告)日：2005-03-31

申请号：US10940964

申请日：2004-09-15

申请人： Valerie Cheynet-Sauvion ,  Nadege Arnaud-Barbe ,  Guy Oriol ,  William McAllister ,  Bernard Mandrand ,  Francois Mallet

发明人： Valerie Cheynet-Sauvion ,  Nadege Arnaud-Barbe ,  Guy Oriol ,  William McAllister ,  Bernard Mandrand ,  Francois Mallet

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/12 ,  C12N15/54 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/22 ,  C12N15/74 ,  C12Q1/70

CPC分类号： C12Q1/6865 ,  C12N9/127 ,  C12Q2521/119

摘要： RNA may be transcribed using a nucleotide reagent as the promoter. The reagent may enable RNA to be transcribed without sequence specification and without protein cofactors, by means of an RNA polymerase that is known to be DNA-dependent such as the RNA polymerase of the phage T7, or by means of new, mutated RNA polymerase with the ability to synthesize a transcription product of polynucleotide matrix with a higher yield when the matrix is RNA than when the matrix is DNA. This type of RNA polymerase can be obtained by effecting mutations on a coding gene for a wild-type RNA polymerase, and then by selecting the mutated RNA polymerase with the ability. The invention can be applied notably to the detection, synthesis or quantification of RNA.

摘要翻译： 可以使用核苷酸试剂作为启动子来转录RNA。 通过已知DNA依赖性的RNA聚合酶，例如噬菌体T7的RNA聚合酶，或者通过新的突变的RNA聚合酶，使试剂可以使RNA不经序列指定而没有蛋白质辅因子转录， 当基质是RNA时，与基质是DNA相比，合成具有更高收率的多核苷酸基质的转录产物的能力。 可以通过对野生型RNA聚合酶的编码基因进行突变，然后通过选择具有该能力的突变的RNA聚合酶来获得这种类型的RNA聚合酶。 本发明可以应用于RNA的检测，合成或定量。