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1.发明申请公开(公告)号：US20060063240A1
公开(公告)日：2006-03-23
申请号：US11222983
申请日：2005-09-12
申请人： Joanna Katashkina , Maria Lunts , Vera Doroshenko , Svetlana Fomina , Aleksandra Skorokhodova , Lirina Ivanovskaya , Sergei Mashko
发明人： Joanna Katashkina , Maria Lunts , Vera Doroshenko , Svetlana Fomina , Aleksandra Skorokhodova , Lirina Ivanovskaya , Sergei Mashko
摘要： A method is provided for obtaining an L-amino acid or nucleic acid-producing bacterium belonging to the genus Escherichia with an optimized level of expression of the gene which influences the distribution of carbon flow, such as the sucAB genes, comprising introducing into the chromosome of the bacterium a set of in vitro constructed DNA fragments which contain regulatory elements for gene expression instead of the native elements of the regulatory region of the gene, and selecting the colonies with increased L-amino acid productivity. Also, a method is provided for producing an L-amino acid, such as L-glutamic acid, L-proline, L-arginine, L-glutamine, L-leucine, using the bacterium with an optimized level of expression of the sucAB gene.
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公开(公告)号：US20050191684A1
公开(公告)日：2005-09-01
申请号：US11063223
申请日：2005-02-23
申请人： Danila Zimenkov , Andrey Gulevich , Aleksandra Skorokhodova , Joanna Katashkina , Aleksandr Kivero , Irina Biryukova , Vera Doroshenko , Sergei Mashko
发明人： Danila Zimenkov , Andrey Gulevich , Aleksandra Skorokhodova , Joanna Katashkina , Aleksandr Kivero , Irina Biryukova , Vera Doroshenko , Sergei Mashko
摘要： A method for producing L-amino acids, such as L-tryptophan, L-phenylalanine, and L-tyrosine, using a bacterium of the Enterobacteriaceae family is provided. The L-amino acid productivity of said bacterium is increased by enhancing an activity of 6-phosphogluconolactonase, which is encoded by the pgl gene (ybhE ORF).
摘要翻译： 提供了使用肠杆菌科的细菌生产L-氨基酸如L-色氨酸，L-苯丙氨酸和L-酪氨酸的方法。 所述细菌的L-氨基酸生产率通过提高由pgl基因（ybhE ORF）编码的6-磷酸葡糖淀粉酶的活性而增加。
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3.发明申请Method for producing L-amino acids using bacteria of the Enterobacteriaceae family 有权使用肠杆菌科的细菌生产L-氨基酸的方法公开(公告)号：US20060088919A1
公开(公告)日：2006-04-27
申请号：US11247138
申请日：2005-10-12
申请人： Konstantin Rybak , Ekaterina Slivinskaya , Ekaterina Savrasova , Valeriy Akhverdian , Elena Klyachko , Sergei Mashko , Vera Doroshenko , Larisa Airikh , Tatyana Leonova , Mikhail Gusyatiner , Elvira Voroshilova , Yury Kozlov , Yoshihiko Hara , Takuji Ueda
发明人： Konstantin Rybak , Ekaterina Slivinskaya , Ekaterina Savrasova , Valeriy Akhverdian , Elena Klyachko , Sergei Mashko , Vera Doroshenko , Larisa Airikh , Tatyana Leonova , Mikhail Gusyatiner , Elvira Voroshilova , Yury Kozlov , Yoshihiko Hara , Takuji Ueda
摘要： There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.
摘要翻译： 公开了使用肠杆菌科的细菌生产L-氨基酸的方法，例如L-苏氨酸，L-赖氨酸，L-组氨酸，L-苯丙氨酸，L-精氨酸或L-谷氨酸，其中细菌 已被修饰以增强D-木糖通透酶的活性。
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4.发明申请Amino acid producing strains belonging to the genus Escherichia and a method for producing an amino acid 有权属于埃希氏杆菌属的氨基酸生产菌株和氨基酸生产方法公开(公告)号：US20060030009A1
公开(公告)日：2006-02-09
申请号：US11204011
申请日：2005-08-16
CPC分类号： C12N9/1205 , C12N15/52 , C12P13/04
摘要： Amino acids such as threonine, homoserine, isoleucine, lysine, valine and tryptophan are produced using a bacterium belonging to the genus Escherichia which has been constructed from a sucrose non-assimilative strain belonging to the genus Escherichia and which harbors sucrose non-PTS or PTS genes and has an ability to produce the amino acid.
摘要翻译： 氨基酸如苏氨酸，高丝氨酸，异亮氨酸，赖氨酸，缬氨酸和色氨酸使用属于埃希氏杆菌属的细菌产生，该细菌由属于埃希氏杆菌属的蔗糖非同化菌株构建，并含有蔗糖非PTS或PTS 基因并具有产生氨基酸的能力。
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