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    Method of producing L-amino acid
    1.
    发明授权
    Method of producing L-amino acid 有权
    生产L-氨基酸的方法

    公开(公告)号：US08512987B2

    公开(公告)日：2013-08-20

    申请号：US12545966

    申请日：2009-08-24

    申请人： Yuri Nagai ,  Kazuyuki Hayashi ,  Takuji Ueda ,  Yoshihiro Usuda ,  Kazuhiko Matsui

    发明人： Yuri Nagai ,  Kazuyuki Hayashi ,  Takuji Ueda ,  Yoshihiro Usuda ,  Kazuhiko Matsui

    IPC分类号： C12P13/24

    CPC分类号： C12P13/08 ,  C12P13/04

    摘要： An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.

    摘要翻译： 通过在含有甘油作为碳源的培养基中培养属于具有L-氨基酸生产能力的肠杆菌科的微生物并进行修饰以使甘油脱氢酶和二羟基丙酮激酶活性增加的L-氨基酸产生， 在培养基或细胞中积累L-氨基酸，并从培养基或细胞中收集L-氨基酸。

    Method for determining metabolic flux affecting substance production
    4.
    发明申请
    Method for determining metabolic flux affecting substance production 有权
    测定代谢通量影响物质生产的方法

    公开(公告)号：US20060154289A1

    公开(公告)日：2006-07-13

    申请号：US11337580

    申请日：2006-01-24

    申请人： Stephen Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Takuji Ueda ,  Yuichiro Tsuji

    发明人： Stephen Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Takuji Ueda ,  Yuichiro Tsuji

    IPC分类号： C12Q1/68 ,  C12Q1/18 ,  C12N1/21

    CPC分类号： C12N9/0006 ,  C12P13/08

    摘要： A metabolic flux affecting substance production using cells is determined by 1) creating a stoichiometric matrix based on formulas of biochemical reactions from a substrate through a desired produced substance, 2) selecting the same number of independent metabolic fluxes from all metabolic fluxes as the degree of freedom of the stoichiometric matrix as free fluxes, 3) creating a sufficient number of random combinations of the free fluxes for a statistical analysis and calculating a metabolic flux distribution from each created combination based on the stoichiometric matrix, 4) obtaining a regression equation including a minimum number of free fluxes that shows a correlation with substance production from the calculated metabolic flux distributions by a multivariate statistical analysis, and 5) determining at least one metabolic flux affecting substance production based on a coefficient in the obtained regression equation.

    摘要翻译： 影响使用细胞的物质生产的代谢通量是通过以下方法确定的：1）根据基质通过所需生产物质的生物化学反应的公式产生化学计量矩阵，2）从所有代谢通量中选择相同数量的独立代谢通量， 作为自由通量的化学计量矩阵的自由度，3）产生足够数量的自由通量的随机组合用于统计分析，并且基于化学计量矩阵计算每个创建的组合的代谢通量分布，4）获得包括 通过多变量统计分析从计算的代谢通量分布中显示与物质生产相关的最小数量的自由通量，以及5）基于所获得的回归方程中的系数确定影响物质生产的至少一种代谢通量。

    TABLET MANUFACTURING METHOD
    7.
    发明申请
    TABLET MANUFACTURING METHOD 审中-公开

    公开(公告)号：US20120319328A1

    公开(公告)日：2012-12-20

    申请号：US13582395

    申请日：2011-03-03

    申请人： Shinji Ando ,  Kenji Yoshida ,  Takuji Ueda

    发明人： Shinji Ando ,  Kenji Yoshida ,  Takuji Ueda

    IPC分类号： B29C43/02

    CPC分类号： A61J3/10 ,  B30B11/02 ,  B30B15/022

    摘要： An object is to provide a tablet manufacturing method which may restrain formation of a tablet edge surface and permits less effect of intra-tablet stress developed in process of press-forming on a tablet quality in cases where an upper rod and a lower rod are fittingly inserted into a vertical hole formed in a die to press powder in the die hole with a pushing surface at the lower end of the upper rod and a pushing surface at the upper end of the lower rod in order to press-form a tablet.Disclosed is a tablet manufacturing method in which an upper rod 3 and a lower rod 4 are fittingly inserted into a vertical hole 2 formed in a die 1 to press powder 5 in the die hole 2 with a pushing surface 9a at the lower end of the upper rod 3 and a pushing surface 11a at the upper end of the lower rod 4 in order to press-form a tablet 6, characterized in that concave forming grooves 2a integrally linked to the upper and lower pushing surfaces 9a and 11a are formed annularly in the inner circumferential surface of the die hole 2, and the die may be divided into an upper separable piece 7 and a lower separable piece 8 at the bottom portions of the forming grooves 2a, wherein after a final fixed rod or one of the upper and lower rods 3 and 4 is moved to and then fixed at a press-forming position at which the pushing surface 9a or 11a is integrally linked to the corresponding forming groove 2a, a final pressing rod or the other rod 3 or 4 is moved to the press-forming position so that the tablet 6 of a circular, elliptical or oval form as viewed in cross sectional side is press-formed, and then, the press-formed tablet 6 held in the forming grooves 2a is taken out of the die by separating the upper and lower separable pieces 7 and 8, in which case, the tablet 6 after being taken out of a final fixed separable piece or the separable piece 7 or 8 adjacent to the final fixed rod and also out of the final fixed rod is taken out of a final pressing separable piece or the separable piece 7 or 8 adjacent to the final pressing rod.