公开(公告)号：US5846842A

公开(公告)日：1998-12-08

申请号：US640141

申请日：1996-04-30

申请人： James N. Herron ,  Douglas A. Christensen ,  Karin D. Caldwell ,  Vera Janatova ,  Shao-Chie Huang ,  Hsu-Kun Wang

发明人： James N. Herron ,  Douglas A. Christensen ,  Karin D. Caldwell ,  Vera Janatova ,  Shao-Chie Huang ,  Hsu-Kun Wang

IPC分类号： G01N21/25 ,  G01N21/55 ,  G01N21/64 ,  G01N21/77 ,  G01N33/543 ,  G01N33/552

CPC分类号： G01N21/648 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/7703 ,  G01N33/54373 ,  Y10S435/808 ,  Y10S436/805

摘要： Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide and optionally has multi-well features and improved evanescent field intensity. The preferred biosensor and assay method have the capture molecules immobilized to the waveguide surface by site-specific coupling chemistry. Additionally, the coatings used to immobilize the capture molecules provide reduced non-specific protein adsorption.

公开(公告)号：US5677196A

公开(公告)日：1997-10-14

申请号：US263522

申请日：1994-06-22

申请人： James N. Herron ,  Douglas A. Christensen ,  Hsu-Kun Wang ,  Karin D. Caldwell ,  Vera Janatova ,  Shao-Chie Huang

发明人： James N. Herron ,  Douglas A. Christensen ,  Hsu-Kun Wang ,  Karin D. Caldwell ,  Vera Janatova ,  Shao-Chie Huang

IPC分类号： C08G65/329 ,  G01N21/64 ,  G01N21/77 ,  G01N33/543 ,  G01N33/552

CPC分类号： G01N21/7703 ,  C08G65/329 ,  G01N21/6428 ,  G01N21/648 ,  G01N2021/7786 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/809

摘要： Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide with an integral semi-cylindrical lens, and has multi-analyte features and calibration features, along with improved evanescent field intensity. A preferred embodiment of the biosensor and assay method have patches of capture molecules each specific for a different analyte disposed adjacent within a single reservoir. The capture molecules are immobilized to the patches on the waveguide surface by site-specific coupling of thiol groups on the capture molecules to photo-affinity crosslinkers which in turn are coupled to the waveguide surface or to a non-specific-binding-resistant coating on the surface. The patches of different antibodies are produced by selectively irradiating a portion of the waveguide surface during the process of coupling the photo-affinity crosslinkers the selective irradiation involving a mask, a laser light source, or the like.

摘要翻译： 公开了消逝光荧光免疫测定法的方法和装置。 该装置采用具有整体半柱面透镜的平面波导，并具有多分析物特征和校准特征，以及改善的消逝场强度。 生物传感器和测定方法的优选实施方案具有每个针对不同分析物特异性的捕获分子的斑块，其邻近设置在单个贮存器内。 捕获分子通过捕获分子上的硫醇基团的特异性偶联而固定在波导表面上的贴片上，而光致亲和性交联剂又连接到波导表面或非特异性结合抗性涂层上 表面。 通过在涉及光亲和性交联剂的涉及掩模，激光光源等的选择性照射的过程中选择性地照射波导表面的一部分来产生不同抗体的斑块。

公开(公告)号：US5512492A

公开(公告)日：1996-04-30

申请号：US64608

申请日：1993-05-18

申请人： James N. Herron ,  Douglas A. Christensen ,  Karin D. Caldwell ,  Vera Janatova ,  Shao-Chie Huang ,  Hsu-Kun Wang

发明人： James N. Herron ,  Douglas A. Christensen ,  Karin D. Caldwell ,  Vera Janatova ,  Shao-Chie Huang ,  Hsu-Kun Wang

IPC分类号： G01N21/25 ,  G01N21/55 ,  G01N21/64 ,  G01N21/77 ,  G01N33/543 ,  G01N33/544 ,  G01N33/552

CPC分类号： G01N21/648 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/7703 ,  G01N33/54373 ,  Y10S435/808 ,  Y10S436/805

摘要： Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide and optionally has multi-well features and improved evanescent field intensity. The preferred biosensor and assay method have the capture molecules immobilized to the waveguide surface by site-specific coupling chemistry. Additionally, the coatings used to immobilize the capture molecules provide reduced non-specific protein adsorption.

摘要翻译： 公开了消逝光荧光免疫测定法的方法和装置。 该装置采用平面波导并且可选地具有多阱特征和改善的消逝场强度。 优选的生物传感器和测定方法通过位点特异性偶联化学将捕获分子固定在波导表面上。 另外，用于固定捕获分子的涂层提供降低的非特异性蛋白质吸附。

公开(公告)号：US5919712A

公开(公告)日：1999-07-06

申请号：US748687

申请日：1996-11-13

申请人： James N. Herron ,  Douglas A. Christensen ,  Hsu-Kun Wang ,  Karin D. Caldwell ,  Vera Janatova ,  Shao-Chie Huang

发明人： James N. Herron ,  Douglas A. Christensen ,  Hsu-Kun Wang ,  Karin D. Caldwell ,  Vera Janatova ,  Shao-Chie Huang

IPC分类号： C07C281/02 ,  C08G65/329 ,  G01N21/25 ,  G01N21/64 ,  G01N21/77 ,  G01N33/543 ,  G01N33/552

CPC分类号： G01N21/6452 ,  C07C281/02 ,  C08G65/329 ,  G01N21/6428 ,  G01N21/648 ,  G01N21/7703 ,  G01N33/54353 ,  G01N33/54373 ,  G01N33/54393 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/807

摘要： Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide with an integral semi-cylindrical lens, and has multi-analyte features and calibration features, along with improved evanescent field intensity. A preferred embodiment of the biosensor and assay method have patches of capture molecules each specific for a different analyte disposed adjacent within a single reservoir. The capture molecules are immobilized to the patches on the waveguide surface by site-specific coupling of thiol groups on the capture molecules to photo-affinity crosslinkers which in turn are coupled to the waveguide surface or to a non-specific-binding-resistant coating on the surface. The patches of different antibodies are produced by selectively irradiating a portion of the waveguide surface during the process of coupling the photo-affinity crosslinkers the selective irradiation involving a mask, a laser light source, or the like.