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    Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays
    4.
    发明授权
    Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays 失效
    多分析物均匀荧光免疫测定的装置和方法

    公开(公告)号：US5677196A

    公开(公告)日：1997-10-14

    申请号：US263522

    申请日：1994-06-22

    申请人： James N. Herron ,  Douglas A. Christensen ,  Hsu-Kun Wang ,  Karin D. Caldwell ,  Vera Janatova ,  Shao-Chie Huang

    发明人： James N. Herron ,  Douglas A. Christensen ,  Hsu-Kun Wang ,  Karin D. Caldwell ,  Vera Janatova ,  Shao-Chie Huang

    IPC分类号： C08G65/329 ,  G01N21/64 ,  G01N21/77 ,  G01N33/543 ,  G01N33/552

    CPC分类号： G01N21/7703 ,  C08G65/329 ,  G01N21/6428 ,  G01N21/648 ,  G01N2021/7786 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/809

    摘要： Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide with an integral semi-cylindrical lens, and has multi-analyte features and calibration features, along with improved evanescent field intensity. A preferred embodiment of the biosensor and assay method have patches of capture molecules each specific for a different analyte disposed adjacent within a single reservoir. The capture molecules are immobilized to the patches on the waveguide surface by site-specific coupling of thiol groups on the capture molecules to photo-affinity crosslinkers which in turn are coupled to the waveguide surface or to a non-specific-binding-resistant coating on the surface. The patches of different antibodies are produced by selectively irradiating a portion of the waveguide surface during the process of coupling the photo-affinity crosslinkers the selective irradiation involving a mask, a laser light source, or the like.

    摘要翻译： 公开了消逝光荧光免疫测定法的方法和装置。 该装置采用具有整体半柱面透镜的平面波导，并具有多分析物特征和校准特征，以及改善的消逝场强度。 生物传感器和测定方法的优选实施方案具有每个针对不同分析物特异性的捕获分子的斑块，其邻近设置在单个贮存器内。 捕获分子通过捕获分子上的硫醇基团的特异性偶联而固定在波导表面上的贴片上，而光致亲和性交联剂又连接到波导表面或非特异性结合抗性涂层上 表面。 通过在涉及光亲和性交联剂的涉及掩模，激光光源等的选择性照射的过程中选择性地照射波导表面的一部分来产生不同抗体的斑块。

    Metal-chelating surfacant
    7.
    发明授权
    Metal-chelating surfacant 失效
    金属螯合表面活性剂

    公开(公告)号：US06087452A

    公开(公告)日：2000-07-11

    申请号：US89022

    申请日：1998-06-02

    申请人： Russell Stewart ,  Karin D. Caldwell ,  Chih-hu Ho ,  Loren Limberis

    发明人： Russell Stewart ,  Karin D. Caldwell ,  Chih-hu Ho ,  Loren Limberis

    IPC分类号： C07K17/06 ,  C08F293/00 ,  C07K1/00 ,  C07K14/00 ,  C08L3/04 ,  C08L53/00

    CPC分类号： C07K17/06 ,  Y10T436/13

    摘要： A modified surfactant of the PLURONIC.TM.-type compound and method for manufacture is disclosed. The surfactant compound has at least one PEO block attached at a first end to at least one PPO block, with at least one of the PEO block having an organic metal-chelating end group (R) attached to a second end, the remaining PEO blocks having an unmodified hydroxyl group at the second end. The metal-chelating group is charged with an metal-ion. A protein with a metal-affinity tag, e.g., a histidine tag, is then complexed with the metal-chelating group to form a complex of the modified surfactant, the metal ion, and the protein. The modified surfactant may be adsorbed upon a hydrophobic surface, charged with a metal ion, and then complexed with a protein with a metal-affinity tag. A surface with specific activity toward proteins is the presented, which is suitable for applications where a protein is immobilized upon a surface to give the surface specific protein activity.

    摘要翻译： 公开了PLURONIC TM型化合物的改性表面活性剂及其制造方法。 表面活性剂化合物在至少一个PPO嵌段的至少一个PPO嵌段上具有至少一个PEO嵌段，至少一个PEO嵌段具有连接到第二末端的有机金属螯合端基（R），其余的PEO嵌段 在第二端具有未改性的羟基。 在金属螯合基团上加入金属离子。 然后将具有金属亲和标签的蛋白质，例如组氨酸标签与金属螯合基团复合，形成修饰的表面活性剂，金属离子和蛋白质的复合物。 改性表面活性剂可以吸附在疏水性表面上，带有金属离子，然后与金属亲和标签的蛋白质复合。 提出了对蛋白质具有特异活性的表面，其适用于将蛋白质固定在表面上以产生表面特异性蛋白质活性的应用。