中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

15 records (took 0.017 seconds)

    End group activated polymers with oligonucleotide ligands
    4.
    发明授权
    End group activated polymers with oligonucleotide ligands 有权
    端基活化聚合物与寡核苷酸配体

    公开(公告)号：US07645721B2

    公开(公告)日：2010-01-12

    申请号：US10470296

    申请日：2002-02-04

    Applicant: Karin D. Caldwell ,  Jennifer A. Neff

    Inventor： Karin D. Caldwell ,  Jennifer A. Neff

    IPC: C40B50/18

    CPC classification number: C12N11/06

    Abstract: A method for coimmobilizing two or more biomolecules on a substrate in a defined ratio is disclosed. The method uses a copolymer conjugated to a number, N, of different types of oligonucleotides. The copolymer can be adsorbed to the surface of the substrate. N types of oligonucleotides complementary to the copolymer-bound oligonucleotides can be conjugated to N types of biomolecules. The types of the copolymer-bound oligonucleotides can be mixed in a defined ratio then adsorbed to the surface. The biomolecule-bound complementary oligonucletides can be conjugated to the copolymer-oligonucleotides to create a substrate with the biomolecules coimmobilized in a defined ratio. The invention also relates to a substrate prepared by the method of the invention.

    Abstract translation: 公开了一种以规定的比例将两种或更多种生物分子以规定比例共轭化的方法。 该方法使用与N个不同类型的寡核苷酸缀合的共聚物。 共聚物可以吸附到基材的表面。 与共聚物结合的寡核苷酸互补的N型寡核苷酸可以与N型生物分子缀合。 共聚物结合的寡核苷酸的类型可以以确定的比例混合，然后吸附到表面。 生物分子结合的互补寡核苷酸可以与共聚物 - 寡核苷酸缀合以产生具有以规定比例共聚的生物分子的底物。 本发明还涉及通过本发明的方法制备的底物。

    Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays
    6.
    发明授权
    Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays 失效
    多分析物均匀荧光免疫测定的装置和方法

    公开(公告)号：US5677196A

    公开(公告)日：1997-10-14

    申请号：US263522

    申请日：1994-06-22

    Applicant: James N. Herron ,  Douglas A. Christensen ,  Hsu-Kun Wang ,  Karin D. Caldwell ,  Vera Janatova ,  Shao-Chie Huang

    Inventor： James N. Herron ,  Douglas A. Christensen ,  Hsu-Kun Wang ,  Karin D. Caldwell ,  Vera Janatova ,  Shao-Chie Huang

    IPC: C08G65/329 ,  G01N21/64 ,  G01N21/77 ,  G01N33/543 ,  G01N33/552

    CPC classification number: G01N21/7703 ,  C08G65/329 ,  G01N21/6428 ,  G01N21/648 ,  G01N2021/7786 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/809

    Abstract: Methods and apparatus for evanescent light fluoroimmunoassays are disclosed. The apparatus employs a planar waveguide with an integral semi-cylindrical lens, and has multi-analyte features and calibration features, along with improved evanescent field intensity. A preferred embodiment of the biosensor and assay method have patches of capture molecules each specific for a different analyte disposed adjacent within a single reservoir. The capture molecules are immobilized to the patches on the waveguide surface by site-specific coupling of thiol groups on the capture molecules to photo-affinity crosslinkers which in turn are coupled to the waveguide surface or to a non-specific-binding-resistant coating on the surface. The patches of different antibodies are produced by selectively irradiating a portion of the waveguide surface during the process of coupling the photo-affinity crosslinkers the selective irradiation involving a mask, a laser light source, or the like.

    Abstract translation: 公开了消逝光荧光免疫测定法的方法和装置。 该装置采用具有整体半柱面透镜的平面波导，并具有多分析物特征和校准特征，以及改善的消逝场强度。 生物传感器和测定方法的优选实施方案具有每个针对不同分析物特异性的捕获分子的斑块，其邻近设置在单个贮存器内。 捕获分子通过捕获分子上的硫醇基团的特异性偶联而固定在波导表面上的贴片上，而光致亲和性交联剂又连接到波导表面或非特异性结合抗性涂层上 表面。 通过在涉及光亲和性交联剂的涉及掩模，激光光源等的选择性照射的过程中选择性地照射波导表面的一部分来产生不同抗体的斑块。

    Electrical field-flow fractionation using redox couple added to carrier fluid
    7.
    发明授权
    Electrical field-flow fractionation using redox couple added to carrier fluid 失效
    使用添加到载体流体中的氧化还原对电场流分级

    公开(公告)号：US5240618A

    公开(公告)日：1993-08-31

    申请号：US829970

    申请日：1992-02-03

    Applicant: Karin D. Caldwell ,  Yu-Shu Gao

    Inventor： Karin D. Caldwell ,  Yu-Shu Gao

    IPC: B01D17/06 ,  B01D21/00 ,  B03B5/00 ,  G01N30/00

    CPC classification number: B01D21/0009 ,  B01D17/06 ,  B03B5/00 ,  G01N30/0005 ,  G01N2030/0035 ,  G01N2030/005 ,  G01N2030/007

    Abstract: Improved apparatus and procedures for electrical field-flow fractionation is disclosed. A test sample is injected into the liquid carrier stream flowing through the flow channel of the apparatus. The apparatus comprises a thin flow channel having a top, a bottom, and two side walls, with a fluid carrier inlet at one end and an outlet at the other end. The top and bottom walls are formed such that at least the inner surfaces thereof that face the flow channel are made of an electrically conductive material so that the inner surfaces thereof form electrodes as well as opposite, broad boundary surfaces of the flow channel. A voltage differential is applied to the electrically conductive inner surfaces of the top and bottom walls. The carrier fluid flowing through the channel can be deionized or distilled water as well as water containing a red-ox couple such as quinone/hydroquinone.

    Abstract translation: 公开了用于电场流分级的改进的装置和程序。 将测试样品注入流过设备的流动通道的液体载体流中。 该装置包括具有顶部，底部和两个侧壁的薄流动通道，在一端具有流体载体入口，在另一端具有出口。 顶壁和底壁形成为使得至少其面向流动通道的内表面由导电材料制成，使得其内表面形成电极以及流动通道的相对的宽边界表面。 电压差被施加到顶壁和底壁的导电内表面。 流过通道的载体流体可以是去离子水或蒸馏水以及含有诸如醌/氢醌的红 - 氧对的水。

    DNA sequencing using fluorescence background electroblotting membrane
    8.
    发明授权
    DNA sequencing using fluorescence background electroblotting membrane 失效
    DNA序列使用荧光背景电泳膜

    公开(公告)号：US5112736A

    公开(公告)日：1992-05-12

    申请号：US365693

    申请日：1989-06-14

    Applicant: Karin D. Caldwell ,  Tun-Jen Chu ,  William G. Pitt

    Inventor： Karin D. Caldwell ,  Tun-Jen Chu ,  William G. Pitt

    IPC: B01D67/00 ,  C12Q1/68

    CPC classification number: B01D67/0093 ,  C12Q1/6869 ,  C12Q1/6874 ,  B01D2323/30 ,  Y10S435/805 ,  Y10S436/80 ,  Y10S436/807 ,  Y10T436/175383

    Abstract: A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through said smino groups contained on the surface thereof. The DNA fragments chemically bound to the membrane are subjected to hybridization probing with a tagged probe specific to the sequence of the DNA fragments. The tagging may be by either fluorophores or radioisotopes. The tagged probes hybridized to said target DNA fragments are detected and read by laser induced fluorescence detection or autoradiograms. The use of aminated low fluorescent background membranes allows the use of fluorescent detection and reading even when the available amount of DNA to be sequenced is small. The DNA bound to the membrances may be reprobed numerous times.

    Abstract translation: 公开了一种用于DNA上多重测序的方法，该方法包括已经通过凝胶电泳解析的电印迹或特异性碱基封端的DNA片段到已经表面改性的显示低背景荧光的中性非芳族聚合物微孔膜的表面上 含有氨基。 聚丙烯膜是优选的，氨基的引入是通过在胺化剂，优选氨的存在下使膜进行无线电或微波频率等离子体放电来实现的。 然后在电印迹之后在其表面上含有物理吸附的DNA片段的膜用诸如UV辐射或戊二醛喷雾剂的交联手段进行处理，以通过其表面上包含的所述唾液酸基将DNA片段化学结合到膜上。 用与DNA片段序列特异的标记探针对化学键合到膜上的DNA片段进行杂交探测。 标记可以是荧光团或放射性同位素。 通过激光诱导荧光检测或放射自显影检测和读取与所述靶DNA片段杂交的标记探针。 使用胺化的低荧光背景膜允许使用荧光检测和阅读，即使待测DNA的可用量很小。 与膜结合的DNA可以重复多次。

    Micromachined electrical field-flow fractionation system
    10.
    发明授权
    Micromachined electrical field-flow fractionation system 有权
    微加工电场流分离系统

    公开(公告)号：US6136171A

    公开(公告)日：2000-10-24

    申请号：US156151

    申请日：1998-09-18

    Applicant: A. Bruno Frazier ,  Karin D. Caldwell ,  Bruce K. Gale

    Inventor： A. Bruno Frazier ,  Karin D. Caldwell ,  Bruce K. Gale

    IPC: B03C5/02 ,  B81B1/00 ,  B81C1/00 ,  G01N30/00 ,  G01N27/26 ,  G01N27/447

    CPC classification number: G01N30/0005 ,  B03C5/026 ,  B81B1/00 ,  B81C1/00357 ,  B81C2201/019 ,  G01N2030/0035

    Abstract: A micromachined system for electrical field-flow fractionation of small test fluid samples is provided. The system includes a microchannel device comprising a first substrate having a planar inner surface with an electrode formed thereon. A second substrate having a planar inner surface with an electrode formed thereon is positioned over the first substrate so that the respective electrodes face each other. An insulating intermediate layer is interposed between the first and second substrates. The intermediate layer is patterned to form opposing sidewalls of at least one microchannel, with the electrodes on the substrates defining opposing continuous boundaries along the length of the microchannel. Inlet and outlet ports are formed in one or both substrates for allowing fluid flow into and out of the microchannel. The microchannel device can be fabricated with single or multiple microchannels therein for processing single or multiple test fluids. During operation, a voltage differential is applied to the electrodes in order to induce an electric field across the microchannel. This separates particles of different types present in a fluid injected into the microchannel. The separated particles in the fluid can be collected or further processed as desired.

    Abstract translation: 提供了一种用于小测试流体样品的电场分流的微加工系统。 该系统包括微通道装置，该微通道装置包括具有形成在其上的电极的平面内表面的第一基板。 具有形成有电极的平面内表面的第二基板位于第一基板上方，使得各个电极彼此面对。 绝缘中间层介于第一和第二基板之间。 图案化中间层以形成至少一个微通道的相对的侧壁，衬底上的电极沿着微通道的长度限定相对的连续边界。 入口和出口形成在一个或两个基板中，以允许流体流入和流出微通道。 微通道器件可以在其中制造单个或多个微通道，用于处理单个或多个测试流体。 在操作期间，电压差被施加到电极，以便引起跨过微通道的电场。 这样分离出注入到微通道中的流体中存在的不同类型的颗粒。 可以根据需要收集或进一步处理流体中分离的颗粒。

Patent Agency Ranking