公开(公告)号：US5466576A

公开(公告)日：1995-11-14

申请号：US86993

申请日：1993-07-02

申请人： Vincent P. Schulz ,  Virginia A. Zakian

发明人： Vincent P. Schulz ,  Virginia A. Zakian

IPC分类号： C12N9/90 ,  C12N15/54 ,  C12Q1/25 ,  C12Q1/68 ,  C12N9/00 ,  C12N9/10

CPC分类号： C12Q1/25 ,  C12N9/90

摘要： Method for affecting viability of a eucaryotic cell by contacting the cell with a modulator of the activity of a PIF-1-type helicase in the cell. Such contacting specifically increases or decreases the specific activity of the helicase in the cell.

摘要翻译： 通过使细胞与细胞中PIF-1型解旋酶活性的调节剂接触来影响真核细胞的存活力的方法。 这种接触特异性地增加或降低细胞中解旋酶的比活性。

公开(公告)号：US06232076B1

公开(公告)日：2001-05-15

申请号：US09498270

申请日：2000-02-04

申请人： Vincent P. Schulz

发明人： Vincent P. Schulz

IPC分类号： C12Q168

CPC分类号： C12Q1/6846 ,  C12Q1/6848 ,  C12Q2527/125

摘要： Methods for reducing degradation of polymerase extension products in the presence of formamide, where the polymerase extension products comprise a nonradioactive detection moiety, are disclosed. The methods comprise adding a base, a buffer or a reducing agent to the polymerase extension products. Sequencing methods which generate polymerase extension products comprising a nonradioactive detection moiety are also disclosed, wherein the degradation of the extension products is reduced or eliminated by the addition of a base, a buffer, or a reducing agent. Compositions for reducing degradation of the above polymerase extension products are also disclosed, where the compositions comprise a base, a buffer, or a reducing agent. Kits for performing sequencing methods are also disclosed, wherein the degradation of polymerase extension products is reduced or eliminated. The kits comprise a base, a buffer, or a reducing agent, and instructions.

摘要翻译： 公开了在甲酰胺存在下降低聚合酶延伸产物降解的方法，其中聚合酶延伸产物包含非放射性检测部分。 所述方法包括向聚合酶延伸产物中加入碱，缓冲液或还原剂。 还公开了产生包含非放射性检测部分的聚合酶延伸产物的测序方法，其中通过加入碱，缓冲液或还原剂降低或消除延伸产物的降解。 还公开了用于降低上述聚合酶延伸产物降解的组合物，其中组合物包含碱，缓冲液或还原剂。 还公开了用于进行测序方法的试剂盒，其中降低或消除了聚合酶延伸产物的降解。 试剂盒包括碱，缓冲液或还原剂和说明书。

公开(公告)号：US06476193B1

公开(公告)日：2002-11-05

申请号：US09167206

申请日：1998-10-06

申请人： Krishnan Nandabalan ,  Vincent P. Schulz ,  Meija Yang

发明人： Krishnan Nandabalan ,  Vincent P. Schulz ,  Meija Yang

IPC分类号： C07K1400

CPC分类号： C12N9/1205 ,  A01K2217/05 ,  A01K2217/075 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4738 ,  C07K2319/00

摘要： The present invention discloses complexes of the Nlk1 protein with proteins identified as interacting with the Nlk1 protein (Nlk1 protein-IPs) by a modified, improved yeast two hybrid assay system. The proteins which were identified to interact with the Nlk1 protein, and thus form complexes, included: TrkA, protein phosphatase 1&agr;, 14-3-3&egr;, &agr;-tropomyosin, vimentin, p0071, Ini-1, IP-1, IP-2, IP-3, IP-4 and IP-5, as well as derivatives, fragments analogs and homologs thereof. Methodologies of screening these aforementioned complexes for efficacy in treating and/or preventing various diseases and disorders, particularly, neoplasia, neurodegenerative disease, hypertrophic cardiomyopathy, viral infections and metabolic diseases and disorders are also disclosed herein.

摘要翻译： 本发明公开了通过修饰的改进的酵母双杂交测定系统将Nlk1蛋白与被鉴定为与Nlk1蛋白（Nlk1蛋白-IPs）相互作用的蛋白质的复合物。 被鉴定为与Nlk1蛋白相互作用，从而形成复合物的蛋白质包括：TrkA，蛋白磷酸酶1α，14-3-3eps，α-原肌球蛋白，波形蛋白，p0071，Ini-1，IP-1，IP-2 ，IP-3，IP-4和IP-5，以及其衍生物，片段类似物和同系物。 本文还公开了筛选这些上述复合物用于治疗和/或预防各种疾病和病症，特别是瘤形成，神经变性疾病，肥大性心肌病，病毒感染和代谢性疾病和病症的方法。