公开(公告)号：US5597704A

公开(公告)日：1997-01-28

申请号：US431962

申请日：1995-05-01

申请人： Yun-Huey Lee ,  Wen-Shen Chu ,  Wen-Hwei Hsu

发明人： Yun-Huey Lee ,  Wen-Shen Chu ,  Wen-Hwei Hsu

IPC分类号： C12P35/06 ,  C12Q1/26 ,  C12P35/00 ,  C12Q1/00 ,  C12Q1/02

CPC分类号： C12P35/06

摘要： A method of converting cephalosporin C to glutaryl-7-aminocephalosporanic acid. The method including obtaining a cell preparation from a microorganism which produces D-amino acid oxidase, the preparation being a cell-free extract or a suspension of permeated cells; adding a D-.alpha.-amino acid to the preparation; after the adding step, heating the preparation at 50.degree.-75.degree. C. for 5-60 minutes; and incubating cephalosporin C in the preparation. Also disclosed are a method of converting cephalosporin C to glutaryl-7-aminocephalosporanic acid in the absence of exogenous H.sub.2 O.sub.2, and a method of screening for a D-amino acid oxidase-producing microorganism.

摘要翻译： 将头孢菌素C转化为戊二酰-7-氨基头孢烷酸的方法。 该方法包括从产生D-氨基酸氧化酶的微生物获得细胞制剂，所述制剂是无细胞提取物或渗透细胞的悬浮液; 向制剂中加入D-氨基酸; 加入步骤后，在50-75℃加热制剂5-60分钟; 并在准备中孵育头孢菌素C。 还公开了在没有外源性H 2 O 2的情况下将头孢菌素C转化为戊二酰-7-氨基头孢烷酸的方法，以及筛选产生D-氨基酸氧化酶的微生物的方法。

公开(公告)号：US06403357B1

公开(公告)日：2002-06-11

申请号：US09836992

申请日：2001-04-18

申请人： Wen-Hwei Hsu ,  Chao-Hung Kao

发明人： Wen-Hwei Hsu ,  Chao-Hung Kao

IPC分类号： C12N986

CPC分类号： C12P13/04 ,  C12N9/86 ,  C12N15/70 ,  C12P41/009

摘要： The invention discloses a novel thermostable D-hydantoinase, and relates to the nucleic acid sequence, amino acid sequence and vector constructs of the enzyme. The thermostable D-hydantoinase of the invention shows about 45%-70% identity in amino acid sequence with other D-hydantoinases. The thermostable D-hydantoinase of the invention converts 5′-substituted D-hydantoinase to the corresponding N-carbamoyl-D- and/or -L-&agr;/&bgr;-amino acids, and retains at least 50% activity after 30 days at 50° C. In addition, the enzyme activity can also enhanced by certain divalent cations.

摘要翻译： 本发明公开了一种新型热稳定性D-乙内酰脲酶，涉及该酶的核酸序列，氨基酸序列和载体构建体。 本发明的热稳定性D-乙内酰脲酶在氨基酸序列中与其他D-乙内酰脲显示约45％-70％的同一性。 本发明的耐热性D-乙内酰脲将5'-取代的D-乙内酰脲转化成相应的N-氨基甲酰基-D-和/或-L-α/β-氨基酸，并且在50天后保持至少50％的活性 此外，酶活性也可以通过某些二价阳离子增强。

公开(公告)号：US5811277A

公开(公告)日：1998-09-22

申请号：US391769

申请日：1995-02-21

申请人： Tsuei Yun Fang ,  Long Liu Lin ,  Wen Hwei Hsu

发明人： Tsuei Yun Fang ,  Long Liu Lin ,  Wen Hwei Hsu

IPC分类号： C12N9/44 ,  C12N9/24

CPC分类号： C12N9/246 ,  C12Y302/01068

摘要： The present invention relates to a method for recovery and purification of isoamylase by raw starch adsorption method. Various raw starches are used to recover the isoamylase produced by a bacterium such as Pseudomonas amyloderamosa. The method comprises using raw starch to adsorb isoamylase under conditions suitable for such adsorption and isolating the adsorbed enzyme. The isoamylase adsorption rate varied with the species of raw starch. Additionally, raw starch can be recycled.

摘要翻译： 本发明涉及通过生淀粉吸附法回收和纯化异淀粉酶的方法。 使用各种原料淀粉来回收由细菌如淀粉假单胞菌（Pseudomonas amyloderamosa）产生的异淀粉酶。 该方法包括使用生淀粉在适于吸附和分离吸附的酶的条件下吸附异淀粉酶。 异淀粉酶的吸附速率随生淀粉的种类而变化。 另外，可以回收生淀粉。