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    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein and method of producing optically active amino acid
    1.
    发明申请
    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein and method of producing optically active amino acid 失效
    编码乙内酰脲的DNA，编码N-氨基甲酰-L-氨基酸水解酶的DNA，重组DNA，转化细胞，产生蛋白质的方法和产生旋光氨基酸的方法

    公开(公告)号：US20060240532A1

    公开(公告)日：2006-10-26

    申请号：US11476572

    申请日：2006-06-29

    Applicant: Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

    Inventor： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

    IPC: C12P13/04 ,  C12P13/22 ,  C07H21/04 ,  C12N9/80 ,  C12N15/74 ,  C12N1/21

    CPC classification number: C12N9/80 ,  C12N9/86 ,  C12P13/04 ,  C12P41/009 ,  C12Y305/01077 ,  C12Y305/02002

    Abstract: The present invention provides a method of producing optically active amino acids from 5-substituted hydantoin by isolating a hydantoinase gene and an N-carbamyl-L-amino acid hydrolase gene involved in an ability to convert 5-substituted hydantoin or N-carbamylamino acid into optically active amino acids from a microorganism of the genus Microbacterium having the above ability and by improving gene amplification and transcriptional and translational activities thereby preparing a recombinant wherein the amount of the desired enzymes produced is increased. The hydantoinase gene is, for example, a DNA encoding for a protein having a hydantoinase activity, which has the nucleotide sequence set forth in SEQ ID NO:1 in the Sequence. The N-carbamyl-L-amino acid hydrolase gene is, for example, a DNA encoding for a protein having an N-carbamyl-L-amino acid hydrolase activity, which has the nucleotide sequence set forth in SEQ ID NO:3 in the Sequence.

    Abstract translation: 本发明提供了一种通过分离涉及将5-取代的乙内酰脲或N-氨基甲酰基氨基酸转化成5-取代的乙内酰脲或N-氨基甲酰基氨基酸的能力的乙内酰脲酶基因和N-氨基甲酰基-L-氨基酸水解酶基因从5-取代的乙内酰脲生产旋光性氨基酸的方法 具有上述能力的微杆菌属的微生物的旋光活性氨基酸，通过改善基因扩增和转录和翻译活性，从而制备重组体，其中产生的所需酶的量增加。 乙内酰脲酶基因是例如编码具有乙内酰脲酶活性的蛋白质的DNA，其具有序列中SEQ ID NO：1所示的核苷酸序列。 N-氨基甲酰基-L-氨基酸水解酶基因是例如编码具有N-氨基甲酰基-L-氨基酸水解酶活性的蛋白质的DNA，其具有SEQ ID NO：3所示的核苷酸序列 序列。

    Process for producing optically active alpha -methylcysteine derivative
    4.
    发明申请
    Process for producing optically active alpha -methylcysteine derivative 审中-公开
    光学活性α-甲基半胱氨酸衍生物的制备方法

    公开(公告)号：US20060172393A1

    公开(公告)日：2006-08-03

    申请号：US10541556

    申请日：2003-12-08

    Applicant: Makoto Ueda ,  Hirokazu Nanba

    Inventor： Makoto Ueda ,  Hirokazu Nanba

    IPC: C12P13/12

    CPC classification number: C12N9/80 ,  C12P13/12 ,  C12P41/009

    Abstract: A process for conveniently and industrially producing an optically active α-methylcysteine derivative, which is useful as an intermediate of medicines and the like, from an inexpensive and readily available material is provided. The present invention relates to a process for producing a racemic or optically active α-methylcysteine derivative including a step of hydrolyzing a racemic or optically active N-carbamyl-α-methylcysteine derivative by treating with decarbamylase, and a process for producing an optically active α-methylcysteine derivative and an optically active N-carbamyl-α-methylcysteine derivative having a configuration opposite to that of the compound including a step of stereoselectively hydrolyzing a racemic N-carbamyl-α-methylcysteine derivative by treating with decarbamylase.

    Abstract translation: 提供了一种方便和工业地制造光学活性的α-甲基半胱氨酸衍生物的方法，其可用作药物等的中间体，由便宜且容易获得的材料。 本发明涉及一种外消旋或光学活性α-甲基半胱氨酸衍生物的制备方法，包括通过用脱氨基甲酰酶处理水解外消旋或光学活性的N-氨基甲酰基-α-甲基半胱氨酸衍生物的步骤，以及制备光学活性α - 甲基半胱氨酸衍生物和具有与包括通过用脱氨基甲酰酶处理立体选择性水解外消旋N-氨基甲酰基-α-甲基半胱氨酸衍生物的步骤的化合物相反的构型的光学活性N-氨基甲酰基-α-甲基半胱氨酸衍生物。

    Thermostable D-hydantoinase and the application thereof
    8.
    发明授权
    Thermostable D-hydantoinase and the application thereof 有权
    热稳定性D-乙内酰脲酶及其应用

    公开(公告)号：US06403357B1

    公开(公告)日：2002-06-11

    申请号：US09836992

    申请日：2001-04-18

    Applicant: Wen-Hwei Hsu ,  Chao-Hung Kao

    Inventor： Wen-Hwei Hsu ,  Chao-Hung Kao

    IPC: C12N986

    CPC classification number: C12P13/04 ,  C12N9/86 ,  C12N15/70 ,  C12P41/009

    Abstract: The invention discloses a novel thermostable D-hydantoinase, and relates to the nucleic acid sequence, amino acid sequence and vector constructs of the enzyme. The thermostable D-hydantoinase of the invention shows about 45%-70% identity in amino acid sequence with other D-hydantoinases. The thermostable D-hydantoinase of the invention converts 5′-substituted D-hydantoinase to the corresponding N-carbamoyl-D- and/or -L-&agr;/&bgr;-amino acids, and retains at least 50% activity after 30 days at 50° C. In addition, the enzyme activity can also enhanced by certain divalent cations.

    Abstract translation: 本发明公开了一种新型热稳定性D-乙内酰脲酶，涉及该酶的核酸序列，氨基酸序列和载体构建体。 本发明的热稳定性D-乙内酰脲酶在氨基酸序列中与其他D-乙内酰脲显示约45％-70％的同一性。 本发明的耐热性D-乙内酰脲将5'-取代的D-乙内酰脲转化成相应的N-氨基甲酰基-D-和/或-L-α/β-氨基酸，并且在50天后保持至少50％的活性 此外，酶活性也可以通过某些二价阳离子增强。

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