摘要:
A method of converting cephalosporin C to glutaryl-7-aminocephalosporanic acid. The method including obtaining a cell preparation from a microorganism which produces D-amino acid oxidase, the preparation being a cell-free extract or a suspension of permeated cells; adding a D-.alpha.-amino acid to the preparation; after the adding step, heating the preparation at 50.degree.-75.degree. C. for 5-60 minutes; and incubating cephalosporin C in the preparation. Also disclosed are a method of converting cephalosporin C to glutaryl-7-aminocephalosporanic acid in the absence of exogenous H.sub.2 O.sub.2, and a method of screening for a D-amino acid oxidase-producing microorganism.
摘要翻译:将头孢菌素C转化为戊二酰-7-氨基头孢烷酸的方法。 该方法包括从产生D-氨基酸氧化酶的微生物获得细胞制剂,所述制剂是无细胞提取物或渗透细胞的悬浮液; 向制剂中加入D-氨基酸; 加入步骤后,在50-75℃加热制剂5-60分钟; 并在准备中孵育头孢菌素C。 还公开了在没有外源性H 2 O 2的情况下将头孢菌素C转化为戊二酰-7-氨基头孢烷酸的方法,以及筛选产生D-氨基酸氧化酶的微生物的方法。
摘要:
A method of screening for a given enzyme in colony-forming cells using a substrate of the enzyme that has low solubility. Use of such a method leads to the discovery of glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase of Pseudomonas nitroreducens. Also disclosed is a method of obtaining GL-7-ACA acylase from Pseudomonas cells.
摘要:
Proteins isolated from Coprinus clastophyllus having prolyl oligopeptidase activity, nucleic acids encoding the protein and methods for producing and using the protein, wherein SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:9 and SEQ ID NO:10 must be contained therein to at least 60% similarity. The proteins and nucleic acids have improved heat stability and perform more favorably in vivo having optimum activity conditions around 40 degrees centigrade and around pH 7, and can therefore be used in medicaments for the treatment of celiac disease caused by proline abundant gluten or other applications.
摘要翻译:从具有脯氨酰寡肽酶活性的鬼马属分离得到的蛋白质,编码蛋白质的核酸及其制备和使用蛋白质的方法,其中SEQ ID NO:5,SEQ ID NO:6,SEQ ID NO:9和SEQ ID NO:10必须为 含有至少60%的相似性。 蛋白质和核酸具有改善的热稳定性,并且在体内具有最佳活性条件在约40摄氏度和约pH 7左右的体内更有利,因此可用于治疗由脯氨酸丰富的面筋或其他应用引起的乳糜泻的药物。
摘要:
A method of producing erythritol. The method includes growing in a culture an isolated yeast strain that possesses certain unique characteristics and is capable of converting glucose to erythritol in a glucose-containing medium, and purifying erythritol from the culture.
摘要:
Proteins isolated from Coprinus clostophyllus having prolyl oligopeptidase activity, nucleic acids encoding the protein and methods for producing and using the protein, wherein SEQ ID NO:5, SEQ ID NO:6 and SEQ ID NO:10 must be contained therein to at least 60% similarity. The proteins and nucleic acids have improved heat stability and perform more favorably in vivo having optimum activity conditions around 40 degrees centigrade and around pH 7, and can therefore be used in medicaments for the treatment of celiac disease caused by proline abundant gluten or other applications.
摘要翻译:分离自具有脯氨酰寡肽酶活性的鬼伞属的蛋白质,编码蛋白质的核酸及其生产和使用蛋白质的方法,其中必须含有SEQ ID NO:5,SEQ ID NO:6和SEQ ID NO:10至少至少60 %相似度。 蛋白质和核酸具有改善的热稳定性,并且在体内具有最佳活性条件在约40摄氏度和约pH 7左右的体内更有利,因此可用于治疗由脯氨酸丰富的面筋或其他应用引起的乳糜泻的药物。
摘要:
A DNA molecule encoding transglutaminase, a transglutaminase, an expression vector containing the DNA molecule, and a cell containing the expression vector.
摘要:
The invention provides a DNA molecule encoding transglutaminase of Streptoverticillium ladakanum, the encoded transglutaminase and the use of the transglutaminase in industrial process.
摘要:
This invention relates to the isolation of thermophilic phytase-producing microorganisms, method for producing phytase using such microorganisms, phytase obtained therefrom, and usage of the phytase to hydrolyze phytic acid or phytate. In particular, this invention relates to phytase-producing microorganisms, which belong to Streptomyces sp., Pseudonocardia sp. or Microbispora sp., and which produce phytase available for recovery in an efficient and practical manner.
摘要:
This invention relates to the isolation of thermophilic phytase-producing microorganisms, method for producing phytase using such microorganisms, phytase obtained therefrom, and usage of the phytase to hydrolyze phytic acid or phytate. In particular, this invention relates to phytase-producing microorganisms, which belong to Streptomyces sp., Pseudonocardia sp. or Microbispora sp., and which produce phytase available for recovery in an efficient and practical manner.