公开(公告)号：US4569790A

公开(公告)日：1986-02-11

申请号：US594223

申请日：1984-03-28

申请人： Kirston Koths ,  James Thomson ,  Michael Kunitani ,  Kenneth Wilson ,  Wolf Hanisch

发明人： Kirston Koths ,  James Thomson ,  Michael Kunitani ,  Kenneth Wilson ,  Wolf Hanisch

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P35/00 ,  C07K1/113 ,  C07K1/14 ,  C07K1/20 ,  C07K14/00 ,  C07K14/52 ,  C07K14/54 ,  C07K14/55 ,  C12N15/26 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C07K3/12

CPC分类号： C07K1/1133 ,  C07K14/55 ,  A61K38/00

摘要： A process for recovering microbially produced IL-2 in a highly pure form from the cellular material of the microorganisms that produced it comprising: disrupting the cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC.

公开(公告)号：US5419899A

公开(公告)日：1995-05-30

申请号：US879753

申请日：1992-05-06

申请人： Kirston Koths ,  James Thomson ,  Michael Kunitani ,  Kenneth Wilson ,  Wolf Hanisch

发明人： Kirston Koths ,  James Thomson ,  Michael Kunitani ,  Kenneth Wilson ,  Wolf Hanisch

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P35/00 ,  C07K1/113 ,  C07K1/14 ,  C07K1/20 ,  C07K14/00 ,  C07K14/52 ,  C07K14/54 ,  C07K14/55 ,  C12N15/26 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  A61K38/20

CPC分类号： C07K1/1133 ,  C07K14/55 ,  A61K38/00

摘要： A process for recovering microbially produced IL-2 in a highly pure form from the cellular material of the microorganisms that produced it comprising: disrupting the cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction, mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC.

摘要翻译： 从产生它的微生物的细胞材料中回收高纯度形式的微生物产生的IL-2的方法，包括：破坏微生物的细胞膜; 用诸如尿素的离液剂提取破坏物，其从细胞材料中选择性提取微生物蛋白质; 将IL-2溶解在提取的固相中，与含有还原剂的增溶剂如SDS的水溶液混合; 并通过任选用2-丁醇或2-甲基-2-丁醇萃取，然后用凝胶过滤层析，将IL-2与所得溶液分离，氧化IL-2并通过RP-HPLC纯化氧化的IL-2。

公开(公告)号：US5614185A

公开(公告)日：1997-03-25

申请号：US428895

申请日：1995-04-25

申请人： Kirston Koths ,  James Thomson ,  Michael Kunitani ,  Kenneth Wilson ,  Wolf Hanisch

发明人： Kirston Koths ,  James Thomson ,  Michael Kunitani ,  Kenneth Wilson ,  Wolf Hanisch

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P35/00 ,  C07K1/113 ,  C07K1/14 ,  C07K1/20 ,  C07K14/00 ,  C07K14/52 ,  C07K14/54 ,  C07K14/55 ,  C12N15/26 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  A61K38/20

CPC分类号： C07K1/1133 ,  C07K14/55 ,  A61K38/00

摘要： A process for recovering microbially produced IL-2 in a highly pure form from the cellular material of the microorganisms that produced it comprising: disrupting the-cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC.

摘要翻译： 从产生它的微生物的细胞材料中回收高纯度形式的微生物产生的IL-2的方法，包括：破坏微生物的细胞膜; 用诸如尿素的离液剂提取破坏物，其从细胞材料中选择性提取微生物蛋白质; 用含有还原剂的增溶剂如SDS的水溶液将IL-2溶解在萃取混合物的固相中; 并通过任选用2-丁醇或2-甲基-2-丁醇萃取，然后用凝胶过滤层析，将IL-2与所得溶液分离，氧化IL-2并通过RP-HPLC纯化氧化的IL-2。