摘要:
A process for recovering microbially produced IL-2 in a highly pure form from the cellular material of the microorganisms that produced it comprising: disrupting the cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction, mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC.
摘要:
A process for recovering microbially produced IL-2 in a highly pure form from the cellular material of the microorganisms that produced it comprising: disrupting the cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC.
摘要:
A process for recovering microbially produced IL-2 in a highly pure form from the cellular material of the microorganisms that produced it comprising: disrupting the-cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC.
摘要:
A wrapping apparatus for wrapping a sheet of a covering material from a roll onto an outer surface of a surgical site. The wrapping apparatus includes a handle and a body portion having a housing, a first end, and a second end. The housing and the first and second ends generally defining an interior region that is configured to receive the placement of the roll. The wrapping apparatus further includes first and second spindles that are configured to allow rotation of the roll relative to the handle for the dispensing of the sheet of covering material from the roll. The wrapping apparatus may also include a cap that is operably connected to the second end, and which secures the roll to the wrapping apparatus. Additionally, the wrapping apparatus may include a cutting apparatus that cuts or tears dispensed covering material away from the roll of covering material.
摘要:
A cache coherent distributed shared memory multi-processor computer system is provided with a memory controller which includes a recall unit. The recall unit allows selective forced write-backs of dirty cache lines to the home memory. After a request is posted in the recall unit, a recall (“flush”) command is issued which forces the owner cache to write-back the dirty cache line to be flushed. The memory controller will inform the recall unit as each recall operation is completed. The recall unit operation will be interrupted when all flush requests are completed.
摘要:
The present invention relates to a method and device for controlling the solid fuel regression rate of a solid fuel ramjet engine. In a preferred embodiment of the present invention, pressure oscillations are excited using active, closed-loop control to vary the heat transfer, and in turn the regression rate of a solid fuel ramjet engine. The pressure oscillations are excited by introducing small disturbances into the shear layer. The disturbances are amplified in the shear layer and excite pressure oscillations. The disturbances are introduced at a certain phase relative to the pressure oscillations. This is accomplished by closed-loop control. By varying the phase, the amplitude of the pressure oscillations can also be varied, which in turn will vary the heat transfer, regression rate, and thrust level.
摘要:
A tape head cleaner cartridge for receipt in a tape machine having a magnetic tape head and comprising a cartridge housing for supporting a cleaning pad that is supported by and operated from a lever. The lever may be manually operated to cause the cleaning pad to transition across the tape head in direction of tape travel. In an alternate embodiment of the invention, the cartridge supports both a magnetic head cleaning pad and a capstan cleaning pad. A dispenser is provided for a plurality of cleaning pads. A kit is provided for containing the cartridge, dispenser and cleaning fluid.
摘要:
A phase shifting device including a coupling device having an input port, an output port and a further port. The further port is connected to a transmission line, along the length of which are connected a number of switches. Each switch is operable to connect a low impedance across the transmission line such that an input signal applied to the input port is reflected at a connected one of the switches to produce an output signal at the output port whose phase is shifted relative to that of the input signal by an amount dependent on the length of transmission line traversed by the input signal. Alternatively the capacitance of each of the switches may be operated so as to vary the propagation constant of the transmission line to thereby produce the required phase shift.
摘要:
A sediment retention device including a water-permeable outer containment structure, a water-impermeable injection tube including an outlet disposed within the outer containment structure, and a flocculent treatment material disposed within the injection tube. The injection tube can include an agitation structure to promote mixing contaminated water with the treatment material. A baffle including water distribution openings can be disposed over the outlet to distribute water into the outer containment structure.
摘要:
A downhole activation system within a tubular. The system includes an axially movable mover. A first magnet attached to the mover. The first magnet axially movable with the mover. A second magnet separated from the first magnet. The second magnet magnetically repulsed by the first magnet. A biasing device urging the second magnet towards the first magnet; wherein movement of the first magnet via the mover towards the second magnet moves the second magnet in a direction against the biasing device. Also included is a method of activating an activatable member in a downhole tubular.