公开(公告)号：US5741411A

公开(公告)日：1998-04-21

申请号：US665800

申请日：1996-06-18

Applicant: Edward S. Yeung ,  Qingbo Li ,  Xiandan Lu

Inventor： Edward S. Yeung ,  Qingbo Li ,  Xiandan Lu

IPC: G01N27/447 ,  G01N27/26

CPC classification number: G01N27/44782 ,  G01N27/44721

Abstract: The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.

公开(公告)号：US5582705A

公开(公告)日：1996-12-10

申请号：US444565

申请日：1995-05-19

Applicant: Edward S. Yeung ,  Huan-Tsang Chang ,  Eliza N. Fung ,  Qingbo Li ,  Xiandan Lu

Inventor： Edward S. Yeung ,  Huan-Tsang Chang ,  Eliza N. Fung ,  Qingbo Li ,  Xiandan Lu

IPC: G01N27/447 ,  G01N27/26

CPC classification number: G01N27/44782 ,  G01N27/44721

Abstract: The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.

Abstract translation: 本发明提供了用于多路复用毛细管电泳系统中的侧入光激发几何形状。 电荷注入装置光学耦合到阵列中的毛细管，使得毛细管的内部成像到仅一个像素上。 在Sanger型4标记DNA测序反应中，通过使用两个长通滤波器将荧光发射分解成两个发射通道来改善核苷酸鉴定（“碱基呼叫”）。 在电泳分离中使用二元聚（环氧乙烷）基质。