公开(公告)号：US20090112487A1

公开(公告)日：2009-04-30

申请号：US12258398

申请日：2008-10-25

Applicant: Guangjun Zhang ,  Junhua Sun ,  Zhenzhong Wei ,  Fuqiang Zhou ,  Qingbo Li ,  Zhen Liu ,  Qianzhe Liu

Inventor： Guangjun Zhang ,  Junhua Sun ,  Zhenzhong Wei ,  Fuqiang Zhou ,  Qingbo Li ,  Zhen Liu ,  Qianzhe Liu

IPC: G01B11/00

CPC classification number: G01B11/2513 ,  B61K9/08 ,  G01B11/2522

Abstract: The invention discloses a vehicle dynamic measurement device for comprehensive parameters of rail wear, which comprises a vision sensor, a computer and a milometer. A high-speed image acquisition card and a measurement module are installed in the computer. The vision sensor comprises imaging system for rail cross-section and a raster projector which can project more than one light plane perpendicular to the measured rail. The measurement module is used for calculating vertical wear, horizontal wear, the amplitude and wavelength of corrugation wear. The invention also discloses a vehicle dynamic measurement method for comprehensive parameters of rail wear. The invention can increase the sampling rate of image sensing and acquisition hardware equipment with no need of improving the performance of it, thereby satisfy high-speed on-line dynamic measurement requirements for corrugation wear, and the amplitude and wavelength of corrugation wear can be calculated more precisely.

Abstract translation: 本发明公开了一种用于轨道磨损综合参数的车辆动态测量装置，其包括视觉传感器，计算机和千米。 计算机中安装有高速图像采集卡和测量模块。 视觉传感器包括用于轨道横截面的成像系统和光栅投影仪，该投影仪可以投影多个垂直于测量轨道的光平面。 测量模块用于计算垂直磨损，水平磨损，波纹磨损的振幅和波长。 本发明还公开了一种用于铁路磨损综合参数的车辆动态测量方法。 本发明可以增加图像感测和采集硬件设备的采样率，不需要提高其性能，从而满足波纹磨损的高速在线动态测量要求，并且可以计算波纹磨损的幅度和波长 更确切地说。

公开(公告)号：US07473341B2

公开(公告)日：2009-01-06

申请号：US10661558

申请日：2003-09-15

Applicant: Qingbo Li ,  Kevin J. Levan ,  Heidi Monroe

Inventor： Qingbo Li ,  Kevin J. Levan ,  Heidi Monroe

IPC: G01N27/447

CPC classification number: G01N27/44704 ,  G01N27/44747

Abstract: The present invention relates to a method of separating a sample comprising biological compounds, such as nucleic acids. The nucleic acids are subjected to electrophoresis using a matrix that is essentially free of denaturants and having at least one random, linear copolymer comprising a first comonomer of acrylamide and at least one secondary comonomer. A temperature of at least a portion of the matrix is at least about 80° C.

Abstract translation: 本发明涉及分离包含生物化合物如核酸的样品的方法。 使用基本上不含变性剂并具有至少一种包含丙烯酰胺的第一共聚单体和至少一种仲共聚单体的无规线性共聚物进行电泳的核酸。 基质的至少一部分的温度为至少约80℃

公开(公告)号：US20060091011A1

公开(公告)日：2006-05-04

申请号：US11204773

申请日：2005-08-15

Applicant: Changsheng Liu ,  Thomas Kane ,  Qingbo Li

Inventor： Changsheng Liu ,  Thomas Kane ,  Qingbo Li

IPC: C07K1/26 ,  G01N27/447

CPC classification number: C07K1/26 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782 ,  Y10T436/2575

Abstract: An automated capillary zone electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a container connected to the detection end of the capillaries. The container is provided with valving which facilitate cleaning the capillaries, loading buffer into the capillaries, introducing samples to be electrophoresced into the capillaries, and performing capillary zone electrophoresis on the thus introduced samples.

公开(公告)号：US06375819B1

公开(公告)日：2002-04-23

申请号：US09373909

申请日：1999-08-13

Applicant: Qingbo Li ,  Changsheng Liu ,  Thomas E. Kane ,  John R. Kernan ,  Bernard Sonnenschein ,  Michael V. Sharer

Inventor： Qingbo Li ,  Changsheng Liu ,  Thomas E. Kane ,  John R. Kernan ,  Bernard Sonnenschein ,  Michael V. Sharer

IPC: G01N2726

CPC classification number: G01N27/44721 ,  G01N27/44782

Abstract: An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.

Abstract translation: 公开了一种自动电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有含有凝胶注射器/步进电机或具有泵的高压室的凝胶递送模块，以通过毛细管快速均匀地输送凝胶。 该系统还包括多波长光束发生器，以产生产生具有宽波长范围的光束的激光束。 离线毛细管修复器彻底清洁毛细管柱，以便同时执行另一个毛细管柱的电泳。 离线毛细管再生器的流线型性质提供了增加系统吞吐量的优点，同时最小化了系统成本。

公开(公告)号：US07697976B2

公开(公告)日：2010-04-13

申请号：US10586272

申请日：2005-01-18

Applicant: Jinguang Wu ,  Yuanfu Zhang ,  Yizhuang Xu ,  Qingbo Li ,  Shifu Weng ,  Limin Yang ,  Li Zhang ,  Weijin Zhou ,  Zhanlan Yang ,  Weihong Li ,  Huizhong Wu ,  Rongye Gong ,  Xuejun Gao ,  Duanfu Xu ,  Ying Zhao ,  Zhi Xu ,  Nengwei Zhang ,  Lixin Wang ,  Kaihua Sun ,  Xin Peng ,  Yufeng Liu ,  Xuejun Sun ,  Xiaofeng Ling ,  Su Zhou ,  Zhi Liu ,  Guoqiao Lai ,  Jianxiong Jiang ,  Qinghua Pan ,  Qiguang Wu ,  Guangyan Yu ,  Bingbing Wang

Inventor： Jinguang Wu ,  Yuanfu Zhang ,  Yizhuang Xu ,  Qingbo Li ,  Shifu Weng ,  Limin Yang ,  Li Zhang ,  Weijin Zhou ,  Zhanlan Yang ,  Weihong Li ,  Huizhong Wu ,  Rongye Gong ,  Xuejun Gao ,  Duanfu Xu ,  Ying Zhao ,  Zhi Xu ,  Nengwei Zhang ,  Lixin Wang ,  Kaihua Sun ,  Xin Peng ,  Yufeng Liu ,  Xuejun Sun ,  Xiaofeng Ling ,  Su Zhou ,  Zhi Liu ,  Guoqiao Lai ,  Jianxiong Jiang ,  Qinghua Pan ,  Qiguang Wu ,  Guangyan Yu ,  Bingbing Wang

IPC: A61B5/02

CPC classification number: A61B5/0059 ,  A61B5/6848 ,  A61B5/7257

Abstract: The invention relates to a non-invasive method to diagnose the changes of molecular structures of organism tissues from body surface and a dedicated apparatus. The apparatus is comprised of a Fourier Transform infrared spectrometer and a set of additional accessories. Said additional accessories include a mid-IR fiber optics sampling attachment, a fiber coupling part, and an infrared detector part. The detection method is comprised of placing the ATR probe of the dedicated apparatus on the skin surface of a region to be tested, and scanning more than one time in which the resolution of the apparatus is 1-32 cm−1 and the range of the spectrum is 800-4000 cm−1. It is possible to detect changes in molecular structures of living biological tissues in the early stages of cancer, and testees will not feel uncomfortable during testing. The method is easy to operate, quick, accurate, and it doesn't harm the body.

Abstract translation: 本发明涉及用于诊断来自身体表面的生物体组织的分子结构的变化的非侵入性方法和专用设备。 该装置由傅里叶变换红外光谱仪和一组附加附件组成。 所述附加附件包括中红外光纤采样附件，光纤耦合部件和红外探测器部件。 检测方法包括将专用装置的ATR探针放置在要测试的区域的皮肤表面上，扫描多于一次，其中装置的分辨率为1-32cm-1，并且扫描范围 光谱为800-4000cm-1。 在癌症早期阶段可以检测生物体组织的分子结构变化，受试者在测试过程中不会感到不适。 该方法易于操作，快速，准确，不伤身体。

公开(公告)号：US07459070B2

公开(公告)日：2008-12-02

申请号：US11204773

申请日：2005-08-15

Applicant: Changsheng Liu ,  Thomas E. Kane ,  Qingbo Li

Inventor： Changsheng Liu ,  Thomas E. Kane ,  Qingbo Li

IPC: G01N27/447 ,  G01N27/453

CPC classification number: C07K1/26 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782 ,  Y10T436/2575

Abstract: An automated capillary zone electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a container connected to the detection end of the capillaries. The container is provided with valving which facilitate cleaning the capillaries, loading buffer into the capillaries, introducing samples to be electrophoresced into the capillaries, and performing capillary zone electrophoresis on the thus introduced samples.

Abstract translation: 公开了一种自动化毛细管区域电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有连接到毛细管的检测端的容器。 该容器设置有阀，其有助于清洁毛细管，将缓冲液加载到毛细管中，将待电泳的样品引入毛细管中，并在这样引入的样品上进行毛细管区带电泳。

公开(公告)号：US06704104B2

公开(公告)日：2004-03-09

申请号：US10221734

申请日：2002-09-16

Applicant: Qingbo Li

Inventor： Qingbo Li

IPC: G01N2164

CPC classification number: G01N21/6452 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00599 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/00722 ,  C40B40/06 ,  C40B50/08 ,  G01N21/6456 ,  G01N2021/6421 ,  G01N2021/6463

Abstract: The present invention relates to an array reader having a light source configured to emit an excitation light, a substrate comprising a plurality of sites spatially configured as a two-dimensional array having a plurality of rows and a plurality of columns, where each site is configured to support a sample. The array reader includes a changing device configured to determine which of said plurality of sites is illuminated at any given instant and a detector comprising a two-dimensional array of light sensitive elements, transmission grating beam splitter (TGBS) disposed along an optical path between the substrate and the detector, and a single light focusing element disposed along an optical path between the substrate and the detector. The TGBS is configured to receive non-collimated light emitted by at least one sample illuminated by said excitation light.

Abstract translation: 本发明涉及具有被配置为发射激发光的光源的阵列读取器，包括空间地配置为具有多行和多列的二维阵列的多个位置的基板，其中每个位置被配置 支持样本。 阵列读取器包括：变更装置，被配置为确定在任何给定时刻照射哪个所述多个位点;以及检测器，其包括光敏元件的二维阵列，沿着光路的光路设置的透射光栅分束器（TGBS） 基板和检测器，以及沿着基板和检测器之间的光路设置的单个光聚焦元件。 TGBS被配置为接收由所述激发光照射的至少一个样本发射的非准直光。

公开(公告)号：US06365024B1

公开(公告)日：2002-04-02

申请号：US09372151

申请日：1999-08-11

Applicant: Qingbo Li ,  Thomas E. Kane ,  Changsheng Liu ,  Bernard Sonnenschein ,  Michael V. Sharer ,  John R. Kernan

Inventor： Qingbo Li ,  Thomas E. Kane ,  Changsheng Liu ,  Bernard Sonnenschein ,  Michael V. Sharer ,  John R. Kernan

IPC: G01N2726

CPC classification number: G01N27/44721 ,  G01N27/44782

Abstract: An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.

Abstract translation: 公开了一种自动电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有含有凝胶注射器/步进电机或具有泵的高压室的凝胶递送模块，以通过毛细管快速均匀地输送凝胶。 该系统还包括多波长光束发生器，以产生产生具有宽波长范围的光束的激光束。 离线毛细管修复器彻底清洁毛细管柱，以便同时执行另一个毛细管柱的电泳。 离线毛细管再生器的流线型性质提供了增加系统吞吐量的优点，同时最小化了系统成本。

公开(公告)号：US06352633B1

公开(公告)日：2002-03-05

申请号：US09388125

申请日：1999-08-31

Applicant: Changsheng Liu ,  Thomas E Kane ,  Qingbo Li

Inventor： Changsheng Liu ,  Thomas E Kane ,  Qingbo Li

IPC: G01N2726

CPC classification number: C07K1/26 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782 ,  Y10T436/2575

Abstract: An automated capillary zone electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a container connected to the detection end of the capillaries. The container is provided with valving which facilitate cleaning the capillaries, loading buffer into the capillaries, introducing samples to be electrophoresced into the capillaries, and performing capillary zone electrophoresis on the thus introduced samples.

Abstract translation: 公开了一种自动化毛细管区域电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有连接到毛细管的检测端的容器。 该容器设置有阀，其有助于清洁毛细管，将缓冲液加载到毛细管中，将待电泳的样品引入毛细管中，并在这样引入的样品上进行毛细管区带电泳。

公开(公告)号：US6118127A

公开(公告)日：2000-09-12

申请号：US449418

申请日：1999-11-29

Applicant: Changsheng Liu ,  Qingbo Li

Inventor： Changsheng Liu ,  Qingbo Li

IPC: G01N21/64 ,  G01N27/447

CPC classification number: G01N27/44721

Abstract: A detector for DNA sample identification is provided with a transmission grating beam splitter (TGBS). The TGBS split fluoresced light from a tagged DNA sample into 0th order and a 1st order components, both of which are detected on a two-dimensional detector array of a CCD camera. The 0th and 1st order components are detected along a column of pixels in the detector array, and are spaced apart from one another. The DNA samples are tagged with four fluorescent dyes, one dye specific for each nucleotide, and all four dyes responding in slightly different manner to the same monochromatic excitation signal. The TGBS splits fluoresced incoming light into 0th and 1st order components, which are then spread out among a number of pixels in the detector array. The 1st component of this light is received by pixels whose position relative to the 0th order component depends on the frequency of fluorescence. Thus, the position at which signal energy is detected on the array is indicative of the particular dye, and therefore, the corresponding nucleotide tagged by that dye. Monitoring signal energy at the 0th order pixel and selected 1st order pixels, provides a set of data from which one may then identify the particular nucleotide.

Abstract translation: 用于DNA样品鉴定的检测器具有透射光栅分束器（TGBS）。 TGBS将来自标记的DNA样品的荧光发射到第0阶和第1阶分量，这两个分量在CCD相机的二维检测器阵列上检测。 沿着检测器阵列中的像素列检测第0和第1阶分量，并且彼此间隔开。 DNA样品用四种荧光染料标记，每种核苷酸具有一种染料，所有四种染料以相同的单色激发信号略有不同的方式响应。 TGBS将入射光发射到第0和第1阶分量，然后将其分散在检测器阵列中的多个像素中。 该光的第一分量由相对于第0次分量的位置取决于荧光频率的像素接收。 因此，在阵列上检测到信号能量的位置指示特定染料，因此指示由该染料标记的相应核苷酸。 监测第0级像素和所选第1级像素处的信号能量，提供一组数据，然后可以从中识别特定核苷酸。