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    AGGREGATION AND DISPERSION METHODS OF MAGNETIC PARTICLES, SEPARATION AND DETECTION METHODS USING THE SAME AND DETECTION KIT
    1.
    发明申请
    AGGREGATION AND DISPERSION METHODS OF MAGNETIC PARTICLES, SEPARATION AND DETECTION METHODS USING THE SAME AND DETECTION KIT 有权
    磁性颗粒的聚集和分散方法，使用其的分离和检测方法和检测试剂盒

    公开(公告)号：US20090001025A1

    公开(公告)日：2009-01-01

    申请号：US12147216

    申请日：2008-06-26

    申请人： YUKI TAKAHASHI ,  NORIYUKI OHNISHI ,  XIAOMAO XIE

    发明人： YUKI TAKAHASHI ,  NORIYUKI OHNISHI ,  XIAOMAO XIE

    IPC分类号： B03C1/01

    CPC分类号： G01N33/54333 ,  G01N27/745 ,  G01N2446/84 ,  Y10T428/2998

    摘要： It is intended to provide a method capable of simply aggregating magnetic particles having a surface modified with a thermoresponsive polymer at a given temperature without heating or cooling an aqueous solution containing the magnetic particles, and a separation method and a detection method of a substance to be detected in a sample using the method. The method of separating a substance to be detected from a sample includes the steps of: mixing an adsorbent and the sample in an aqueous solution to adsorb the substance to be detected on the adsorbent, aggregating the adsorbent by changing a salt concentration in the aqueous solution; and collecting the adsorbent from the aqueous solution by a magnetic force, wherein said adsorbent comprises a magnetic particle of an average particle size of 50 to 1000 nm, a surface of which is modified with a thermoresponsive polymer and is immobilized with a substance having an affinity for the substance to be detected.

    摘要翻译： 本发明的目的是提供一种能够在给定温度下简单地聚集具有用热可聚合物改性的表面的磁性颗粒而不加热或冷却含有磁性颗粒的水溶液的方法，以及物质的分离方法和检测方法 使用该方法在样品中检测。 从样品中分离待检测物质的方法包括以下步骤：将吸附剂和样品混合在水溶液中以将待检测物质吸附在吸附剂上，通过改变水溶液中的盐浓度来聚集吸附剂 ; 并通过磁力从水溶液中收集吸附剂，其中所述吸附剂包括平均粒径为50-1000nm的磁性颗粒，其表面用热反应性聚合物改性并用具有亲和力的物质固定 用于检测物质。

    Aggregation and dispersion methods of magnetic particles, separation and detection methods using the same and detection kit
    3.
    发明授权
    Aggregation and dispersion methods of magnetic particles, separation and detection methods using the same and detection kit 有权
    磁性颗粒的聚集和分散方法，使用相同的分离和检测方法和检测试剂盒

    公开(公告)号：US08105493B2

    公开(公告)日：2012-01-31

    申请号：US12147216

    申请日：2008-06-26

    申请人： Yuki Takahashi ,  Noriyuki Ohnishi ,  Xiaomao Xie

    发明人： Yuki Takahashi ,  Noriyuki Ohnishi ,  Xiaomao Xie

    IPC分类号： B03C1/01 ,  H01F1/00

    CPC分类号： G01N33/54333 ,  G01N27/745 ,  G01N2446/84 ,  Y10T428/2998

    摘要： It is intended to provide a method capable of simply aggregating magnetic particles having a surface modified with a thermoresponsive polymer at a given temperature without heating or cooling an aqueous solution containing the magnetic particles, and a separation method and a detection method of a substance to be detected in a sample using the method. The method of separating a substance to be detected from a sample includes the steps of: mixing an adsorbent and the sample in an aqueous solution to adsorb the substance to be detected on the adsorbent, aggregating the adsorbent by changing a salt concentration in the aqueous solution; and collecting the adsorbent from the aqueous solution by a magnetic force, wherein said adsorbent comprises a magnetic particle of an average particle size of 50 to 1000 nm, a surface of which is modified with a thermoresponsive polymer and is immobilized with a substance having an affinity for the substance to be detected.

    摘要翻译： 本发明的目的是提供一种能够在给定温度下简单地聚集具有用热可聚合物改性的表面的磁性颗粒而不加热或冷却含有磁性颗粒的水溶液的方法，以及物质的分离方法和检测方法 使用该方法在样品中检测。 从样品中分离待检测物质的方法包括以下步骤：将吸附剂和样品混合在水溶液中以将待检测物质吸附在吸附剂上，通过改变水溶液中的盐浓度来聚集吸附剂 ; 并通过磁力从水溶液中收集吸附剂，其中所述吸附剂包括平均粒径为50-1000nm的磁性颗粒，其表面用热反应性聚合物改性并用具有亲和力的物质固定 用于检测物质。