公开(公告)号：US20110200986A1

公开(公告)日：2011-08-18

申请号：US13122947

申请日：2009-10-06

申请人： Kun-Lin Yang ,  Changying Xue ,  Saif A. Khan ,  Xinyan Bi

发明人： Kun-Lin Yang ,  Changying Xue ,  Saif A. Khan ,  Xinyan Bi

IPC分类号： G01N33/543 ,  C12Q1/70 ,  G01N33/569 ,  G01N33/573 ,  G01N33/552 ,  G01N33/553 ,  G01N33/544

CPC分类号： G01N21/78 ,  G01N21/05 ,  G01N33/542 ,  G01N2021/0346 ,  G01N2021/7779 ,  G01N2021/8477 ,  Y02A50/53

摘要： There is provided a method, an in vitro method and a detection system for detecting the presence of at least one biological molecule in at least one sample. The method can include, for example, providing a sample and/or a binding agent to a contact portion of a surface by way of at least one microfluidic channel; disposing a liquid crystal at the contact portion; determining whether the orientation of the liquid crystal changes after the sample contacts the binding agent, indicating the presence of the biological molecule; and determining length of bright region of the liquid crystal and/or change in interference color of said liquid crystal, and consequently indicating the quantity of said biological molecule. Also disclosed are methods of detecting biological molecules using at least one 4′-pentyl-biphenyl-4-R, where R may be at least one functional group selected from carboxylic acid, amine, aldehyde, and oligopeptide.

摘要翻译： 提供了一种用于检测至少一种样品中至少一种生物分子的存在的方法，体外方法和检测系统。 该方法可以包括例如通过至少一个微流体通道将样品和/或粘合剂提供给表面的接触部分; 在接触部处设置液晶; 确定样品接触粘合剂后液晶的取向是否发生变化，表明生物分子的存在; 并确定液晶的亮区的长度和/或所述液晶的干涉色的变化，从而表示所述生物分子的数量。 还公开了使用至少一种4'-戊基 - 联苯-4-R来检测生物分子的方法，其中R可以是至少一个选自羧酸，胺，醛和寡肽的官能团。