公开(公告)号：US20090155764A1

公开(公告)日：2009-06-18

申请号：US12337381

申请日：2008-12-17

申请人： Yanmei Qiao ,  Bing Liu ,  Xiangping Meng ,  Wenjuan Xu ,  Mulong Liu

发明人： Yanmei Qiao ,  Bing Liu ,  Xiangping Meng ,  Wenjuan Xu ,  Mulong Liu

IPC分类号： A01N1/02

CPC分类号： G01N33/5094 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25

摘要： A reagent for four-part differentiation of white blood cells is provided. In one embodiment the reagent has an osmolarity below 50 mOsm/kg H2O. A method for differentiating white blood cells using the reagent is also provided. The disclosure provides for a rapid lysis of red blood cells and four-part differentiation of white blood cells. The reagent may be simple in components and a surfactant is not necessary, but optional. A wide range of pH values may be suitable for the reagent.

摘要翻译： 提供了白细胞四部分分化试剂。 在一个实施方案中，试剂的渗透压低于50mOsm / kg H 2 O。 还提供了使用该试剂来分化白细胞的方法。 本公开提供红细胞的快速溶解和白细胞的四部分分化。 试剂可以是简单的组分，并且表面活性剂不是必需的，但是是任选的。 大范围的pH值可能适用于试剂。

公开(公告)号：US20090162830A1

公开(公告)日：2009-06-25

申请号：US12265588

申请日：2008-11-05

申请人： Bing LIU ,  Wenjuan XU ,  Xiangping MENG ,  Yanmei QIAO ,  Mulong LIU

发明人： Bing LIU ,  Wenjuan XU ,  Xiangping MENG ,  Yanmei QIAO ,  Mulong LIU

IPC分类号： A01N1/02

CPC分类号： G01N33/5094 ,  G01N33/52 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125

摘要： A reagent for classification of leukocytes includes (a) at least two cationic surfactants; (b) at least one organic compound bearing a hydrophobic group and an anionic group; (c) a buffer for adjusting pH into a range of approximately 2-8. Also disclosed is a method for classifying leukocytes with the reagent. With the reagent and method, erythrocytes are lysed rapidly and classification of leukocytes into five groups is achieved in the same channel. The reaction may be carried out at approximately between 10-40° C. and scattered light signals may be detected at two angles for measuring the classification of leukocytes into five groups.

摘要翻译： 用于白细胞分类的试剂包括（a）至少两种阳离子表面活性剂; （b）至少一种带有疏水基团和阴离子基团的有机化合物; （c）用于将pH调节到约2-8范围内的缓冲液。 还公开了一种用于将白细胞与该试剂进行分类的方法。 利用试剂和方法，红细胞迅速裂解，白细胞在同一通道中分为5组。 反应可以在大约10-40℃进行，并且可以以两个角度检测散射光信号，以测量白细胞分为五组。

公开(公告)号：US08304243B2

公开(公告)日：2012-11-06

申请号：US13429189

申请日：2012-03-23

申请人： Bing Liu ,  Wenjuan Xu ,  Xiangping Meng ,  Yanmei Qiao ,  Mulong Liu

发明人： Bing Liu ,  Wenjuan Xu ,  Xiangping Meng ,  Yanmei Qiao ,  Mulong Liu

IPC分类号： G01N33/48

CPC分类号： G01N33/5094 ,  G01N33/52 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125

摘要： A reagent for classification of leukocytes includes (a) at least two cationic surfactants; (b) at least one organic compound bearing a hydrophobic group and an anionic group; (c) a buffer for adjusting pH into a range of approximately 2-8. Also disclosed is a method for classifying leukocytes with the reagent. With the reagent and method, erythrocytes are lysed rapidly and classification of leukocytes into five groups is achieved in the same channel. The reaction may be carried out at approximately between 10-40° C. and scattered light signals may be detected at two angles for measuring the classification of leukocytes into five groups.

摘要翻译： 用于白细胞分类的试剂包括（a）至少两种阳离子表面活性剂; （b）至少一种带有疏水基团和阴离子基团的有机化合物; （c）用于将pH调节到约2-8范围内的缓冲液。 还公开了一种用于将白细胞与该试剂进行分类的方法。 利用试剂和方法，红细胞迅速裂解，白细胞在同一通道中分为5组。 反应可以在大约10-40℃进行，并且可以以两个角度检测散射光信号，以测量白细胞分为五组。

公开(公告)号：US20120183992A1

公开(公告)日：2012-07-19

申请号：US13429189

申请日：2012-03-23

申请人： Bing LIU ,  Wenjuan Xu ,  Xiangping Meng ,  Yanmei Qiao ,  Mulong Liu

发明人： Bing LIU ,  Wenjuan Xu ,  Xiangping Meng ,  Yanmei Qiao ,  Mulong Liu

IPC分类号： C12Q1/04

CPC分类号： G01N33/5094 ,  G01N33/52 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125

摘要： A reagent for classification of leukocytes includes (a) at least two cationic surfactants; (b) at least one organic compound bearing a hydrophobic group and an anionic group; (c) a buffer for adjusting pH into a range of approximately 2-8. Also disclosed is a method for classifying leukocytes with the reagent. With the reagent and method, erythrocytes are lysed rapidly and classification of leukocytes into five groups is achieved in the same channel. The reaction may be carried out at approximately between 10-40° C. and scattered light signals may be detected at two angles for measuring the classification of leukocytes into five groups.

摘要翻译： 用于白细胞分类的试剂包括（a）至少两种阳离子表面活性剂; （b）至少一种带有疏水基团和阴离子基团的有机化合物; （c）用于将pH调节到约2-8范围内的缓冲液。 还公开了一种用于将白细胞与该试剂进行分类的方法。 利用试剂和方法，红细胞迅速裂解，白细胞在同一通道中分为5组。 反应可以在大约10-40℃进行，并且可以以两个角度检测散射光信号，以测量白细胞分为五组。

公开(公告)号：US08143064B2

公开(公告)日：2012-03-27

申请号：US12265588

申请日：2008-11-05

申请人： Bing Liu ,  Wenjuan Xu ,  Xiangping Meng ,  Yanmei Qiao ,  Mulong Liu

发明人： Bing Liu ,  Wenjuan Xu ,  Xiangping Meng ,  Yanmei Qiao ,  Mulong Liu

IPC分类号： G01N33/48

CPC分类号： G01N33/5094 ,  G01N33/52 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125

摘要： A reagent for classification of leukocytes includes (a) at least two cationic surfactants; (b) at least one organic compound bearing a hydrophobic group and an anionic group; (c) a buffer for adjusting pH into a range of approximately 2-8. Also disclosed is a method for classifying leukocytes with the reagent. With the reagent and method, erythrocytes are lysed rapidly and classification of leukocytes into five groups is achieved in the same channel. The reaction may be carried out at approximately between 10-40° C. and scattered light signals may be detected at two angles for measuring the classification of leukocytes into five groups.

公开(公告)号：US20110151562A1

公开(公告)日：2011-06-23

申请号：US13016571

申请日：2011-01-28

申请人： Yanmei Qiao ,  Bing Liu ,  Xiangping Meng ,  Wenjuan Xu ,  Mulong Liu

发明人： Yanmei Qiao ,  Bing Liu ,  Xiangping Meng ,  Wenjuan Xu ,  Mulong Liu

IPC分类号： C12N5/078

CPC分类号： G01N33/5094 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25

摘要： A reagent for four-part differentiation of white blood cells is provided. In one embodiment the reagent has an osmolality below 50 mOsm/kg H2O. A method for differentiating white blood cells using the reagent is also provided. The disclosure provides for a rapid lysis of red blood cells and four-part differentiation of white blood cells. The reagent may be simple in components and a surfactant is not necessary, but optional. A wide range of pH values may be suitable for the reagent.

摘要翻译： 提供了白细胞四部分分化试剂。 在一个实施方案中，试剂的重量摩尔渗透压浓度低于50mOsm / kg H 2 O。 还提供了使用该试剂来分化白细胞的方法。 本公开提供红细胞的快速溶解和白细胞的四部分分化。 试剂可以是简单的组分，并且表面活性剂不是必需的，但是是任选的。 大范围的pH值可能适用于试剂。

公开(公告)号：US08163559B2

公开(公告)日：2012-04-24

申请号：US13016571

申请日：2011-01-28

申请人： Yanmei Qiao ,  Bing Liu ,  Xiangping Meng ,  Wenjuan Xu ,  Mulong Liu

发明人： Yanmei Qiao ,  Bing Liu ,  Xiangping Meng ,  Wenjuan Xu ,  Mulong Liu

IPC分类号： G01N33/48 ,  C12N5/078

CPC分类号： G01N33/5094 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25

摘要： A reagent for four-part differentiation of white blood cells is provided. In one embodiment the reagent has an osmolality below 50 mOsm/kg H2O. A method for differentiating white blood cells using the reagent is also provided. The disclosure provides for a rapid lysis of red blood cells and four-part differentiation of white blood cells. The reagent may be simple in components and a surfactant is not necessary, but optional. A wide range of pH values may be suitable for the reagent.

摘要翻译： 提供了白细胞四部分分化试剂。 在一个实施方案中，试剂的重量摩尔渗透压浓度低于50mOsm / kg H 2 O。 还提供了使用该试剂来分化白细胞的方法。 本公开提供红细胞的快速溶解和白细胞的四部分分化。 试剂可以是简单的组分，并且表面活性剂不是必需的，但是是任选的。 大范围的pH值可能适用于试剂。