公开(公告)号：US08163559B2

公开(公告)日：2012-04-24

申请号：US13016571

申请日：2011-01-28

申请人： Yanmei Qiao ,  Bing Liu ,  Xiangping Meng ,  Wenjuan Xu ,  Mulong Liu

发明人： Yanmei Qiao ,  Bing Liu ,  Xiangping Meng ,  Wenjuan Xu ,  Mulong Liu

IPC分类号： G01N33/48 ,  C12N5/078

CPC分类号： G01N33/5094 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25

摘要： A reagent for four-part differentiation of white blood cells is provided. In one embodiment the reagent has an osmolality below 50 mOsm/kg H2O. A method for differentiating white blood cells using the reagent is also provided. The disclosure provides for a rapid lysis of red blood cells and four-part differentiation of white blood cells. The reagent may be simple in components and a surfactant is not necessary, but optional. A wide range of pH values may be suitable for the reagent.

摘要翻译： 提供了白细胞四部分分化试剂。 在一个实施方案中，试剂的重量摩尔渗透压浓度低于50mOsm / kg H 2 O。 还提供了使用该试剂来分化白细胞的方法。 本公开提供红细胞的快速溶解和白细胞的四部分分化。 试剂可以是简单的组分，并且表面活性剂不是必需的，但是是任选的。 大范围的pH值可能适用于试剂。

公开(公告)号：US20090155764A1

公开(公告)日：2009-06-18

申请号：US12337381

申请日：2008-12-17

申请人： Yanmei Qiao ,  Bing Liu ,  Xiangping Meng ,  Wenjuan Xu ,  Mulong Liu

发明人： Yanmei Qiao ,  Bing Liu ,  Xiangping Meng ,  Wenjuan Xu ,  Mulong Liu

IPC分类号： A01N1/02

CPC分类号： G01N33/5094 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25

摘要： A reagent for four-part differentiation of white blood cells is provided. In one embodiment the reagent has an osmolarity below 50 mOsm/kg H2O. A method for differentiating white blood cells using the reagent is also provided. The disclosure provides for a rapid lysis of red blood cells and four-part differentiation of white blood cells. The reagent may be simple in components and a surfactant is not necessary, but optional. A wide range of pH values may be suitable for the reagent.

摘要翻译： 提供了白细胞四部分分化试剂。 在一个实施方案中，试剂的渗透压低于50mOsm / kg H 2 O。 还提供了使用该试剂来分化白细胞的方法。 本公开提供红细胞的快速溶解和白细胞的四部分分化。 试剂可以是简单的组分，并且表面活性剂不是必需的，但是是任选的。 大范围的pH值可能适用于试剂。

公开(公告)号：US08304243B2

公开(公告)日：2012-11-06

申请号：US13429189

申请日：2012-03-23

申请人： Bing Liu ,  Wenjuan Xu ,  Xiangping Meng ,  Yanmei Qiao ,  Mulong Liu

发明人： Bing Liu ,  Wenjuan Xu ,  Xiangping Meng ,  Yanmei Qiao ,  Mulong Liu

IPC分类号： G01N33/48

CPC分类号： G01N33/5094 ,  G01N33/52 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125

摘要： A reagent for classification of leukocytes includes (a) at least two cationic surfactants; (b) at least one organic compound bearing a hydrophobic group and an anionic group; (c) a buffer for adjusting pH into a range of approximately 2-8. Also disclosed is a method for classifying leukocytes with the reagent. With the reagent and method, erythrocytes are lysed rapidly and classification of leukocytes into five groups is achieved in the same channel. The reaction may be carried out at approximately between 10-40° C. and scattered light signals may be detected at two angles for measuring the classification of leukocytes into five groups.

摘要翻译： 用于白细胞分类的试剂包括（a）至少两种阳离子表面活性剂; （b）至少一种带有疏水基团和阴离子基团的有机化合物; （c）用于将pH调节到约2-8范围内的缓冲液。 还公开了一种用于将白细胞与该试剂进行分类的方法。 利用试剂和方法，红细胞迅速裂解，白细胞在同一通道中分为5组。 反应可以在大约10-40℃进行，并且可以以两个角度检测散射光信号，以测量白细胞分为五组。

公开(公告)号：US20120183992A1

公开(公告)日：2012-07-19

申请号：US13429189

申请日：2012-03-23

申请人： Bing LIU ,  Wenjuan Xu ,  Xiangping Meng ,  Yanmei Qiao ,  Mulong Liu

发明人： Bing LIU ,  Wenjuan Xu ,  Xiangping Meng ,  Yanmei Qiao ,  Mulong Liu

IPC分类号： C12Q1/04

CPC分类号： G01N33/5094 ,  G01N33/52 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125

摘要： A reagent for classification of leukocytes includes (a) at least two cationic surfactants; (b) at least one organic compound bearing a hydrophobic group and an anionic group; (c) a buffer for adjusting pH into a range of approximately 2-8. Also disclosed is a method for classifying leukocytes with the reagent. With the reagent and method, erythrocytes are lysed rapidly and classification of leukocytes into five groups is achieved in the same channel. The reaction may be carried out at approximately between 10-40° C. and scattered light signals may be detected at two angles for measuring the classification of leukocytes into five groups.

摘要翻译： 用于白细胞分类的试剂包括（a）至少两种阳离子表面活性剂; （b）至少一种带有疏水基团和阴离子基团的有机化合物; （c）用于将pH调节到约2-8范围内的缓冲液。 还公开了一种用于将白细胞与该试剂进行分类的方法。 利用试剂和方法，红细胞迅速裂解，白细胞在同一通道中分为5组。 反应可以在大约10-40℃进行，并且可以以两个角度检测散射光信号，以测量白细胞分为五组。

公开(公告)号：US08143064B2

公开(公告)日：2012-03-27

申请号：US12265588

申请日：2008-11-05

申请人： Bing Liu ,  Wenjuan Xu ,  Xiangping Meng ,  Yanmei Qiao ,  Mulong Liu

发明人： Bing Liu ,  Wenjuan Xu ,  Xiangping Meng ,  Yanmei Qiao ,  Mulong Liu

IPC分类号： G01N33/48

CPC分类号： G01N33/5094 ,  G01N33/52 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125

摘要： A reagent for classification of leukocytes includes (a) at least two cationic surfactants; (b) at least one organic compound bearing a hydrophobic group and an anionic group; (c) a buffer for adjusting pH into a range of approximately 2-8. Also disclosed is a method for classifying leukocytes with the reagent. With the reagent and method, erythrocytes are lysed rapidly and classification of leukocytes into five groups is achieved in the same channel. The reaction may be carried out at approximately between 10-40° C. and scattered light signals may be detected at two angles for measuring the classification of leukocytes into five groups.

公开(公告)号：US20110151562A1

公开(公告)日：2011-06-23

申请号：US13016571

申请日：2011-01-28

申请人： Yanmei Qiao ,  Bing Liu ,  Xiangping Meng ,  Wenjuan Xu ,  Mulong Liu

发明人： Yanmei Qiao ,  Bing Liu ,  Xiangping Meng ,  Wenjuan Xu ,  Mulong Liu

IPC分类号： C12N5/078

CPC分类号： G01N33/5094 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25

摘要： A reagent for four-part differentiation of white blood cells is provided. In one embodiment the reagent has an osmolality below 50 mOsm/kg H2O. A method for differentiating white blood cells using the reagent is also provided. The disclosure provides for a rapid lysis of red blood cells and four-part differentiation of white blood cells. The reagent may be simple in components and a surfactant is not necessary, but optional. A wide range of pH values may be suitable for the reagent.

摘要翻译： 提供了白细胞四部分分化试剂。 在一个实施方案中，试剂的重量摩尔渗透压浓度低于50mOsm / kg H 2 O。 还提供了使用该试剂来分化白细胞的方法。 本公开提供红细胞的快速溶解和白细胞的四部分分化。 试剂可以是简单的组分，并且表面活性剂不是必需的，但是是任选的。 大范围的pH值可能适用于试剂。

公开(公告)号：US08965747B2

公开(公告)日：2015-02-24

申请号：US13483997

申请日：2012-05-30

申请人： Huaguang Zhang ,  Qiuye Sun ,  Zhenwei Liu ,  Tieyan Zhang ,  Xiangping Meng ,  Qingqi Zhao ,  Jianguo Zhou ,  Jun Yang ,  Dazhong Ma ,  Yan Zhao ,  Xinrui Liu ,  Jing Guo

发明人： Huaguang Zhang ,  Qiuye Sun ,  Zhenwei Liu ,  Tieyan Zhang ,  Xiangping Meng ,  Qingqi Zhao ,  Jianguo Zhou ,  Jun Yang ,  Dazhong Ma ,  Yan Zhao ,  Xinrui Liu ,  Jing Guo

IPC分类号： G06F17/50 ,  H02J3/26 ,  H02J3/14 ,  H02J3/00

CPC分类号： H02J3/26 ,  H02J3/14 ,  H02J2003/007 ,  Y02E40/50 ,  Y02E60/76 ,  Y04S40/22

摘要： An automatic three-phase unbalanced load compensation experimental device includes an automatic compensation device, a load simulation part, and detection, display and control parts. The automatic compensation device includes power capacitors and intelligent grouping compound switches. The detection, display and control part includes a plurality of current transformers, molded case circuit breakers, a three-phase digital display ammeter, a three-phase electric power monitoring instrument, a protocol conversion module, RS-485 buses, Ethernet cables and an upper computer. The load simulation part includes phase A, B and C loads which are in star connection and are same in component and circuit connection, an analog output module and a relay output module. A power monitoring instrument and digital ammeter are connected to the grid by circuit breaker and the plurality of current transformers. The automatic compensation device and load simulation part are connected to the grid in parallel.

摘要翻译： 自动三相不平衡负载补偿实验装置包括自动补偿装置，负载模拟部分，检测，显示和控制部件。 自动补偿装置包括电力电容器和智能分组复合开关。 检测，显示和控制部分包括多个电流互感器，塑壳断路器，三相数字显示电流表，三相电力监控仪器，协议转换模块，RS-485总线，以太网电缆和 上位机 负载模拟部分包括星形连接的相位A，B和C负载，分量和电路连接相同，模拟输出模块和继电器输出模块。 电力监控仪器和数字电流表通过断路器和多个电流互感器连接到电网。 自动补偿装置和负载模拟部分并联连接到电网。

公开(公告)号：US20130054204A1

公开(公告)日：2013-02-28

申请号：US13483997

申请日：2012-05-30

申请人： Huaguang Zhang ,  Qiuye Sun ,  Zhenwei Liu ,  Tieyan Zhang ,  Xiangping Meng ,  Qingqi Zhao ,  Jianguo Zhou ,  Jun Yang ,  Dazhong Ma ,  Yan Zhao ,  Xinrui Liu ,  Jing Guo

发明人： Huaguang Zhang ,  Qiuye Sun ,  Zhenwei Liu ,  Tieyan Zhang ,  Xiangping Meng ,  Qingqi Zhao ,  Jianguo Zhou ,  Jun Yang ,  Dazhong Ma ,  Yan Zhao ,  Xinrui Liu ,  Jing Guo

IPC分类号： G06G7/48 ,  G06F7/60

CPC分类号： H02J3/26 ,  H02J3/14 ,  H02J2003/007 ,  Y02E40/50 ,  Y02E60/76 ,  Y04S40/22

摘要： Disclosed are an automatic three-phase unbalanced load compensation experimental device and its control method. The experimental device comprises an automatic compensation device, a load simulation part, and detection, display and control parts. The automatic compensation device comprises power capacitors and intelligent grouping compound switches; the detection, display and control part comprises current transformers, molded case circuit breakers, a three-phase digital display ammeter, a three-phase electric power monitoring instrument, a protocol conversion module, 485 buses, Ethernet cables and an upper computer; the load simulation part comprises phase A, B and C loads which are in star connection and are same in component and circuit connection, an analog output module and a relay output module. Power monitoring instrument and digital ammeter are connected to the grid by circuit breaker and current transformer. The automatic compensation device and load simulation part are connected to the grid in parallel.

摘要翻译： 公开了一种自动三相不平衡负载补偿实验装置及其控制方法。 实验装置包括自动补偿装置，负载模拟部件，检测，显示和控制部件。 自动补偿装置包括电力电容器和智能分组复合开关; 检测，显示和控制部分包括电流互感器，塑壳断路器，三相数字显示电流表，三相电力监控仪器，协议转换模块，485总线，以太网电缆和上位机; 负载模拟部分包括星形连接中的相A，B和C负载，在组件和电路连接中相同，模拟输出模块和继电器输出模块。 电力监控仪表和数字电流表通过断路器和电流互感器连接到电网。 自动补偿装置和负载模拟部分并联连接到电网。