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    Protein highly producing recombinant animal cell, method for preparing the same, and method for mass-producing protein using the same
    2.
    发明授权
    Protein highly producing recombinant animal cell, method for preparing the same, and method for mass-producing protein using the same 有权
    蛋白质高产重组动物细胞，其制备方法，以及使用该蛋白质大量生产蛋白质的方法

    公开(公告)号：US07829306B2

    公开(公告)日：2010-11-09

    申请号：US10576978

    申请日：2004-10-21

    申请人： Reiko Matsuyama ,  Hiroaki Maeda ,  Hitomi Shirahama ,  Takayuki Imamura ,  Yasuharu Kamachi

    发明人： Reiko Matsuyama ,  Hiroaki Maeda ,  Hitomi Shirahama ,  Takayuki Imamura ,  Yasuharu Kamachi

    IPC分类号： C12P21/06 ,  C12N15/70 ,  C12N15/00

    CPC分类号： C12P21/02 ,  C12N2510/02

    摘要： A gene encoding a production amount-potentiating factor is introduced into an animal cell to transform the cell. Alternatively, a protein production gene and the gene encoding the production amount-potentiating factor are introduced into the animal cell to transform the cell. Herein, as the production amount potentiating factor, there is used a factor having caspase activity inhibiting activity and/or protein biosynthesis activity potentiating action, for example, baculovirus P35. Further, the animal cell is cultured by a culturing method under a condition that apoptosis is not induced, so that a protein is mass-produced.

    摘要翻译： 将编码产生量增加因子的基因导入动物细胞以转化细胞。 或者，将蛋白质生产基因和编码产生量增强因子的基因导入动物细胞以转化细胞。 这里，作为生产量增强因子，使用具有半胱天冬酶活性抑制活性和/或蛋白质生物合成活性增强作用的因子，例如杆状病毒P35。 此外，通过培养方法在不诱导细胞凋亡的条件下培养动物细胞，从而大量生产蛋白质。