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    Protein highly producing recombinant animal cell, method for preparing the same, and method for mass-producing protein using the same
    1.
    发明授权
    Protein highly producing recombinant animal cell, method for preparing the same, and method for mass-producing protein using the same 有权
    蛋白质高产重组动物细胞，其制备方法，以及使用该蛋白质大量生产蛋白质的方法

    公开(公告)号：US07829306B2

    公开(公告)日：2010-11-09

    申请号：US10576978

    申请日：2004-10-21

    申请人： Reiko Matsuyama ,  Hiroaki Maeda ,  Hitomi Shirahama ,  Takayuki Imamura ,  Yasuharu Kamachi

    发明人： Reiko Matsuyama ,  Hiroaki Maeda ,  Hitomi Shirahama ,  Takayuki Imamura ,  Yasuharu Kamachi

    IPC分类号： C12P21/06 ,  C12N15/70 ,  C12N15/00

    CPC分类号： C12P21/02 ,  C12N2510/02

    摘要： A gene encoding a production amount-potentiating factor is introduced into an animal cell to transform the cell. Alternatively, a protein production gene and the gene encoding the production amount-potentiating factor are introduced into the animal cell to transform the cell. Herein, as the production amount potentiating factor, there is used a factor having caspase activity inhibiting activity and/or protein biosynthesis activity potentiating action, for example, baculovirus P35. Further, the animal cell is cultured by a culturing method under a condition that apoptosis is not induced, so that a protein is mass-produced.

    摘要翻译： 将编码产生量增加因子的基因导入动物细胞以转化细胞。 或者，将蛋白质生产基因和编码产生量增强因子的基因导入动物细胞以转化细胞。 这里，作为生产量增强因子，使用具有半胱天冬酶活性抑制活性和/或蛋白质生物合成活性增强作用的因子，例如杆状病毒P35。 此外，通过培养方法在不诱导细胞凋亡的条件下培养动物细胞，从而大量生产蛋白质。

    Process For Producing Recombinant Fibrinogen Highly Producing Cell and Highly Producing Cell
    3.
    发明申请
    Process For Producing Recombinant Fibrinogen Highly Producing Cell and Highly Producing Cell 有权
    生产重组纤维蛋白原高产细胞和高产细胞的方法

    公开(公告)号：US20100151522A1

    公开(公告)日：2010-06-17

    申请号：US10566266

    申请日：2004-07-28

    申请人： Reiko Matsuyama ,  Hiroaki Maeda

    发明人： Reiko Matsuyama ,  Hiroaki Maeda

    IPC分类号： C12P21/04 ,  C12N15/87 ,  C12N5/06 ,  C07K14/00

    CPC分类号： C07K14/75

    摘要： When genes encoding three kinds of proteins constituting fibrinogen, an α chain (or variant of α chain), a β chain and a γ chain (or variant of γ chain) are incorporated into an animal cell, a constitutional ratio of respective genes is such that a γ chain (and/or variant of γ chain) gene is an equal amount to a 1000-fold amount relative to an α chain (and/or variant of α chain) gene and a β chain gene and, further, by using a baculovirus P35 gene, a recombinant fibrinogen highly producing cell is prepared.

    摘要翻译： 当编码构成纤维蛋白原的三种蛋白质的基因，α链（或α链的变体），a＆bgr; 链和γ链（或γ链的变体）并入动物细胞中，各基因的构成比例使得γ链（和/或γ链的变体）基因等于1000倍 相对于α链（和/或α链的变体）基因和bgr的量; 链基因，并且通过使用杆状病毒P35基因，制备重组纤维蛋白原高产细胞。

    Process for producing recombinant fibrinogen highly producing cell and highly producing cell
    4.
    发明授权
    Process for producing recombinant fibrinogen highly producing cell and highly producing cell 有权
    用于生产重组纤维蛋白原高产细胞和高产细胞的方法

    公开(公告)号：US08283137B2

    公开(公告)日：2012-10-09

    申请号：US10566266

    申请日：2004-07-28

    申请人： Reiko Matsuyama ,  Hiroaki Maeda

    发明人： Reiko Matsuyama ,  Hiroaki Maeda

    IPC分类号： C12N1/00 ,  C12N5/10 ,  C12N5/16 ,  C12P21/00

    CPC分类号： C07K14/75

    摘要： When genes encoding three kinds of proteins constituting fibrinogen, an α chain (or variant of α chain), a β chain and a γ chain (or variant of γ chain) are incorporated into an animal cell, a constitutional ratio of respective genes is such that a α chain (and/or variant of γ chain) gene is an equal amount to a 1000-fold amount relative to an α chain (and/or variant of α chain) gene and a β chain gene and, further, by using a baculovirus P35 gene, a recombinant fibrinogen highly producing cell is prepared.

    摘要翻译： 当编码构成纤维蛋白原的三种蛋白质的基因，α链（或α链的变体），a＆bgr; 链和γ链（或γ链的变体）并入动物细胞中，各基因的构成比例使得α链（和/或γ链的变体）基因等于1000倍 相对于α链（和/或α链的变体）基因和bgr的量; 链基因，并且通过使用杆状病毒P35基因，制备重组纤维蛋白原高产细胞。

    Feline cytokine protein
    7.
    发明授权
    Feline cytokine protein 失效
    猫细胞因子蛋白

    公开(公告)号：US06472509B1

    公开(公告)日：2002-10-29

    申请号：US09011143

    申请日：1998-02-04

    申请人： Takayuki Imamura ,  Hiroaki Maeda ,  Takeshi Fujiyasu ,  Yoshitaka Imagawa ,  Sachio Tokiyoshi

    发明人： Takayuki Imamura ,  Hiroaki Maeda ,  Takeshi Fujiyasu ,  Yoshitaka Imagawa ,  Sachio Tokiyoshi

    IPC分类号： C07K1400

    CPC分类号： C07K14/52 ,  A61K38/00

    摘要： A novel feline cytokine protein having the activity to enhance the cytotoxic activity of feline cytotoxic T lymphocytes, a DNA sequence coding for said protein, a recombinant DNA for expressing said protein, an expression vector comprising said recombinant DNA, a transformant which is transformed with said expression vector, a process for preparing said protein by culturing said transformant, and an antibody against said protein are provided. The novel feline cytokine protein of the present invention is a heterologous dimer comprising FLAF p35 and FLAF p40 and can be used for treating feline infectious diseases such as feline herpes virus type 1 (FHV-1) or feline calicivirus (FCV).

    摘要翻译： 一种具有增强猫细胞毒性T淋巴细胞的细胞毒性活性，编码所述蛋白质的DNA序列，表达所述蛋白质的重组DNA，包含所述重组DNA的表达载体的活性的新型猫科动物细胞因子蛋白， 表达载体，通过培养所述转化体制备所述蛋白质的方法和针对所述蛋白质的抗体。 本发明的新型猫科动物细胞因子蛋白是包含FLAF p35和FLAF p40的异源二聚体，可用于治疗猫感染性疾病如1型（FHV-1）或猫杯状病毒（FCV）。

    MEDICAL ADHESIVE
    8.
    发明申请
    MEDICAL ADHESIVE 有权
    医用胶粘剂

    公开(公告)号：US20140018510A1

    公开(公告)日：2014-01-16

    申请号：US14005906

    申请日：2012-03-09

    申请人： Naoaki Kawakami ,  Hiroaki Maeda

    发明人： Naoaki Kawakami ,  Hiroaki Maeda

    IPC分类号： A61L24/04

    CPC分类号： A61L24/046 ,  C08G18/4837 ,  C08G18/4845 ,  C08G18/773 ,  C09J175/04 ,  C09J175/08 ,  C08L75/04

    摘要： The present invention is directed to providing a safe medical adhesive, a cured body of which is hard to be degraded and decomposed and thus stable and the cured body generates less amount of acid, aldehyde, etc. due to degradation/decomposition. The medical adhesive includes a hydrophilic urethane prepolymer (UP) obtained by reacting a polyisocyanate component (A) containing a fluorine-containing non-aromatic polyisocyanate compound (A1) as an essential ingredient and a polyol component (B) containing a hydrophilic polyol (B1) as an essential ingredient, wherein a chlorine content in a chlorine-containing organic compound based on the weight of the hydrophilic urethane prepolymer (UP) is 0.005 wt % or less.

    摘要翻译： 本发明旨在提供一种安全的医用粘合剂，其固化体难以降解和分解，因此稳定，固化体由于降解/分解而产生较少量的酸，醛等。 医用胶粘剂包括通过使含有含氟非芳香族多异氰酸酯化合物（A1）作为必需成分的多异氰酸酯成分（A）与含有亲水性多元醇（B1）的多元醇成分（B）反应而得到的亲水性氨基甲酸酯预聚物（UP） ）作为必要成分，其中，基于亲水性氨基甲酸酯预聚物（UP）的重量，含氯有机化合物中的氯含量为0.005重量％以下。