公开(公告)号：US20060257393A1

公开(公告)日：2006-11-16

申请号：US10539756

申请日：2003-12-19

申请人： Kenji Sasaki ,  Yasuhiko Katsumura

发明人： Kenji Sasaki ,  Yasuhiko Katsumura

IPC分类号： A61K39/395 ,  C07K16/18

CPC分类号： C07K1/066 ,  C07K1/006 ,  C07K1/1077

摘要： A method for protecting a thiol group in a protein having a free cysteine residue, which comprises adding a compound which has a disulfide bond in the molecule and exerts substantially no influence on the activity of the protein.

摘要翻译： 一种用于保护具有游离半胱氨酸残基的蛋白质中的硫醇基团的方法，其包括在分子中加入具有二硫键的化合物，并且对蛋白质的活性基本上没有影响。

公开(公告)号：US20050123532A1

公开(公告)日：2005-06-09

申请号：US10497516

申请日：2002-12-04

申请人： Takaharu Kouno ,  Yasuhiko Katsumura

发明人： Takaharu Kouno ,  Yasuhiko Katsumura

IPC分类号： C12N1/00 ,  C12P21/02 ,  C12P21/08 ,  A61K39/395 ,  C12N5/06 ,  C12P21/04

CPC分类号： C12N1/00 ,  C12P21/02

摘要： A method of producing a protein having free cysteine with the use of a serum-free medium, characterized in that the protein is produced in the activated state; a method of producing a protein by culturing in a serum-free medium in accordance with the above method; a method of activating a protein having free cysteine which has been produced in the inactivated state; and a protein obtained by any of the above methods. This protein shows physicochemical or biological properties comparable to a protein obtained by using a serum medium.

摘要翻译： 使用无血清培养基生产具有游离半胱氨酸的蛋白质的方法，其特征在于，所述蛋白质以活化状态产生; 根据上述方法在无血清培养基中培养产生蛋白质的方法; 激活已经在灭活状态下产生的具有游离半胱氨酸的蛋白质的方法; 和通过任何上述方法获得的蛋白质。 该蛋白质显示与通过使用血清培养基获得的蛋白质相当的物理化学或生物学特性。