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公开(公告)号:US5439650A
公开(公告)日:1995-08-08
申请号:US970941
申请日:1992-11-03
申请人: Akira Tsugita , Masaharu Kamo , Toyoaki Uchida , Ikuo Nanno , Yasuhiro Nomoto , Seitaro Takahashi
发明人: Akira Tsugita , Masaharu Kamo , Toyoaki Uchida , Ikuo Nanno , Yasuhiro Nomoto , Seitaro Takahashi
IPC分类号: G01N33/50 , B01J19/00 , C07D277/20 , C07D277/40 , C40B40/10 , C40B60/14 , G01N1/28 , B01S8/20 , G05F7/00
CPC分类号: B01J19/0053 , B01J19/0046 , G01N1/28 , B01J2219/00286 , B01J2219/0059 , B01J2219/00725 , C40B40/10 , C40B60/14 , Y10S436/807
摘要: An reaction vessel utilizes a sample carrier composed of a magnetic core and a surface coating effective to support sample of protein or peptide. The sample carrier is floated magnetically and positioned within a reaction chamber. Edman reagent is applied to the sample to effect amino acid sequence analysis of protein or peptide from amino-terminal. By such construction, reaction efficiency is increased to produce sequentially thiazolinon amino-acid derivatives to thereby increase number of identified remaining amino acids, thereby enabling microanalysis of sample.
摘要翻译: 反应容器使用由磁芯和表面涂层组成的样品载体,其有效地支持蛋白质或肽的样品。 样品载体磁性浮动并定位在反应室内。 将Edman试剂应用于样品,以从氨基末端进行蛋白质或肽的氨基酸序列分析。 通过这种结构,提高反应效率以依次产生噻唑啉氨基酸衍生物,从而增加鉴定的残留氨基酸的数量,由此可以进行样品的微量分析。