公开(公告)号：US20130288907A1

公开(公告)日：2013-10-31

申请号：US13989031

申请日：2011-09-21

申请人： Ye Du

发明人： Ye Du

IPC分类号： C12Q1/68

CPC分类号： C12Q1/683 ,  C12N15/1093 ,  C12Q1/6869 ,  C12Q1/6883 ,  C12Q2521/313 ,  C12Q2525/191 ,  C40B50/06

摘要： A DNA library, and a preparing method thereof, a method of determining DNA sequence information, an apparatus and a kit for detecting SNPs, and a method for genotyping may be provided. The method for preparing the DNA library may comprise the steps of: digesting a genomic DNA sample using a restriction endonuclease to obtain a digested product, wherein the restriction endonuclease comprises at least one selected from the group consisting of Mbo II and Tsp 45I; separating the digested product to obtain DNA fragments having a length of 100 bp to 1,000 bp; end-repairing the DNA fragments to obtain an end-repaired DNA fragments; adding a base A to the end of the end-repaired DNA fragments to obtain DNA fragments having a terminal base A; and ligating the DNA fragments having the terminal base A with sequencing adaptors to obtain the DNA library.

摘要翻译： 可以提供DNA文库及其制备方法，确定DNA序列信息的方法，用于检测SNP的装置和试剂盒，以及用于基因分型的方法。 制备DNA文库的方法可包括以下步骤：使用限制性内切核酸酶消化基因组DNA样品以获得消化产物，其中所述限制性内切核酸酶包含选自Mbo II和Tsp 45I中的至少一种; 分离消化的产物以获得长度为100bp至1,000bp的DNA片段; 终止修复DNA片段以获得最终修复的DNA片段; 在末端修复的DNA片段的末端添加碱基A以获得具有末端碱基A的DNA片段; 并将具有末端碱基A的DNA片段与测序接头连接以获得DNA文库。

公开(公告)号：US09493821B2

公开(公告)日：2016-11-15

申请号：US13989031

申请日：2011-09-21

申请人： Ye Du ,  Meiru Zhao ,  Ying Chen ,  Jinghua Wu ,  Geng Tian ,  Jun Wang

发明人： Ye Du ,  Meiru Zhao ,  Ying Chen ,  Jinghua Wu ,  Geng Tian ,  Jun Wang

IPC分类号： C12Q1/68 ,  C12N15/10 ,  C40B50/06

CPC分类号： C12Q1/683 ,  C12N15/1093 ,  C12Q1/6869 ,  C12Q1/6883 ,  C12Q2521/313 ,  C12Q2525/191 ,  C40B50/06

摘要： A DNA library, and a preparing method thereof, a method of determining DNA sequence information, an apparatus and a kit for detecting SNPs, and a method for genotyping may be provided. The method for preparing the DNA library may comprise the steps of: digesting a genomic DNA sample using a restriction endonuclease to obtain a digested product, wherein the restriction endonuclease comprises at least one selected from the group consisting of Mbo II and Tsp 45I; separating the digested product to obtain DNA fragments having a length of 100 bp to 1,000 bp; end-repairing the DNA fragments to obtain an end-repaired DNA fragments; adding a base A to the end of the end-repaired DNA fragments to obtain DNA fragments having a terminal base A; and ligating the DNA fragments having the terminal base A with sequencing adaptors to obtain the DNA library.